Evaluation of Antioxidative and Antidiabetic Activity of Bark of Holarrhena Pubescens Wall

Abstract

Objective: The objectives of the study are to screen out various phytochemicals and to evaluate the antioxidant and antidiabetic potential of the stem bark of Holarrhena pubescens Wall (Holarrhena antidysenterica). Materials and Methods:The antioxidant activity was determined by the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity where ascorbic acid was taken as positive control. The antioxidant property was later exploited and the methanolic extract of plant was tested for antihyperglycemic activity in glucose overloaded hyperglycemic mice. The extract was tested for its hypoglycemic activity at two-dose levels, 250 and 500 mg/kg respectively where Glipizide 5 mg/kg was taken as standard reference drug. All results are presented as mean ± SD (Standard Deviation). Significant differences between experimental groups were determined by Student's t-test. Results:The methanolic and water extract showed strong antioxidant activity with inhibition of more than 90% DPPH free radicals at the concentration of 100µg/mL. The hypoglycemic activity of methanolic extract on glucose tolerance test were significant (p <0.05) for the effects of 500 mg/kg after 120 min of treatment and (p <0.01) for 250 mg/kg of extract after half hour of treatment compared to control.

Phytochemical and antioxidant studies of methanol and chloroform extract from leaves of Azadirachta indica A. Juss. in Tropical region of Nepal

“…Methanol extract of A. indica has shown the highest activity followed by its chloroform extract which coincide with the previous study (Sri et al, 2012). The antioxidant activity shown by methanol extract was much higher than that of chloroform extract which may be due to the presence of polar compounds like phenols, flavonoids, etc., that are soluble in methanol (Bhusal et al, 2014).…”

Antibacterial, antifungal, antioxidant, brine shrimp lethality and polyphenolic content of <i>Holarrhena</i> <i>mitis</i> (Vahl) R.Br. ex Roem. & Schult

“…In terms of the chemistry and the biological activity studies in genus Holarrhena, H. antidysenterica has been thoroughly investigated. These studies revealed that its bark, leaves and seeds showed anti-diabetic, antioxidant (Ali et al, 2009;Bhusal et al, 2014;Ganapathy et al, 2011;Hegde and Jaisal, 2014;Korpenwar, 2011;Preethi et al, 2010;Ray, 2014;Sabira et al, 2014;), antibacterial and antifungal activities (Ballal et al, 2001;Preethi et al, 2010;Rath and Padhy, 2014). In addition, seeds have acetylcholinesterase inhibitory activity which is useful for treating neurological disorders (Yang et al, 2012) and has diabetic defense as well as the ability to reduce triglycerides which affect the total cholesterol level (Pathak et al, 2015).…”

In Vitro Investigation and Evaluation of Novel Drug Based on Polyherbal Extract against Type 2 Diabetes

“…Antioxidant Activity. The DPPH radical-scavenging method was used to quantify the antioxidant activity of granules[25]. The freshly prepared 2 ml DPPH methanolic solution (0.1 mM) was incubated with 2 mL of each sample (0.001-1 mg/mL) for 30 min in dark conditions.…”