Evaluation of Aromatic Boronic Acids as Ligands for Measuring Diabetes Markers on Carbon Nanotube Field-Effect Transistors

Stefansson, Steingrimur; Stefansson, Lára A.; Chung, Suk-won; Ko, Kevin; Kwon, Hena H.; Ahn, Saeyoung Nate

doi:10.1155/2012/371487

Cited by 8 publications

(11 citation statements)

References 42 publications

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“…The results shown in Figure 3 demonstrate a bell-shaped response curve of E. coli binding to different concentrations of the antibody, with an optimum antibody coating concentration of around 0.5 nM. Previously, we observed a bell-shaped impedance curve in our study of binding of glycated human serum albumin to hydrophobic boronic acid compounds titrated onto CNT-FET circuits, demonstrating the importance of coating concentrations of the capture moieties for maximal ligand signal [15]. Additionally, a bell-shaped response was also observed titrating pyrene-hydrazide-labeled polyclonal antibodies to human IgG (data not shown).…”

Section: Resultssupporting

confidence: 56%

“…We have used this system to measure serum insulin-like growth factor-1 (IGF-1) in a preclinical mouse model of human Brca1-related breast cancer using immobilized antibodies [14]. Recently, we expanded the repertoire of the CNT-FETs by using small hydrophobic boronic acid compounds to measure glycated human serum albumin, which is a marker for diabetes [15]. One of our goals is to develop this technology to measure multiple biomarkers in real time and to reach these goals we need to adopt and utilize established methodologies, including antibody-based detection.…”

Section: Introductionmentioning

confidence: 99%

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Targeting Antibodies to Carbon Nanotube Field Effect Transistors by Pyrene Hydrazide Modification of Heavy Chain Carbohydrates

Stefansson¹,

Kwon²,

Ahn³

2012

Journal of Nanotechnology

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Many carbon nanotube field-effect transistor (CNT-FET) studies have used immobilized antibodies as the ligand binding moiety. However, antibodies are not optimal for CNT-FET detection due to their large size and charge. Their size can prevent ligands from reaching within the Debye length of the CNTs and a layer of charged antibodies on the circuits can drown out any ligand signal. In an attempt to minimize the antibody footprint on CNT-FETs, we examined whether pyrene hydrazide modification of antibody carbohydrates could reduce the concentration required to functionalize CNT circuits. The carbohydrates are almost exclusively on the antibody Fc region and this site-specific modification could mediate uniform antibody orientation on the CNTs. We compared the hydrazide modification of anti-E. coliO157:H7 polyclonal antibodies to pyrenebutanoic acid succinimidyl ester-coated CNTs and carbodiimide-mediated antibody CNT attachment. Our results show that the pyrene hydrazide modification was superior to those methods with respect to bacteria detection and less than 1 nM labeled antibody was required to functionalize the circuits.

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Section: Resultssupporting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Targeting Antibodies to Carbon Nanotube Field Effect Transistors by Pyrene Hydrazide Modification of Heavy Chain Carbohydrates

Stefansson¹,

Kwon²,

Ahn³

2012

Journal of Nanotechnology

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“…Previously we have shown that SWCNT-FET can be utilized to measure many biological molecules including IGF-1 [43], glycated human serum albumin [44] and hemorrhagic E coli [45]. In the present study we examine the interactions of A40 and Aβ42 peptides with SWCNTs immobilized on silica wafers.…”

Section: Introductionmentioning

confidence: 97%

Specific Binding of Alzheimer's Aβ Peptide Fibrils to Single-Walled Carbon Nanotubes

Stefansson¹,

Knight²,

Ahn³

2012

Nanomaterials and Nanotechnology

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Amyloids constitute a class of protein and protein fragments believed to be involved in the pathologies associated with Alzheimer's, Parkinson's and CreutzfeldtJakob diseases. These proteins can self-assemble into unique fibrillar structures that are resistant to normal protein degradation. Interesting recent developments in the study of amyloid fibrils demonstrate that they bind carbon allotropes. In this study, using single-walled carbon nanotube fieldeffect transistors (SWCNT-FETs), we show that the fibrillar form of Alzheimer's amyloid β (1-40) and (1-42) peptides specifically bind non-functionalized SWCNT in a saturable manner. Both peptides exhibited near identical binding curves with half-maximal binding concentrations of approximately 12 g/ml. Binding of the peptides to SWCNTs was diminished by including dimethyl sulphoxide (DMSO) at concentrations that inhibits fibril formation. Lastly, a monoclonal antibody (BAM-10), which binds to the N-terminal region of Alzheimer's amyloid fibrils, recognizes the amyloid peptides adhering to SWCNTs in the absence of DMSO, but not in the presence of 75% DMSO. Taken together, these results suggest that the fibrillar form of the Alzheimer's amyloid peptides are specifically binding to SWCNTs.

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“…The chromatography samples were applied to the field-effect transistor (FET) circuits according to the procedures of Fuzbien Technology Institute [28][29] to determine semiconductor activity. [30].…”

Section: Introductionmentioning

confidence: 99%

Purification of semiconducting single-walled carbon nanotubes by spiral counter-current chromatography

Knight¹,

Lazo-Portugal²,

Ahn³

et al. 2017

Journal of Chromatography A

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Over the last decade man-made carbon nanostructures have shown great promise in electronic applications, but they are produced as very heterogeneous mixtures with different properties so the achievement of a significant commercial application has been elusive. The dimensions of single-wall carbon nanotubes are generally a nanometer wide, up to hundreds of microns long and the carbon nanotubes have anisotropic structures. They are processed to have shorter lengths but they need to be sorted by diameter and chirality. Thus counter-current chromatography methods developed for large molecules are applied to separate these compounds. A modified mixer-settler spiral CCC rotor made with 3 D printed disks was used with a polyethylene glycol-dextran 2-phase solvent system and a surfactant gradient to purify the major species in a commercial preparation. We isolated the semi-conducting single walled carbon nanotube chiral species identified by UV spectral analysis. The further development of spiral counter-current chromatography instrumentation and methods will enable the scalable purification of carbon nanotubes useful for the next generation electronics.

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Evaluation of Aromatic Boronic Acids as Ligands for Measuring Diabetes Markers on Carbon Nanotube Field-Effect Transistors

Cited by 8 publications

References 42 publications

Targeting Antibodies to Carbon Nanotube Field Effect Transistors by Pyrene Hydrazide Modification of Heavy Chain Carbohydrates

Targeting Antibodies to Carbon Nanotube Field Effect Transistors by Pyrene Hydrazide Modification of Heavy Chain Carbohydrates

Specific Binding of Alzheimer's Aβ Peptide Fibrils to Single-Walled Carbon Nanotubes

Purification of semiconducting single-walled carbon nanotubes by spiral counter-current chromatography

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