Evaluation of bacterial antagonists of Ralstonia solanacearum, causal agent of bacterial wilt of potato

Kheirandish, Zeinab; Harighi, Behrouz

doi:10.1016/j.biocontrol.2015.03.007

Cited by 40 publications

(25 citation statements)

References 23 publications

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“…In addition, the three tested (B. subtilis, B. polymyxa and P. fluorescens) bacteria did not inhibit the growth of R. solanacearum where there is no inhibition zone was recorded when double layer and disk agar method were applied. These results are contrariwise those of Kheirandish and Harighi (2015) who found that different strains of P. fluorescens antagonized the growth of R. solanacerum. Also, these results are on the contrary of those of Kwon and Kim (2014) who demonstrated that B. subtilis suppressed the growth of R. solanacerum.…”

Section: Inhibition Effects and Micssupporting

confidence: 79%

INVESTIGATING THE EFFECT OF SOME ELICITORS ON BROWN ROT DISEASE AND TUBER YIELD OF POTATO (Solanum tuberosum L.)

Ezzat

Moussa

2016

Journal of Productivity and Development

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Brown rot of potato is a worldwide disease that causes huge agricultural and economical losses. Using synthetic chemicals like antimicrobial pesticide may have adverse effects on consumers and on the environment. The recent trend of control plant disease is the application of eco-friendly tools to control disease. In the present investigation, and this study streptomycin, Pleurotus columbinus spent wheat straw (PCSWS), wheat straw (WS), three bacterial species (Bacillus subtillus, B. polymyxa and Pseudomonas fluorescens), plant essential oils of anethole, mustard, caraway and neem were tested to control brown rot disease of potato cv. Spunta in Baramoon Research Station, Dakahlia Governorate, Egypt under field conditions, during the two winter seasons of 2013/14 and 2014/15. The vegetative growth parameters, tuber yield, tuber grading, tuber dry matter, specific gravity and infected tubers were investigated. PCSWS led to significant increase in all studied criteria, compared to check or other treatments. Furthermore, PCSWS or caraway oil gave the best results in reduction of infected tubers in vitro. The direct antibacterial effect of studied treatments against the causal pathogen of brown rot of potato; Ralstonia solanacerum. Only streptomycin, anethole and caraway oil affected the growth of R. solanacearum. Pots experiment was carried out to study the ability of the tested treatments to induce systemic resistance (ISR).Conclusively, Bacillus subtillus, caraway and neem oils significantly increased the production of total phenols, polyphenol oxidase and peroxidase, respectively, which enhanced potato plant health, increased chlorophylls and plant height as well as, decreased disease rating that increased tuber weight after 70 days of planting. Preparation of bacterial pathogen and bacterial bio-agents:Bacterial pathogen (R. solanacearum) and PGPR bio-agents of B. subtilis, B. polymyxa and P. fluorescens were inoculated in nutrient growth Glucose Broth (NGB) and incubated in shaker incubator (28 °C and 120 RPM) for 48 hrs. Bacteria were harvested by centrifugation (10000 rpm/ min for 20 min.), each bacterial species was re-suspend in sterilized tap water and each bacterial suspension was adjusted to 10 8 cfu / ml concentration.R. solanacearum bacterial suspension was used for moist soil in pot experiment before 48 hrs of planting. Bacterial bio-agents' suspensions were used to soaking potato seeds for 30 min. before planting in the field and pot experiments.For preparation the bacterial suspension to in vitro experiments, studied bacteria were seeded on nutrient glucose agar (NGA) slants for 48 hrs, harvested, re-suspended in sterilized tap water and adjusted to 10 8 cfu / ml concentration. Organic wastesPleurotus columbinus spent wheat straw (PCSWS) was provided from Mas Mushroom Co., Talkha, Dakahlia governorate, Egypt. Only, wheat straw (WS) powder was prepard from the same Mushroom Co. that used for P. columbinus cultivation. PCSWS and WS were grinded well to fine powder. PCSWS and WS were used for coating ...

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Section: Inhibition Effects and Micssupporting

confidence: 79%

INVESTIGATING THE EFFECT OF SOME ELICITORS ON BROWN ROT DISEASE AND TUBER YIELD OF POTATO (Solanum tuberosum L.)

