Evaluation of Antiproliferative Activity, Safety and Biodistribution of Oxaliplatin and 5-Fluorouracil Loaded Lactoferrin Nanoparticles for the Management of Colon Adenocarcinoma: an In Vitro and an In Vivo Study

Ahmed, Farhan; Kumari, Sonali; Kondapi, Anand K.

doi:10.1007/s11095-018-2457-7

Cited by 22 publications

(15 citation statements)

References 51 publications

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“…Different drugs e.g. temozolamide [ 96 ], fluorouracil (5-FU) [ 97 ], Oxaliplatin [ 98 ] and siRNA [ 6 ] have been successfully loaded into Lf by this method ( Table 3 ). The Lf NPs prepared using this method offered several advantages including small particle size 60–90 nm and high drug loading (50% loading).…”

Section: Preparation Techniques Of Lactoferrin Nanocarriersmentioning

confidence: 99%

“…The NPs exhibited enhanced cytotoxicity against COLO-205 cells as shown by lower IC 50 (1.08 and 0.98 μg/ml for Lf-5FU and Lf-oxalo NPs, respectively) compared to free drug (IC 50 of = 2.27 and 2.22 μg/ml for free 5FU and free oxaliplatin, respectively). The nano-formulations showed a 7-fold decrease in AFC (structural deformity which turns into colon adenocarcinoma) compared to a 3-fold decrease by the free drugs in azoxymethane induced AFC in Wistar rats [ 98 ].…”

Section: Pharmaceutical Applicationsmentioning

confidence: 99%

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Lactoferrin, a multi-functional glycoprotein: Active therapeutic, drug nanocarrier & targeting ligand

et al. 2020

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Recent progress in protein-based nanomedicine, inspired by the success of Abraxane® albumin-paclitaxel nanoparticles, have resulted in novel therapeutics used for treatment of challenging diseases like cancer and viral infections. However, absence of specific drug targeting, poor pharmacokinetics, premature drug release, and off-target toxicity are still formidable challenges in the clinic. Therefore, alternative protein-based nanomedicines were developed to overcome those challenges. In this regard, lactoferrin (Lf), a glycoprotein of transferrin family, offers a promising biodegradable well tolerated material that could be exploited both as an active therapeutic and drug nanocarrier. This review highlights the major pharmacological actions of Lf including anti-cancer, antiviral, and immunomodulatory actions. Delivery technologies of Lf to improve its pries and enhance its efficacy were also reviewed. Moreover, different nano-engineering strategies used for fabrication of drug-loaded Lf nanocarriers were discussed. In addition, the use of Lf for functionalization of drug nanocarriers with emphasis on tumor-targeted drug delivery was illustrated. Besides its wide application in oncology nano-therapeutics, we discussed the recent advances of Lf-based nanocarriers as efficient platforms for delivery of anti-parkinsonian, anti-Alzheimer, anti-viral drugs, immunomodulatory and bone engineering applications.

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Section: Preparation Techniques Of Lactoferrin Nanocarriersmentioning

confidence: 99%

Section: Pharmaceutical Applicationsmentioning

confidence: 99%

Lactoferrin, a multi-functional glycoprotein: Active therapeutic, drug nanocarrier & targeting ligand

et al. 2020

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“…Drug-loaded LF nanoparticles were fabricated by water-in-oil method. Particle size of 5-FU-loaded LF nanoparticles and oxaliplatin-loaded LF nanoparticles were 60–90 nm and 65–100 nm, respectively, whereas, they exhibited EE% of about 48.6 and 51.3, respectively [ 67 ]. In vitro studies showed that both 5 FU- and oxaliplatin- loaded LF nanoparticles displayed enhanced antiproliferative activity in human colon adenocarcinoma (COLO-205) cell line in comparison to their counterpart free drugs.…”

Section: Lactoferrin In Nanotechnologymentioning

confidence: 99%

“…Results revealed that nanoformulations demonstrated enhanced anti-tumor effect compared to free drugs associated with lower systemic toxicity. This enhanced effect could be attributed to the improved pharmacokinetic profiles and better tissue biodistribution of drug-loaded LF nanoparticles when compared to free drugs, besides their enhanced cellular uptake due to the active targeting property of LF [ 67 ].…”

