Evaluation of automated floor cleaning, disinfection, and application methods against Staphylococcus aureus

Tembo, Geraldine M.; Chaggar, Gurpreet K.; Li, Xiaobao; Teska, Peter J.; Oliver, Haley F.

doi:10.1016/j.ajic.2022.07.011

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…For pharmaceutical facilities, and to lesser extent healthcare facilities, the floors are additionally cleansed with disinfectants and/or detergents such as benzalkonium chloride (and other quaternary ammonium compounds), hydrogen peroxide, sodium hypochlorite, and other alternatives (Murtough et al, 2001;Sandle, 2012;Eissa et al, 2014;Han et al, 2021;Tembo et al, 2022). While debated in efficacy (Daschner et al, 1982;Meyer and Cookson, 2010;Suleyman et al, 2018), the surface cleaning agents are often rotated in these facilities to minimize potential microbial resistance and/or selection toward the cleaning conditions (Murtough et al, 2001;Sandle, 2012).…”

Section: Introductionmentioning

confidence: 99%

The Impact of the Space Environment on Microbial Growth and Behavior

2024

Frontiers Research Topics

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Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

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Section: Introductionmentioning

confidence: 99%

The Impact of the Space Environment on Microbial Growth and Behavior

2024

Frontiers Research Topics

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“…For pharmaceutical facilities, and to lesser extent healthcare facilities, the floors are additionally cleansed with disinfectants and/or detergents such as benzalkonium chloride (and other quaternary ammonium compounds), hydrogen peroxide, sodium hypochlorite, and other alternatives ( Murtough et al, 2001 ; Rutala et al, 2008 ; Sandle, 2012 ; Eissa et al, 2014 ; Han et al, 2021 ; Tembo et al, 2022 ). While debated in efficacy ( Daschner et al, 1982 ; Meyer and Cookson, 2010 ; Suleyman et al, 2018 ), the surface cleaning agents are often rotated in these facilities to minimize potential microbial resistance and/or selection toward the cleaning conditions ( Murtough et al, 2001 ; Sandle, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolomic and cultivation insights into the tolerance of the spacecraft-associated Acinetobacter toward Kleenol 30, a cleanroom floor detergent

Mogul

Miller²,

Ramos³

et al. 2023

Front. Microbiol.

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IntroductionStringent cleaning procedures during spacecraft assembly are critical to maintaining the integrity of life-detection missions. To ensure cleanliness, NASA spacecraft are assembled in cleanroom facilities, where floors are routinely cleansed with Kleenol 30 (K30), an alkaline detergent.MethodsThrough metabolomic and cultivation approaches, we show that cultures of spacecraft-associated Acinetobacter tolerate up to 1% v/v K30 and are fully inhibited at ≥2%; in comparison, NASA cleanrooms are cleansed with ~0.8-1.6% K30.ResultsFor A. johnsonii 2P08AA (isolated from a cleanroom floor), cultivations with 0.1% v/v K30 yield (1) no changes in cell density at late-log phase, (2) modest decreases in growth rate (~17%), (3) negligible lag phase times, (4) limited changes in the intracellular metabolome, and (5) increases in extracellular sugar acids, monosaccharides, organic acids, and fatty acids. For A. radioresistens 50v1 (isolated from a spacecraft surface), cultivations yield (1) ~50% survivals, (2) no changes in growth rate, (3) ~70% decreases in the lag phase time, (4) differential changes in intracellular amino acids, compatible solutes, nucleotide-related metabolites, dicarboxylic acids, and saturated fatty acids, and (5) substantial yet differential impacts to extracellular sugar acids, monosaccharides, and organic acids.DiscussionThese combined results suggest that (1) K30 manifests strain-dependent impacts on the intracellular metabolomes, cultivation kinetics, and survivals, (2) K30 influences extracellular trace element acquisition in both strains, and (3) K30 is better tolerated by the floor-associated strain. Hence, this work lends support towards the hypothesis that repeated cleansing during spacecraft assembly serve as selective pressures that promote tolerances towards the cleaning conditions.

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“…Although some may be safe (i.e., Lactobacilli ), others, due to their nature as human pathogens or their number, can be strongly harmful and have to be completely removed [ 40 , 41 , 42 , 43 ]. Surface adhesion or absorption mechanisms by microorganisms can be influenced by several factors, namely temperature, pH, relative humidity of the environment, and surface properties, such as porosity, composition, roughness, and hydrophilicity [ 4 , 44 , 45 ]. Among indoor surfaces, floors are the one of the most susceptible to microbial contamination due to the high probability of exposure to sources of contamination and dirt and to the presence of breakages that can retain moisture and nesting microbial colonies [ 46 ].…”

Section: Introductionmentioning

confidence: 99%

Electrolyzed Hypochlorous Acid (HOCl) Aqueous Solution as Low-Impact and Eco-Friendly Agent for Floor Cleaning and Sanitation

Gessi,

Formaglio,

Semeraro

et al. 2023

IJERPH

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Recently, the use of disinfectants has been becoming a diffused and sometimes indiscriminate practice of paramount importance to limit the spreading of infections. The control of microbial contamination has now been concentrated on the use of traditional agents (i.e., hypochlorite, ozone). However, their prolonged use can cause potential treats, for both human health and environment. Currently, low-impact but effective biocides that are prepared in a way that avoids waste, with a very low toxicity, and safe and easy to handle and store are strongly needed. In this study, produced electrochemically activated hypochlorous (HOCl) acid solutions are investigated and proposed, integrated in a scrubbing machine for floor cleaning treatment. Such an innovative machine has been used for floor cleaning and sanitation in order to evaluate the microbial charge and organic dirt removal capacity of HOCl in comparison with a machine charged with traditional Ecolabel standard detergent. The potential damage on floor materials has also been investigated by means of Scanning Electron Microscope (SEM). A comparative Life Cycle Assessment (LCA) analysis has been carried out for evaluating the sustainability of the use of the HOCl-based and detergent-based machine.

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Evaluation of automated floor cleaning, disinfection, and application methods against Staphylococcus aureus

Cited by 3 publications

References 16 publications

The Impact of the Space Environment on Microbial Growth and Behavior

The Impact of the Space Environment on Microbial Growth and Behavior

Metabolomic and cultivation insights into the tolerance of the spacecraft-associated Acinetobacter toward Kleenol 30, a cleanroom floor detergent

Electrolyzed Hypochlorous Acid (HOCl) Aqueous Solution as Low-Impact and Eco-Friendly Agent for Floor Cleaning and Sanitation

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