Evaluation of Antioxidant and Free Radical Scavenging Activities of Oxystelma esculentum in Various In Vitro Models

Abstract

Objective - The present study was aimed to evaluate the antioxidant and free radical scavenging properties of Oxystelma esculentum (MEOE).Methods- The total antioxidant, reducing power, DPPH radical, superoxide anion radical, nitric oxide radical, hydroxyl radical, hydrogen peroxide scavenging and total phenol content in MEOE was carried out.Results - The total antioxidant activity of MEOE increases with increasing concentration. The reducing capability and free radical scavenging activity in DPPH also increased in a dose dependent manner. The MEOE was found to scavenge the superoxide generated by PMS/NADH/NBT system. Moreover, MEOE was found to inhibit the nitric oxide radical generated from sodium nitropruside. MEOE was also found to inhibit the hydroxyl radical generated by Fe3+/ascorbate/EDTA/H2O2 system. The MEOE scavenges the hydrogen peroxide in a dose dependent manner. The amount of total phenolic compounds was also determined.Conclusion - The results suggest that the methanol extract of Oxystelma esculentum proved its strong antioxidant activity and can be used as a natural antioxidant.

Assessment of in Vitro Antioxidant and Free Radical Scavenging Activity of Cajanus cajan

“…Thus, major attention is being given to isolation of natural antioxidants. Presence of natural antioxidants from different kinds of plant materials have been reported (Ashokkumar et al 2008;Owolabi et al 2008) and it has been found that these phytocompounds are of great interest (Bongiorno et al 2008;Naik and Panda 2008). Many indigenous herbal plants have been used as medicines in India (Packer and Ong 1998;Jovanovic and Simic 2000).…”

Qualitative and quantitative characterization of phytoconstituents from stem bark of Stereospermum colais Buch

“…For the qualitative phytochemical analysis of methanol extracts of stem bark, they were prepared using standard procedures, [13] and also elemental analysis of ash of stem bark for the detection of inorganic elements such as magnesium, calcium, potassium, iron, sulfate, phosphate, chloride, carbonate, and nitrate was performed using specific tests. [14] …”

Antioxidant Activity, Total Phenols, Flavonoids and LCMS Profile of Chamaecrista hildebrandtii (Vatke) Lock and Clerodendrum rotundifolium (Oliv.)

“…These free radicals initiate cancer progression through binding via electron pairing with biological macromolecules such as proteins, lipids and DNA in healthy human cells and cause protein and DNA damage, including mutation, coupled with lipid peroxidation [2]. Free radicals and peroxidative damage are implicated in many human and animal pathological disorders including inflammatory ailments and microbial infections [3], neurodegenerative diseases, cancer, cardiovascular disease, hypertension, ischemia/reperfusion injury, atherosclerosis, diabetes mellitus, rheumatoid arthritis, immunosuppression, ageing and hair loss [4,5,6,7,8]. Consequently, there is an urgent need to search for new strategies to fight such health disorders including the use of natural antioxidants available in plants such as vitamins C and E, terpenoids, phenolic acids, tannins, flavonoids, quinones, lignins, stilbenes, coumarins, alkaloids, betalains, selenium, ßcarotene, lycopene, lutein and other carotenoids [9,10,11,12,13].…”