Ezzat

Moussa

2016

Journal of Productivity and Development

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“…An alternative approach is to use one of the isolates from this study in addition to other biocontrol agents, such as Bacillus and Streptomyces spp. Several studies have demonstrated that a mixture of bioagents is more effective than a single bioagent for bacterial rot control (Kheirandish & Harighi, ).…”

Section: Discussionmentioning

confidence: 99%

Preliminary report on the study of postharvest fruit rot bacteria and yeasts in Lanzhou Lily (Lilium davidii var. unicolor) in China

Ling

Jiao

et al. 2018

Journal of Phytopathology

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Fruit rot is one of the most important factors affecting the postharvest quality and shelf life of Lanzhou Lily fruits. The aims of this study were to characterize different isolates from Lanzhou Lily using morphological and molecular approaches and to test their pathogenicity in Lanzhou Lily fruit. Different isolates were collected from decayed Lanzhou Lily fruits in Lanzhou in Gansu Province in China during 2016 and 2017. In total, four isolates were obtained and identified based on their DNA sequences of the 16S rDNA and 26S rDNA genes in combination with the morphological characteristics of the cultures and sporulation. Of the four isolates, one was identified as Bacillus safensis, one was Stenotrophomonas maltophilia and the other two isolates were Metschnikowia pulcherrima. The pathogenicity tests showed that all four isolates were pathogenic in Lanzhou Lily fruit. Previously Fusarium tricinctum has been reported to be the primary cause of fruit rot in Lanzhou Lily throughout the world. Our report is the first to show results that indicate that B. safensis, S. maltophilia and M. pulcherrima are pathogenic in Lanzhou Lily in China. This is the first report on the bacteria and yeast that infect Lanzhou Lily fruits.

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“…Subsequently, the vasculature is invaded by the bacterium, resulting in chlorosis, stunting, wilting and ultimately plant death (Tan et al ., ). Several reports have provided insight into how tomato or potato plants are affected by R. solanacearum and rhizosphere microbial communities (Huang et al ., ; Kheirandish & Harighi, ; Kim et al ., ). In contrast, reports on the colonization of R. solanacearum in tobacco roots are rare.…”

Section: Introductionmentioning

confidence: 99%

Factors affecting the virulence of Ralstonia solanacearum and its colonization on tobacco roots

Liu

Cai

et al. 2017

Plant Pathology

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Tobacco bacterial wilt caused by Ralstonia solanacearum is a serious disease affecting tobacco cultivation in southwest China. The response surface methodology was employed to evaluate the optimal conditions of tobacco bacterial wilt, and green fluorescent protein gene (gfp) labelling was applied to monitor the location and survival dynamics of R. solanacearum (Rs::gfp) on tobacco roots and in soil under these optimal conditions. The results showed that the highest wilt incidence was 91.13%, which occurred when the population reached 6.6 × 106 CFU/g soil, the temperature was 30.55 °C, and the humidity was >81.42%. The Rs::gfp densely colonized the root tips and root hairs, and cells of Rs::gfp were observed intermittently in the elongation zone or at the point of the emerging lateral roots. The Rs::gfp number in the rhizosphere soil was 10.75‐, 73.13‐ and 74.86‐times higher than that in the bulk soil at 10, 15 and 20 days after transplantation, respectively. Increased colonization by Rs::gfp was related to the population of the pathogen, the environmental temperature and the humidity in the soil. These three conditions determined whether R. solanacearum would induce tobacco wilt. This is the first study to investigate factors affecting the virulence of a tobacco wilt bacterial pathogen, which is important for conducting field diagnosis and biocontrol of tobacco bacterial wilt.

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Evaluation of bacterial antagonists of Ralstonia solanacearum, causal agent of bacterial wilt of potato

Cited by 40 publications

References 23 publications

INVESTIGATING THE EFFECT OF SOME ELICITORS ON BROWN ROT DISEASE AND TUBER YIELD OF POTATO (Solanum tuberosum L.)

INVESTIGATING THE EFFECT OF SOME ELICITORS ON BROWN ROT DISEASE AND TUBER YIELD OF POTATO (Solanum tuberosum L.)

Preliminary report on the study of postharvest fruit rot bacteria and yeasts in Lanzhou Lily (Lilium davidii var. unicolor) in China

Factors affecting the virulence of Ralstonia solanacearum and its colonization on tobacco roots

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