Section: Lactoferrin In Nanotechnologymentioning

confidence: 99%

“… [ 66 ] Oxaliplatin or 5-FU Intravenous Enhanced in vivo anti-tumor effect associated with lower toxicity in colon cancer-induced animal model. [ 67 ] Aurora Kinase B (AKB) siRNA Intravenous + free temozolomide In vivo improved anti-tumor and 2.5 fold increase in survival rate in glioblastoma orthotopic mouse model. [ 56 ] Temozolomide Intravenous Better drug pharmacokinetic profile and brain accumulation with a notable decline in tumor volume and enhanced survival in glioma- bearing mice.…”

Section: Lactoferrin In Nanotechnologymentioning

confidence: 99%

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Lactoferrin, a unique molecule with diverse therapeutical and nanotechnological applications

Sabra

Agwa

2020

International Journal of Biological Macromolecules

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Lactoferrin (LF) is a naturally glycoprotein with iron-binding properties and diverse biological applications including; antiviral, anti-inflammatory, antioxidant, anti-cancer and immune stimulating effects. In addition, LF was found to be an ideal nanocarrier for some hydrophobic therapeutics because of its active targeting potential due to overexpression of its receptor on the surface of many cells. Moreover, it was proven to be a good candidate for fabrication of nanocarriers to specifically deliver drugs in case of brain tumors owing to the capability of LF to cross the blood brain barrier (BBB). Consequently, it seems to be a promising molecule with multiple applications in the field of cancer therapy and nanomedicine.

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Naringin attenuates oxaliplatin‐induced nephrotoxicity and hepatotoxicity: A molecular, biochemical, and histopathological approach in a rat model

Ileriturk,

Kandemir

et al. 2023

J Biochem & Molecular Tox

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Oxaliplatin (OXL) is a significant therapy agent for the worldwide increase in cancer cases. Naringin (4′,5,7‐trihydroxy flavonon 7‐rhamnoglucoside, NRG) has a wide range of biological and pharmacological activities, including antioxidant and anti‐inflammatory potentials. This research aimed to investigate NRG activity in OXL‐induced hepatorenal toxicity. Accordingly, OXL (4 mg/kg b.w.) in 5% glucose was injected intraperitoneally on the first, second, fifth, and sixth days, and NRG (50 and 100 mg/kg b.w.) was given orally 30 min before to treatment. Biochemical, genetic, and histological methods were utilized to investigate the function tests, oxidant/antioxidant status, inflammation, apoptosis, and endoplasmic reticulum (ER) stress pathways in kidney and liver tissues. Administration of NRG demonstrated an antioxidant effect by increasing the activities of OXL‐induced reduced antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and decreasing the elevated lipid peroxidation parameter malondialdehyde levels. Nuclear factor‐κB, tumor necrosis factor‐α, interleukin‐1β, and inducible nitric oxide synthase levels increased in OXL administered groups but reduced in NRG‐treated groups. In the OXL‐administered groups, NRG reduced the apoptosis‐inducing factors Caspase‐3 and B‐cell lymphoma 2 (Bcl‐2)‐associated X protein levels, while elevating the antiapoptotic factor Bcl‐2 levels. OXL triggered prolonged ER stress by increasing the levels of ER stress parameters activating transcription factor 6, protein kinase R‐like ER kinase, inositol‐requiring enzyme 1α, and glucose‐regulated protein 78. Therefore, with the NRG administration, this activity was reduced and the ER stress level decreased. Taken together, it was found that OXL induced toxicity by increasing the levels of urea and creatinine, alanine transaminase, aspartate aminotransferase, and alkaline phosphatase activities, inflammation, apoptosis, ER stress, and oxidants in the liver and kidney tissue, and NRG had a protective effect by reversing the deterioration in these pathways.

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Evaluation of Antiproliferative Activity, Safety and Biodistribution of Oxaliplatin and 5-Fluorouracil Loaded Lactoferrin Nanoparticles for the Management of Colon Adenocarcinoma: an In Vitro and an In Vivo Study

Cited by 22 publications

References 51 publications

Lactoferrin, a multi-functional glycoprotein: Active therapeutic, drug nanocarrier & targeting ligand

Lactoferrin, a multi-functional glycoprotein: Active therapeutic, drug nanocarrier & targeting ligand

Lactoferrin, a unique molecule with diverse therapeutical and nanotechnological applications

Naringin attenuates oxaliplatin‐induced nephrotoxicity and hepatotoxicity: A molecular, biochemical, and histopathological approach in a rat model

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