Evaluation of Aspergillus niger and Penicillium simplicissimum for their ability to leach Zn–Ni–Cu from waste mobile phone printed circuit boards

Abstract

In this research, Zn, Ni, and Cu recovery from mobile phone printed circuit boards was investigated. The initial pH and pulp density using Aspergillus niger or Penicillium simplicissimum fungi were optimized to improve the recovery of Zn, Ni, and Cu using a central composite design. Fungi were able to recover 97% of Cu. Often for Ni recovery, A. niger was more effective, but in low pulp densities and low pH, P. simplicissimum was preferred. For recovery of Zn, A. niger is more appropriate at pH lower than 6, but P. simplicissimum outperforms at pH higher than 6. Under the optimum conditions (pulp density of 4 gL −1 and initial pH 10), the respective recovery of Cu, Ni, and Zn was determined as 94%, 100%, and 100% using A. niger as well as 100%, 95%, and 87% using P. simplicissimum. At alkaline conditions, oxalic acid, citric acid, and gluconic acid are the main acids produced by A. niger; the main acid produced by P. simplicissimum is oxalic acid. Similarly, FTIR and chemical characteristics of the metabolites (the organic acid produced) were analyzed under optimal conditions using HPLC. A. niger in alkaline and acidic conditions produces more acids which lead to higher recovery.

Biomining of Lunar regolith simulant EAC-1A with the fungus Penicillium simplicissimum

“…To validate P. simplicissimum's capacity to mobilize metals from the regolith to the liquid medium, bioleaching veri cation tests were performed in bioreactor asks by growing fungal cultures in full PDB medium with 60% EAC-1A regolith for 2-weeks (Supplemental Table 2). Additionally, because both low nutrient availability and presence of toxic metals could trigger changes in the fungus's bioleaching potential 17,32 , we investigated the impact of using low nutrient (LN) medium (20% PDB), and a reduced amount of EAC-1A regolith (30%). Culture pro ling parameters were monitored to inform the bioleaching process, these were: medium pH, total iron (Fe 3+ and Fe 2+ ) concentration, and levels of organic acid (citric and oxalic acid) (Fig.…”

“…This happens as lamentous fungi excel at producing and excreting organic acids, performing an organic acid-mediated bioleaching. The fungus Penicillium simplicissimum is currently used to biomine copper and has been reported to effectively mine valuable components from electronic waste 17 . P. simplicissimum bioleaches via the fungus' natural production of organic acids, such as citric or oxalic acid.…”