Evaluation of antimicrobial efficacy of 0.5% IKI, 3% NaOCI and 0.2% CHX when used alone and in combination as intracanal irrigants against Enterococcus Faecalis. – An in vitro study

An In Vitro Study: Does Adding Iodine Potassium Iodide and Cetrimide to Calcium Hydroxide Paste Enhance Its Antimicrobial Effect Against Oral Biofilms?

“…IKI, on the other hand, is usually used in a higher concentration of 2% (iodine) in 4% (potassium iodide) in the clinic. The reason for the lower concentration of 0.5% in the present study and previous studies with similar methodology was that higher iodine concentrations mask the fluorescence in CLSM [14][15][16]32]. Therefore, the killing efficacy of IKI in this study may be lower than what can be obtained with a 2/4% solution.…”

“…To the best of our knowledge, this is the first study to use viability staining and CLSM imaging to assess the impact of CH when used alone or in combination with two other antibacterial agents on oral multispecies biofilms at different stages of maturation. The benefit of the model used is that the biofilm can be "cloned" and multiplied from the same source biofilm to produce a virtually endless number of parallel biofilm samples grown under the same conditions [28][29][30][31][32]. This is likely to improve the reliability of the results and comparisons between different medicaments.…”