Evaluation of Antimicrobial and Proliferation of Fibroblast Cells Activities of Citrus Essential Oils

Abstract

BACKGROUND: Citrus species produce essential oils (EOs) containing various chemical components that show many pharmacological activities. AIM: The purpose of this study is to evaluate the chemical content and antimicrobial activity of EO extracted from fruit peels and leaves of Citrus x aurantifolia (“Asam sundai”) and Citrus aurantifolia (lime EOs extracted from fruit peels and leaves). METHODS: The EO was extracted by the hydrodistillation method. The chemical content was determined using gas chromatography in tandem with mass spectroscopy (GC-MS). Antibacterial activity was performed using broth microdilution method, while proliferation of fibroblast cell was carried out using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. RESULTS: The main constituent of the EO of “asam sundai” peel (MAKS) and lime peel (MAKN) was I-limonene, while the EO of “asam sundai” leaves (MADS) was γ-terpinene. MAKN EOs showed stronger antibacterial activity than MAKS and MADS with minimum inhibitory concentration values of 3.12 mg/ml against S. aureus, MRSA, and Pseudomonas aeruginosa and 6.25 mg/ml for Streptococcus mutans and Escherichia coli. Meanwhile, the highest fibroblast cell proliferation activity showed by MAKS EO at concentrations of 10, 1, and 0.1 g/ml with a percentage of the proliferation of more than 100%. CONCLUSION: It can be concluded that the different species of citrus have different chemical compositions and different biological activities.

Analysis of Turmeric (Curcuma Longa Linn) Essential Oil From Different Growing Locations Using Ftir/Gc-MS Spectroscopy Coupled to Chemometrics and Its Wound Healing Activities

“…Fibroblast proliferation in the wound healing process is naturally stimulated by interleukin-Ib (IL-Ib), platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF). In addition, the migration of fibroblasts to the damaged area is stimulated by the transforming growth factor (TGF), which is a growth factor produced by granulation tissue formed during the inflammatory process [4,5].…”