Evaluation of Antioxidant Potential of Stem and Leaf Extracts of Himalayan Tinospora cordifolia Hook.f. &amp; Thomson

Upadhyay, Geetanjali; Tewari, Lalit M.; Tewari, Geeta; Chopra, Neha; Pandey, Naveen; Upadhyay, Santosh Kumar; Gahtori, Rekha

doi:10.2174/1874847302109010002

Cited by 5 publications

(6 citation statements)

References 14 publications

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“…The aqueous stem extract of Tinospora had a phenolic content of 0.425 mg GAE/g of the extract. It was found to be 12.8 mg of GAE/g of dried aqueous extract by Yadav and Aggarwala (18), but in methanolic extract, Upadhayay,G et al (19) reported it to be 17.48mgGAE/g. Its flavonoid content was found to be 0.28 mgof QE/g of the extract.…”

Section: Discussionmentioning

confidence: 97%

Toxicity of Imidacloprid on Peripheral Blood Lymphocytes by MTT Assay and the Ameliorative Effect of Extract of Tinospora cordifolia (Gilloe) Extract

Khanna¹,

Mukherjee²,

Kachi³

et al. 2022

IJAM

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Imidacloprid (IMI) is a widely used insecticide which has a specific affinity for insect neonicotinoid acetylcholine receptors. Like all insecticides which are used in excess it tends to bioaccumulate in the environment. So it was thought worthwhile to study its cytotoxicity to human peripheral blood lymphocytes in concentrations ranging from 1.5mM to 4mM after 2 hours and 18 hours exposure by MTT method. Trypan blue test was also used to determine the percentage of living cells. The ameliorative effect of an extract of the stem in water and ethanolic extract of leaves of Tinospora cordifolia (Thunb.) Miers, was also studied. The viability of the lymphocytes showed a fall with increasing concentrations at an exposure of 2 hours. After 18 hours exposure to the IMI only, the viability showed a significant dose dependent drop. Trypan blue test for viability was also conducted. Addition of Tinospora extract raised the viability significantly at 2 hours of incubation. In fact this increase was greatest at 3.5mM and 4mM concentration of drug. The ameliorative effect was maximum at 2 hours. Addition of Tinospora leaf extract showed a significant increase in cell viability at 18 hours of incubation as compared to values obtained with only the drug. Thus a considerable loss of viability of lymphocytes was seen after exposure to the drug in the selected concentrations but herbal extracts seem to help to make the damage less marked. The cells showed a significant rise in viability when incubated with Tinospora leaf extract only, confirming its supportive action in cell proliferation. However, taking into account the evident fall in cell viability caused by exposure to the considerably dilute concentrations tested, caution is needed to prevent over exposure to the pesticide while spraying.

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Section: Discussionmentioning

confidence: 97%

Toxicity of Imidacloprid on Peripheral Blood Lymphocytes by MTT Assay and the Ameliorative Effect of Extract of Tinospora cordifolia (Gilloe) Extract

Khanna¹,

Mukherjee²,

Kachi³

et al. 2022

IJAM

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“…In their study, the extraction yields were 11 % for water solvent and 3 % for methanol solvent by maceration methods [33]. Meanwhile, the extraction time was chosen 3× 24 h at room temperature in this study, referring to other studies with the same time [32,33]. In addition, the yield extract of Tinospora stem obtained in 7 days is not significantly different from 3 days [35].…”

Section: Description Of Tinospora Cordifoliamentioning

confidence: 90%

“…Evaluation of the antimicrobial activity of the crude extract of Tinospora crispa stem showed different activities. The study showed that the Tinospora crispa stem methanol extract had the activity against some bacteria, and no activity was shown in the 3 fungal species tested [33].…”

Section: Total Phenolic and Flavonoid Contentsmentioning

confidence: 93%

“…For example, the extract of T. crispa has been claimed that the yield percentage of the sonication method has slightly higher than the maceration method [33]. In their study, the extraction yields were 11 % for water solvent and 3 % for methanol solvent by maceration methods [33]. Meanwhile, the extraction time was chosen 3× 24 h at room temperature in this study, referring to other studies with the same time [32,33].…”

