Evaluation of BD Vacutainer® Barricor™ blood collection tubes for routine chemistry testing on a Roche Cobas® 8000 Platform

Fournier, Jeffrey E.; Northrup, Victoria; Clark, Claudette; Fraser, Jacqueline; Howlett, Michael; Atkinson, Paul; Shea, Jennifer

doi:10.1016/j.clinbiochem.2018.06.002

Cited by 16 publications

(11 citation statements)

References 18 publications

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“…In contrast, the study by Cadamuro et al (17) when compared to other tubes. The difference in LDH is not attributed to different centrifugation conditions (7,9,17). We excluded all the samples that were haemolysed based on a haemolytic index; therefore, the increase in LDH concentrations in our study was not due to artefactual increase.…”

Section: Discussionmentioning

confidence: 98%

Comparative study of chemical pathology sample collection tubes at the largest hospital in South Africa

Khoza¹,

Ford²,

Buthelezi³

et al. 2021

J Med Biochemistry

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Background: BarricorTM lithium heparin plasma tubes are new blood tubes that have been introduced to overcome the effects of gel in serum separator tubes (SST) and the shortcomings of standard lithium heparin plasma. We aimed to evaluate BarricorTM tubes as an alternative to serum separator tubes and compare the stability between the tubes. Methods: Forty-four paired samples were collected using both BarricorTM and SST. We compared five analytes at baseline (<6h) and after every 24h using the Passing-Bablok and Bland-Altman plots. Aspartate aminotransferase (AST), potassium (K), phosphate (PO4), lactate dehydrogenase (LDH), and creatinine were analysed in both tubes. We calculated the percentage difference for each analyte between the baseline and time intervals to assess analyte stability. The percentage difference was compared to the desirable specification for bias and reference change value (RCV). Results: All analytes were comparable at baseline. Statistical differences (p<0.001) became evident after 24h. PO4, K, and creatinine had a mean difference that exceeded the desirable specification for bias (-9.59%, -9.35%, and -4.59%, respectively). Potassium was stable up to 24h in both tubes. LDH showed better stability in SST (144h vs. 96h). PO4 concentrations were more stable in both tubes with the SST (96h vs. 72h). Creatinine and AST had the longest stability in both tubes compared to other analytes (144h). Conclusions: Data demonstrated variability and similarities in analyte concentrations and stability, respectively in both tubes

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Section: Discussionmentioning

confidence: 98%

Comparative study of chemical pathology sample collection tubes at the largest hospital in South Africa

Khoza¹,

Ford²,

Buthelezi³

et al. 2021

J Med Biochemistry

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“…The Barricor tube has been compared with other tubes [12][13][14][15][16][17][18]. A comparison with PST for 50 analytes in 150 patients revealed that the results for the two tubes were comparable, with increased stability for some analytes in the Barricor tube [12]. In 41 volunteers, the Barricor tube yielded decreased LDH and free hemoglobin levels compared with that in the PST [13].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Barricor Tube in 28 Routine Chemical Tests and Its Impact on Turnaround Time in an Outpatient Clinic

et al. 2021

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“…Mechanical blood separation methods are similarly gaining popularity in clinical chemistry due to their easy applicability compared to the use of a separation gel. The BD Vacutainer® Barricor™ is a mechanically-separated plasma (MSP) tube, and it has shown no obvious bias in steroid hormone measurements versus a gel-based plasma tube in a single study 33 , but this was confined to a select number of five steroids, warranting further investigation.…”

Section: Introductionmentioning

confidence: 99%

Validation of multiplex steroid hormone measurements in prostate cancer using plasma for multimodality biomarker studies

Snaterse

Dessel

Visser

et al. 2020

Preprint

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Background Steroid hormones are essential signaling molecules in prostate cancer (PC). However, many studies focusing on liquid biomarkers fail to take hormonal status of these patients into account. Because steroid measurements are sensitive to bias caused by matrix effects, thus assessing potential matrix effects is an important step in combining ctDNA analysis with hormone status. Methods We investigated the accuracy of multi-steroid hormone profiling in mechanically-separated plasma tubes (MSP) and in CellSave Preservative plasma tubes (CS), that are typically used to obtain ctDNA. We performed multiplex steroid profiling in samples obtained from ten healthy controls and ten castration-resistant prostate cancer patients Results Steroid measurements were comparable between the MSP and serum tubes. A small but consistent decrease of 11 - 18% compared to serum was observed in CS tubes, which was considered to be within the acceptable margin. The minimally residual testosterone levels of castrate patients could be sensitively quantified in both CS and MSP samples. Conclusions We validated the use of MSP and CS tubes for multi-steroid profiling by LC-MS/MS. The optimised use of these samples used in clinical trials will allow us to gain further insight into the steroid metabolism in PC patients.

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Evaluation of BD Vacutainer® Barricor™ blood collection tubes for routine chemistry testing on a Roche Cobas® 8000 Platform

Cited by 16 publications

References 18 publications

Comparative study of chemical pathology sample collection tubes at the largest hospital in South Africa

Comparative study of chemical pathology sample collection tubes at the largest hospital in South Africa

Evaluation of the Barricor Tube in 28 Routine Chemical Tests and Its Impact on Turnaround Time in an Outpatient Clinic

Validation of multiplex steroid hormone measurements in prostate cancer using plasma for multimodality biomarker studies

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