Objective - The present study was aimed to evaluate the antioxidant and free radical scavenging properties of Oxystelma esculentum (MEOE).Methods- The total antioxidant, reducing power, DPPH radical, superoxide anion radical, nitric oxide radical, hydroxyl radical, hydrogen peroxide scavenging and total phenol content in MEOE was carried out.Results - The total antioxidant activity of MEOE increases with increasing concentration. The reducing capability and free radical scavenging activity in DPPH also increased in a dose dependent manner. The MEOE was found to scavenge the superoxide generated by PMS/NADH/NBT system. Moreover, MEOE was found to inhibit the nitric oxide radical generated from sodium nitropruside. MEOE was also found to inhibit the hydroxyl radical generated by Fe3+/ascorbate/EDTA/H2O2 system. The MEOE scavenges the hydrogen peroxide in a dose dependent manner. The amount of total phenolic compounds was also determined.Conclusion - The results suggest that the methanol extract of Oxystelma esculentum proved its strong antioxidant activity and can be used as a natural antioxidant.
This study evaluated the in vitro antioxidant potential of methanol extract of Lippia nodiflora Mich. (Verbenaceae) (MELN). The different antioxidants assays, including total antioxidant activity, reducing power, free radical, superoxide anion radical, hydroxyl radical, hydrogen peroxide, nitric oxide scavenging, and total phenolic content, were studied. MELN exhibited potent total antioxidant activity that increased with increasing amount of extract concentration (50, 100, 200, and 400 µg/mL), which were compared with standard drug α-tocopherol (400 µg/mL). The different concentrations of MELN and α-tocopherol showed inhibition of 49.07%, 58.96%, 63.07%, 68.29%, and 74.59%, respectively, on peroxidation in linoleic acid emulsion. In addition, MELN had effective reducing power, free radical scavenging, superoxide anion radical scavenging, hydroxyl radical scavenging, hydrogen peroxide radical scavenging, and nitric oxide scavenging activity, and total phenolic content depending on concentration. These various antioxidant activities were compared with standard antioxidants such as BHA, BHT, catechin, and α-tocopherol.
Objective:The methanol extract of Lippia nodiflora (MELN) (Verbenaceae) was evaluated for antitumor activity against Ehrlich's ascites carcinoma (EAC) bearing Swiss albino mice.Methods: MELN was administered at 200 and 400mg/kg body weight daily for 9 days after 24 h of tumor inoculation. After 18 h fasting, mice were sacrificed. The present study deals with the effect of MELN on the growth of transplantable murine tumor, life span of EAC bearing hosts. Hematological profiles and biochemical parameters were estimated for hepato-renal function and metabolism. Further, Antioxidant enzyme activities were investigated.Results: MELN showed significant (p < 0.001) decrease in tumor volume, packed cell volume and viable cell count and elevated the life span of EAC tumor bearing mice. Hematological profiles reverted to more/less normal levels in extract treated mice. The extract marginally altered serum enzymes, total proteins and bilirubin compared with that of EAC bearing mice. MELN significantly (p < 0.001) decreased the levels of lipid peroxidation and increased the levels of GSH, CAT and SOD. Conclusion:The results of the present study suggest that MELN exhibited significant antitumor activity may be due to the increase of antioxidant activity in EAC bearing mice.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.