Evaluation of autoantibody signatures in pituitary adenoma patients using human proteome arrays

Abstract

Purpose To identify the specific diagnostic biomarkers related to pituitary adenomas (PAs), we performed serological antibody profiles for three types of PAs, namely Acromegaly, Cushing's and Nonfunctional Pituitary Adenomas (NFPAs), using the human proteome (HuProt) microarray. This is the first study describing the serum autoantibody profile of PAs. Experimental Design We performed serological autoantibody profiling of four healthy controls, four Acromegaly, three Cushing's and three NFPAs patient samples to obtain their autoantibody profiles, which were used for studying expression, interaction and altered biological pathways. Further, significant autoantibodies of PAs were compared with data available for glioma, meningioma and AAgAtlas for their specificity. Results Autoantibody profile of PAs led to the identification of differentially expressed significant proteins such as AKNAD1 (AT‐Hook Transcription Factor [AKNA] Domain Containing 1), NINJ1 (Nerve injury‐induced protein 1), L3HYPDH (Trans‐3‐hydroxy‐L‐proline dehydratase), RHOG (Rho‐related GTP‐binding protein) and PTP4A1 (Protein Tyrosine Phosphatase Type IVA 1) in Acromegaly. Protein ABR (Active breakpoint cluster region‐related protein), ST6GALNAC6 (ST6 N‐acetylgalactosaminide alpha‐2, 6‐sialyltransferase 6), NOL3 (Nucleolar protein 3), ANXA8 (Annexin A8) and POLR2H (RNA polymerase II, I and III subunit H) showed an antigenic response in Cushing's patient's serum samples. Protein dipeptidyl peptidase 3 (DPP3) and reticulon‐4 (RTN4) exhibited a very high antigenic response in NFPA patients. These proteins hold promise as potential autoantibody biomarkers in PAs.

Bifunctional glycosphingolipid (GSL) probes to investigate GSL-interacting proteins in cell membranes

“… ( 108 , 109 , 110 ) P84095 RHOG A Rho-related GTP-binding protein, which interacts with glycolipid transfer proteins to regulate GSL distribution in different intracellular membranes. ( 111 , 112 ) Q13823 GNL2 A nucleolar GTP-binding protein, which acts as GTPase involved in GSL biosynthesis. ( 113 ) …”

Advances in plasma proteomics: Moving from technology to precision medicine

“…The results identified autoantibodies to five proteins in Acromegaly, five proteins in Cushing's patients, two proteins in NFPA patients. [ 13 ] All these results demonstrate the suitability of protein microarray in discovering circulating antibodies associated with humoral autoimmunity. This could be used to execute systematic studies of human diseases together with genomics, proteomics, and metabolomics.…”

Adaptation of the Porcine Pituitary Transcriptome, Spliceosome and Editome during Early Pregnancy

“…Moreover, we identified the modifications of transcriptomic processes related to IP 3 and DAG metabolism: CDS1 (missense ASE within exon 10 resulting in Thr substitution with Pro), DGKE (downregulated DEG in EP vs. ML comparison), DGKG (upregulated exon 15 skipping), ISYNA1 (downregulated DEG, expression verified with qPCR), MTMR7 (missense ASE within exon 15 resulting in Thr substitution with Lys), PEMT (downregulated exon 3 skipping) and PI4K2B (missense ASE within exon 10 resulting in Val substitution with Met; female corticotroph-specific gene expression [ 56 ]). Furthermore, in the CAMKK1 gene, belonging to a Ca 2+ -triggered signaling cascade and involved in a multitude of cellular processes (proteins confirmed in the human APs [ 85 ]), the occurrence of a missense ASE event within exon 16 was noted, providing a predictable substitution of Lys with Glu. The complexity of the mechanisms involved in IP 3 and DAG production, Ca 2+ release and their signaling pathways, as well as the abundance of mRNA and associated transcriptional modifications during the peri-implantation period, makes it impossible to give a clear indication of the direction of the changes taking place.…”