Evaluation of Aptamer Fluorescence Microscopy in the Diagnosis of Pulmonary Tuberculosis

Abstract

We established an aptamer fluorescence microscopy approach for rapid detection of MTB in clinical sputum samples. The use of aptamer probes was proven to significantly increase the sensitivity of sputum smear microscopy.

Radical-Induced Dissociation for Oligonucleotide Sequencing by TiO2/ZnAl-Layered Double Oxide-Assisted Laser Desorption/Ionization Mass Spectrometry

“…Even at a concentration of 1 nmol/μL (five times the concentration used in MALDI-RID analysis), the intensity of the highest signal peak ([M − 5H] 5− ) was only 6 × 10. 5 In addition, the ratio of identified fragment ions with a relative intensity of >10% after CID was <20% (Figure S18b−d) and the sequence coverage was only 85% (Figure S18e and Table S5). By contrast, the ratio and sequence coverage in MALDI-RID were 95.7 and 100%, respectively.…”

“…As shown in Figure S18a, the signal intensity of the precursor ion was quite low due to a large number of multicharge signal peaks upon ESI. Even at a concentration of 1 nmol/μL (five times the concentration used in MALDI-RID analysis), the intensity of the highest signal peak ([M – 5H] 5– ) was only 6 × 10 . In addition, the ratio of identified fragment ions with a relative intensity of >10% after CID was <20% (Figure S18b–d) and the sequence coverage was only 85% (Figure S18e and Table S5).…”

“…These molecules play important roles in storing, processing, and expressing genetic information. Endogenous oligoribonucleotides, such as micro RNA (miRNA) and small interfering RNA (siRNA), are associated with cell apoptosis, proliferation, and differentiation. , Meanwhile, the nucleic acid sequences of exogenous oligoribonucleotides, such as antisense oligonucleotides and aptamers, can be manually designed to enable their specific combination with target molecules, forming antigen–antibody complexes that are widely used in cell imaging, new-drug research and development, and microbial detection . Therefore, the rich sequence information contained within short nucleotide sequences supports many biological functions, although the sequences comprise combinations of only four ribonucleotides or deoxyribonucleotides.…”

“…1,2 Meanwhile, the nucleic acid sequences of exogenous oligoribonucleotides, such as antisense oligonucleotides and aptamers, can be manually designed to enable their specific combination with target molecules, forming antigen−antibody complexes that are widely used in cell imaging, 3 new-drug research and development, 4 and microbial detection. 5 Therefore, the rich sequence information contained within short nucleotide sequences supports many biological functions, although the sequences comprise combinations of only four ribonucleotides or deoxyribonucleotides.…”

Research of childhood tuberculosis in suspected populations by molecular methods: A multicenter study in China

“…For example, smear microscopy suffers from poor sensitivity, and the culture of bacterial colonies on solid media, although potentially with high sensitivity and specificity, is time-consuming (about 6~8 weeks). ( Small and Pai, 2010 ; Zheng et al., 2022 ) These drawbacks often lead to failure or delay in childhood tuberculosis diagnosis. In recent years, molecular diagnostic techniques have become widely used in the diagnosis and epidemiological investigation of infection by bacteria of the mycobacterium tuberculosis complex (MTBC).…”