Evaluation of Antioxidant, Toxicological and wound healing Properties of Hibiscus rosa-sinensis L. (Malvaceae) ethanolic leaves extract on different Experimental animal models

Abstract

Objective: The present research article was conducted to study the toxicity profile, antioxidant potential and wound healing activity of ethanolic leaves extract of Hibiscus rosa-sinensis L. (ELEHR). Materials and Methods: In-vitro antioxidant properties were assessed using DPPH radical scavenging activity, Nitric oxide scavenging activity, and superoxide radical scavenging activity. Acute toxicity was performed to study the general behavioural pattern of mice and sub-acute toxicity studies were performed to study the toxic effects of the test extract on different biochemical and haematological parameters, body and organ weight and histopathology of liver, pancreas and kidney. Wound healing properties was evaluated using excision, incision, dead space and burn wound model. Results: Strong antioxidant activity was recorded in a concentration dependant manner for ELEHR in all three models. The toxicological evaluation of ELEHR revealed that it has a reasonable safety profile as there was no severe alteration. In the evaluation of wound healing properties, ELEHR treated group showed an enhanced wound contraction rate and epithelisation period in both excision and burn wound models, whereas in incision wound model wound breaking strength was significantly increased in extract treated group compared to control. Histology of the skin of healed excised wound also showed restoration to normal architecture in extract treated group. Wound healing activity was further substantiated by enhanced antioxidants and connective tissue markers which were estimated in dead space wound model. Conclusion: Thus ELEHR proved to be effective in wound healing which may be because of strong antioxidant properties and also possesses reasonable safety profile.

Investigating potential wound healing properties of polysaccharides extracted from Grewia mollis Juss. and Hoheria populnea A. Cunn. (Malvaceae)

“…Bright field images of stained cultures indicated that fibroblasts treated with GG deposited more collagen than control fibroblasts, with dense bundles of collagen apparent at 48 h in the GG-treated cultures (Fig. 3), Similar deposition of collagen was observed in a wound healing study using type-2 diabetic rats treated with a crude methanolic extract, containing polysaccharides, from the Malvaceae plant, Sida cordifolia Linn (Pawar et al, 2016) and is also consistent with in vivo results using Hibiscus rosa-sinensis Linn where extracts have been reported to positively influence mechanisms such as fibroblast migration and collagen synthesis at a wound site (Shivananda Nayak et al, 2007, Bhaskar and Nithya, 2012, Mondal et al, 2016. Conversely, staining of cells treated with GGDS and LB indicated that the levels of collagen deposition were similar to that of the control at both 24 and 48 h post-treatment.…”

Histopathological and Immunohistochemical Study of Antidiabetic Effects of Heracleum persicum Extract İn Experimentally Diabetic Rats

“…21,22 Free radical formation has been reported to be a direct consequence of hyperglycemia. 23 Expression of antioxidant enzymes such as superoxide dismutase, glutathione peroxidase and catalase are known to be in the lowest level in pancreatic islet cells compared to other tissues such as the liver, kidney and adipose tissue.…”

Medicinal plants with wound healing potential

“…Histological observation of excised wound revealed restoration of normal architecture of the skin. In dead space wound, the extract increased superoxide dismutase (SOD) and catalase (CAT) activity, the weight of granulation tissue and hydroxyproline content (Mondal et al, 2016).…”