Evaluation of autoclave procedures for fibre analysis in forage and concentrate feedstuffs

Senger, Clóvis Clenio Diesel; Kozloski, Gilberto Vilmar; Sánchez, Luis Maria Bonnecarrère; Mesquita, F.R.; Alves, Tiago Pansard; Castagnino, D.S.

doi:10.1016/j.anifeedsci.2007.12.008

Cited by 216 publications

(128 citation statements)

References 4 publications

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“…In a preview study it was observed that maximal bacteria colonization of forage particles occurs at this incubation time (KOZLOSKI et al, 2008). A third run was conducted using the same procedures described above, except that after 24 hours of incubation, the bags were treated with neutral detergent solution in autoclave (SENGER et al, 2008), washed in tap water and dried in an air-forced oven at 55 °C.…”

Section: In Vitro Bath Culture For Measuring Forage Degradability Andmentioning

confidence: 99%

“…Total N was assayed by a Kjeldahl method (Method 984.13; ASSOCIATION OF OFFICIAL ANALYTICAL -AOAC, 1997). The neutral detergent fibre (NDF) was analysed according to procedures described by Mertens (2002), and acid detergent fibre (ADF) according to Method 973.18 of AOAC (AOAC, 1997), excepting that the samples were weighed into polyester filter bags (porosity of 25µ) and treated with neutral or acid detergent in an autoclave at 110 o C for 40 min (SENGER et al, 2008 Van Soest et al (1991). Total phenolic compounds were analysed with the FolinCicalteau method described by Makkar (2000).…”

Section: Forage Samples and Chemical Analysismentioning

confidence: 99%

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Relationship between chemical components, bacterial adherence and in vitro fermentation of tropical forage legumes

et al. 2013

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Inclusion of forage legumes in diet may improve tropical ruminant systems productivity and sustainability. However, it is not well stablished which chemical components more impact their nutritional value. The relationship between chemical composition and in vitro fermentation of tropical forage legume was evaluated with the objective of obtaining indicators of their nutritional value. Samples of Crotalaria spectabilis, Cajanus cajan, Macrotyloma axillare, Mucuna aterrina, Stylosantis sp. and Canavalia ensiformis, cut from plants at growth age between 47 to 110 days, were analysed. Total gas production showed negative correlation (P<0.05) with total N (R=-0.51), acid detergent fibre (ADF, R=-0.62) and acid detergent lignin (ADL, R=-0.65), and positive correlation with non-fibre carbohydrates (NFC, R=0.70). Gas production rate was negatively related (P<0.05) to NDF (R=-0.73), ADF (R=-0.62) and ADL (R=-0.74). Ammonia concentration in the incubation medium was positively related (P<0.05) to total (R=0.74) and soluble (R=0.56) N, and negatively related to NFC (R=-0.81). The level of bacterial adhesion on residue of incubation was negatively related (P<0.05) to cell wall components, mainly to ADL (R=-0.57). The inclusion of polyehtylene glycol increased both gas volume and gas production rate whereas decreased ammonia concentration (P<0.05). In conclusion, even at low concentrations tannins impact the in vitro fermentation of tropical legumes. However, among the analyzed chemical components, the ADL was the best indicator of the nutritional value of the tropical forage legumes.Index terms: Gas production, lignin, nutritional value, phenolic compounds, tannins. RESUMOA inclusão de leguminosas na dieta tem o potencial de melhorar a produtividade e sustentabilidade dos sistemas de produção de ruminantes em regiões tropicais. Contudo, não está bem estabelecido quais componentes químicos são os mais determinantes do seu valor nutricional. Este estudo avaliou a relação entre componentes químicos e a digestão in vitro de leguminosas forrageiras tropicais, com o objetivo de obter indicadores de seu potencial nutricional. Foram incluídas amostras de Crotalaria spectabilis, Cajanus cajan, Macrotyloma axillare, Mucuna aterrina, Stylosantis sp. e Canavalia ensiformis cortadas de plantas com idades de crescimento entre 47 a 110 dias. O volume de gases apresentou correlação negativa (P<0,05) com os teores de N (R=-0,51), fibra em detergente ácido (FDA, R=-0.62) e lignina em detergente ácido (LDA, R=-0,65), e positiva com o teor de carboidratos não fibrosos (CNF, R=0,70) das leguminosas. A taxa de produção de gases foi negativamente relacionada (P<0.05) com os teores de FDN (R=-0.73), FDA (R=-0.62) e LDA (R=-0.74). A concentração de amônia no meio foi positivamente correlacionada (P<0.05) com o teor de N total (R=0.74) e N solúvel (R=0.56) e, negativamente, correlacionada com o teor de CNF (R=-0.81). O grau de aderência bacteriana no resíduo de incubação foi negativamente relacionada (P<0.05) com os componentes da parede cel...