Section: Description Of Tinospora Cordifoliamentioning

confidence: 95%

“…The extraction efficiency depends on the extraction time, temperature, extraction method, solvent used, and composition of phytochemicals [29,30]. For example, the extract of T. crispa has been claimed that the yield percentage of the sonication method has slightly higher than the maceration method [33]. In their study, the extraction yields were 11 % for water solvent and 3 % for methanol solvent by maceration methods [33].…”

Section: Description Of Tinospora Cordifoliamentioning

confidence: 97%

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The Potential of Tinospora cordifolia Extracts as Antibacterial Material against Pseudomonas aeruginosa

Royani

Hanafi²,

Julistiono³

et al. 2022

Trends Sci

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Mitigation and control of bacterial corrosion must have been prioritized for failure anticipation by corrosion associated with microorganisms. Using synthetic inhibitor for material protection from corrosion have problems because it is not eco-friendly. This work conducted preliminary studies on T. cordifolia stem extracts at various methanol ratios against P. aeruginosa biofilm as a new eco-friendly inhibitor. The T. cordifolia stem was extracted by the maceration method with a different ratio of methanol solvent (100, 75 and 50 %). The bacterial activity was assessed using the dilution method (MTT assay) to determine the minimum inhibitory concentration (MIC). The total phenolic and flavonoid contents were determined using Follin-Ciocalteu and aluminum chloride (AlCl3) colorimetric, respectively. Meanwhile, the structure of the active compounds in the extract was identified by using the liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The yields of T. cordifolia extracts are 9.45, 7.56 and 8.40 % at 100, 75 and 50 % of methanol ratios, respectively. The total phenolic content (TPC) in crude methanol extract of T. cordifolia is 11.20, 9.46 and 6.56 % for 100, 75 and 50 % of methanol ratios. Meanwhile, the flavonoid content (TFC) was obtained is about 5.25, 0.64 and 0.33 % for 100, 75 and 50 % of methanol concentrations, respectively. The T. cordifolia extract has antibacterial activity against P. aeruginosa in all ratios of methanol concentrations. The minimum inhibitory concentration (MIC) of T. cordifolia methanol extract was found at 4096 µg/mL. Methanol solvent concentration with the most active antibacterial activity of the extract was 50 %, followed by 75 and 100 %. The structures of the active compound in the methanolic extract of T. cordifolia are a phenolic group, and it is in the alkaloid derivatives (Calopiptin, d-Lirioferine (Lirioferine), Moupinamide, Piperanine, and Yuanhunine). HIGHLIGHTS The crude methanol extract of Tinospora cordifolia stem has potential against aeruginosa in the marine environment The minimum inhibitory concentration (MIC) was found at 4096 µg/mL of extract in a ratio of 50:50 (v/v) of methanol to water The compound structures in cordifolia methanol extracts are Calopiptin, d-Lirioferine (Lirioferine), Moupinamide, Piperanine, and Yuanhunine GRAPHICAL ABSTRACT

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Insights into the neuroprotective strategies to alleviate neurodegenerative conditions: Role of Ayurvedic herbs and their bioactives

Malhotra

Sandhir

2023

Ayurvedic Herbal Preparations in Neurological Disorders

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Evaluation of Antioxidant Potential of Stem and Leaf Extracts of Himalayan Tinospora cordifolia Hook.f. & Thomson

Cited by 5 publications

References 14 publications

Toxicity of Imidacloprid on Peripheral Blood Lymphocytes by MTT Assay and the Ameliorative Effect of Extract of Tinospora cordifolia (Gilloe) Extract

Toxicity of Imidacloprid on Peripheral Blood Lymphocytes by MTT Assay and the Ameliorative Effect of Extract of Tinospora cordifolia (Gilloe) Extract

The Potential of Tinospora cordifolia Extracts as Antibacterial Material against Pseudomonas aeruginosa

Insights into the neuroprotective strategies to alleviate neurodegenerative conditions: Role of Ayurvedic herbs and their bioactives

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