Evaluation of Bacterial Epiphytes Isolated from Avocado Leaf and Fruit Surfaces for Biocontrol of Avocado Postharvest Diseases

Korsten, Lisé; Jager, Elisabeth S. de; Villiers, E E de; Lourens, A. F.; Kotzé, J. M.; Wehner, F. C.

doi:10.1094/pd-79-1149

Cited by 78 publications

(37 citation statements)

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“…where: PGI -percentage growth inhibition, KR -radial growth of fungal colony in the control (mm), and R1 -the distance of fungal growth from the point of inoculation to the colony margin on the treated dishes in the direction of the antagonist (Korsten et al 1995).…”

Section: Dual Culture Assaymentioning

confidence: 99%

Inhibitory effects of antagonistic bacteria inhabiting the rhizosphere of the sugarbeet plants, on Cercospora beticola Sacc., the causal agent of Cercospora leaf spot disease on sugarbeet

Arzanlou¹,

Mousavi²,

Bakhshi³

et al. 2016

Journal of Plant Protection Research

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Abstract:In the present study, the antagonistic capability of bacterial agents inhabiting the rhizosphere of sugarbeet plants were evaluated against Cercospora beticola Sacc. under laboratory and greenhouse conditions. After preliminary screening using the dual culture method, 14 strains with higher antagonistic capability were selected for further inhibitory assays against C. beticola. Bacterial strains were identified based on the sequence data of the small subunit-rDNA (SSU-rDNA) gene. Based on the SSU sequence data, the identity of bacterial strains were determined as Bacillus (10 strains: RB1, RB2, RB3, RB4, RB5, RB6, RB7, RB8, RB9, RB10), Paenibacillus (two strains: RP1, RP2), Enterobacter (one strain: RE), and Pseudomonas (one strain: RPs). The results obtained in this study showed that in all of the assays (dual culture, volatile and non-volatile metabolites) bacterial antagonists significantly inhibited the growth of C. beticola compared to the control. Bacillus (RB2) showed the highest inhibition rate on C. beticola in all of the assays. Based on the results of the laboratory assays, three bacterial strains RB2 (Bacillus), RPs (Pseudomonas), and RE (Paenibacillus) were selected for greenhouse assays. The experiment was designed based on a completely randomised design (CRD) with the application of antagonists prior to, simultaneously, and after inoculation with C. beticola on sugarbeet leaves. The reduction in disease severity was evaluated seven days after inoculation. The results of greenhouse assays were consistent with the results of laboratory studies. The obtained results showed that bacterial antagonists significantly reduced the disease severity when compared to the control.

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Section: Dual Culture Assaymentioning

confidence: 99%

Inhibitory effects of antagonistic bacteria inhabiting the rhizosphere of the sugarbeet plants, on Cercospora beticola Sacc., the causal agent of Cercospora leaf spot disease on sugarbeet

Arzanlou¹,

Mousavi²,

Bakhshi³

et al. 2016

Journal of Plant Protection Research

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“…[123] It is noteworthy that it is very unlikely that bacteria or fungi and horticultural commodities can ever be kept apart, because some occur epiphytically on plant surfaces in the field, others contaminate commodities by air or in contact with soil in farming, harvest or along the handling process. [9,73] Despite all the improvements in the last decades, priority issues to observe will persist: research on mode of action of the various agents and research on the use of physical, biological or chemical options to prevent or cure postharvest diseases.…”

Section: Rhizopus Stolonifer Erwinia Carotovoramentioning

confidence: 99%

The use of essential oils for postharvest decay control. A review.

Antunes

Cavaco

2010

Flavour & Fragrance J

134

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Food safety is one of the major issues related to fresh fruit and vegetables. The preservation of the 'freshness' quality of these products is relevant due to their economical impact. This chapter is a comprehensive review of the use of essential oils from different sources and their constituents on the control of postharvest decay and overall quality preservation of fresh fruit and vegetables. An overview on the most significant postharvest diseases and their impact, as well as on the mainly used synthetic chemicals and their side-effects has been included. Emphasis has been on the sources of essential oils and their constituents studied up to now, and their effects on controlling postharvest diseases, either in vitro or in vivo, and their effect on overall quality and storage life of fresh commodities. Issues regarding their impact on human health and the environment have also been analysed.

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“…Plates were incubated at 25 °C for one week and the percent growth inhibition (GI) of F. oxysporum calculated (Whipps, 1987): (R1-R2) R1 x 100, where R1 is the farthest radial distance (measured in mm) grown by F. oxysporum, in the direction of the antagonist (the control value), and R2 is the distance of fungal growth from the point of inoculation to the colony margin in the direction of the antagonist. Inhibition of growth was categorized on a 0 to 3 scale: 0 (no inhibition), 1 (1-25%), 2 (26-50%), and 3 (51-75%) (Korsten et al, 1995).…”

Section: Origin Of Microorganismsmentioning

confidence: 99%

Characterization and selection ofBacillus sp. strains, effective biocontrol agents againstFusarium oxysporum f. sp.radicis-lycopersici, the causal agent of Fusarium crown and root rot in tomato

et al. 2009

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-The antagonistic activities of 20 Bacillus isolates were tested with dual culture and greenhouse conditions against Fusarium oxysporum f. sp. radicis-lycopersici (FORL) race 0, the causal agent of Fusarium crown and root rot of tomato. Under dual culture, 10 isolates inhibited mycelial growth > 38% and the most effective inhibited fungal growth > 50%. The 20 Bacillus isolates were tested for production of volatiles, cyanide, antibiotics, and phosphorus solubilisation; 15 isolates produced volatiles that inhibited growth of pathogens, 9 isolates produced cyanide, 10 produced antibiotics, and five solubilised phosphorus. Greenhouse experiments with the same 20 isolates revealed the effectiveness of 12 strains, which increased the percentage of healthy plants in the tested cultivar from 66 to 96%. The best disease control was achieved by isolates B11, B5, B17, and B18. However, B11 and B17 were the only isolates that produced cyanide, antibiotics, solubilised phosphate and showed 44% inhibition of fungal growth. The selected strains could be considered in plant growth promotion and biological disease control.

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Evaluation of Bacterial Epiphytes Isolated from Avocado Leaf and Fruit Surfaces for Biocontrol of Avocado Postharvest Diseases

Cited by 78 publications

References 0 publications

Inhibitory effects of antagonistic bacteria inhabiting the rhizosphere of the sugarbeet plants, on Cercospora beticola Sacc., the causal agent of Cercospora leaf spot disease on sugarbeet

Inhibitory effects of antagonistic bacteria inhabiting the rhizosphere of the sugarbeet plants, on Cercospora beticola Sacc., the causal agent of Cercospora leaf spot disease on sugarbeet

The use of essential oils for postharvest decay control. A review.

Characterization and selection ofBacillus sp. strains, effective biocontrol agents againstFusarium oxysporum f. sp.radicis-lycopersici, the causal agent of Fusarium crown and root rot in tomato

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