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Section: In Vitro Bath Culture For Measuring Forage Degradability Andmentioning

confidence: 99%

Section: Forage Samples and Chemical Analysismentioning

confidence: 99%

Relationship between chemical components, bacterial adherence and in vitro fermentation of tropical forage legumes

et al. 2013

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“…Alternative methods 1 and 2, using small bags for sample conditioning and autoclave as digester, followed the recommendations by Komarek (1993) as to use bags instead of filter crucibles, and by Pell and Schofield (1993), Deschamps (1999), and Senger et al (2008), concerning the use of autoclave. The time and temperature in the autoclave followed the best result proposed by Senger et al (2008).…”

Section: Methodsmentioning

confidence: 99%

“…In each determination, around half gram of sample was weighed, adding 100 mL of detergent solution (acid or neutral) in every digestion step, and lead to boil for one h. The analytical results were obtained considering sample weight -being a gravimetric quantitative determination-through the following formula (1) The three alternative methodologies using autoclave (Pell and Schofield, 1993;Deschamps, 1999;Senger et al, 2008) are simplifications of the original analytical procedures, without altering the principles of the method proposed by Van Soest (1963;1967). The alternative methods differed as to the material used for conditioning the samples during analysis.…”

Section: Methodsmentioning

confidence: 99%

“…In an attempt to optimize this methodology, several alternative methods have been adopted in laboratory routines, but often without ensuring reliability of the results, which requires a systematic evaluation of the analytical procedure to demonstrate its precision and accuracy. The use of autoclaves instead of the conventional digester is an alternative method recommended for NDF and ADF analyses (Pell and Schofield, 1993;Deschamps, 1999;Senger et al, 2008). Through this system, it is possible to analyze the samples collectively, without requiring individual control of the samples.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of laboratory methods to assess fiber contents in feedstuff

Lourenço

Messana

Sader

et al. 2017

Rev Colomb Cienc Pecu

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Physicochemical Properties, Lipid Oxidation, and Fatty Acid Composition of Sausage Prepared with Meat of Young Nellore Bulls Fed a Diet with Lauric Acid

Araújo

Ribeiro

Lima

et al. 2020

Euro J Lipid Sci & Tech

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This study evaluates the quality of sausage obtained from the meat of Nellore cattle fed diets containing different levels (0, 5, 10, and 15 g per kg total DM) of lauric acid (C12:0) from palm kernel cake. A linear reduction (p ≤ 0.05) in lipid oxidation, as reflected by linear decreases in the lightness (L*), yellowness (b*), and saturation (C*) color parameters, is observed on days 7, 14, and 21 of maturation of sausage, and these decreases are accompanied by linear increases in the redness (a*) color and the linoleic (C18:2n–6) and linolenic (C18:3n–3) fatty acid contents. The inclusion of lauric acid in the diet induces linear reductions in the shear force and cooking loss and does not significantly affect various indices, including the centesimal composition, water activity, water holding capacity, composition of most fatty acids (FA), hypocholesterolemic‐to‐hypercholesterolemic FA ratio, atherogenicity, thrombogenicity, and desirable fatty acids. The use of dietary lauric acid from palm kernel cake is recommended at doses up to 15 g per kg because its presence reduces lipid oxidation and improves color parameters, softness, and linoleic and linolenic FA without affecting the FA composition of sausage from Nellore bull's meat.Practical applications: Sausages are products manufactured from chopped or ground meats packaged into animal casings. This product appeared in Brazil through the adaptation of traditional recipes brought by German and Italian immigrant families to weather conditions and the national palate. However, due to its production characteristics, sausage can become a product with lipid characteristics that are undesirable for human consumption. The inclusion of lauric acid (C12:0) in the animal diet might change the biohydrogenation process in the rumen, improve the quality of the deposited FA and reduce lipid oxidation in sausage manufactured from meat. The FA composition and physicochemical properties of sausage predict its acceptance by the consumer market. An optimal advantage would be achieved if these products can be used as not only preservatives but also functional ingredients with antioxidant properties and products for the treatment of metabolic syndrome and cardiovascular diseases through atherogenic action.

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Evaluation of autoclave procedures for fibre analysis in forage and concentrate feedstuffs

Cited by 216 publications

References 4 publications

Relationship between chemical components, bacterial adherence and in vitro fermentation of tropical forage legumes

Relationship between chemical components, bacterial adherence and in vitro fermentation of tropical forage legumes

Comparison of laboratory methods to assess fiber contents in feedstuff

Physicochemical Properties, Lipid Oxidation, and Fatty Acid Composition of Sausage Prepared with Meat of Young Nellore Bulls Fed a Diet with Lauric Acid

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