Evaluation of Bioactive Attributes and Emulsification Potential of Exopolysaccharide Produced by a Brown-rot Fungus Fomitopsis meliae AGDP-2

Prajapati, Darshankumar; Bhatt, Ashish; Gupte, Akshaya

doi:10.1007/s12010-022-04257-0

Cited by 4 publications

(4 citation statements)

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“…With regard to oil recovery, food production and storage, agrochemicals, and cosmetics, its commercialization has a broad range of uses as an emulsifier and stabilizer [29]. In the present study, studies on emulsification potential stated that EPSs is a good emulsifier of various vegetable oils and the emulsion formed was relatively stable up to 168 h. Similar results are reported by Prajapati et al [63] who found that the stability of the emulsion was tested for 144 h. It was discovered that EPSs produced from Fomitopsis meliae AGDP-2 was a similarly effective stabilizer to guar gum and xanthan gum, and somewhat more effective than sodium alginate.…”

Section: Discussionsupporting

confidence: 90%

“…The anti-cancer effect of EPSs may be caused by a reduction in DNA synthesis and the ability of cells to proliferate [62]. Microbial EPSs can also exert their anticancer activity through stimulating cell-mediated immune responses, like the antitumor activity of natural killer cells, the proliferation of t-lymphocytes, and the phagocyte capacity of mononuclear cells [63].…”

Section: Discussionmentioning

confidence: 99%

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Optimizations of exopolysaccharide production by Fusarium nygamai strain AJTYC1 and its potential applications as an antioxidant, antimicrobial, anticancer, and emulsifier

El-Mahdy,

Mohamed,

El-Ansary

2023

BMC Microbiol

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Background Exopolysaccharides (EPSs) produced by microbes are recognized as biomacromolecules of great significance. EPSs from fungi are widely used in a variety of biotechnological fields, including medicine, bioremediation, and agriculture. Results In this study, ten fungal isolates were isolated from Kafir El-Dair, Qalubia Governorate, Egypt. Isolate 5 produced more exopolysaccharides than the other examined fungi. According to microscopic morphological traits and genetic confirmation by the 18S rRNA gene, isolate 5 was identified as Fusarium nygamai strain AJTYC1. The present study showed that Czapek’s broth media, which contains 6 g/100 ml of sucrose, 10 g/100 ml of peptone, pH 6, and 1.8 × 105 CFU/ml of inoculum size and is incubated at 30 °C for 9 days, was suitable for the production of EPSs from Fusarium nygamai strain AJTYC1 by using static conditions. Fourier transform infrared (FT-IR) was employed in the characterization of EPSs, which exhibited the presence of carboxyl groups, hydroxyl groups, carbonyl groups, and glycosidic bonds. High-performance liquid chromatography (HPLC) detected that EPSs consist of sucrose and glucose. The scavenging activity indicates that EPSs have good antioxidant activity. The partially purified exopolysaccharides produced from F. nygamai strain AJTYC1 exhibited excellent antioxidant and antimicrobial activity against gram positive, gram negative and fungal strains. The EPSs at a dose of 1000 µg/ml exhibited anticancer activity against colorectal colon cancer (HCT116), breast cancer (MCF7), and hepatocellular cancer cell lines. Moreover, EPSs is an effective emulsifier of a variety of vegetable oils, and the emulsion it produces is generally stable for up to 168 h. Conclusions The production of EPSs from F. nygamai strain AJTYC1 can be used as antioxidants, antimicrobials, anticancer, and emulsifiers.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Optimizations of exopolysaccharide production by Fusarium nygamai strain AJTYC1 and its potential applications as an antioxidant, antimicrobial, anticancer, and emulsifier

El-Mahdy,

Mohamed,

El-Ansary

2023

BMC Microbiol

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“…Based on our findings, there is a regulatory effect of L-EPSs on TOS and TAS levels in PC-12 cells induced by OS with H 2 O 2 . It has been stated in the literature that L-EPS provides balance between TOS and TAS levels, thanks to mechanisms of action such as free radical scavenging [55], metal chelation [56], lipid peroxidation [57], electron or hydrogen donation (to ROS) [58], antioxidant stimulant, and enzyme detoxification activities [59]. This view was supported by our findings in a previous study, which implied that our L-EPSs exhibited antioxidant activities (hydroxyl and superoxide anion scavenging activity, metal ion chelating activity, and lipid peroxidation activity) [35].…”

Section: Discussionmentioning

confidence: 99%

Lactic Acid Bacteria-Derived Exopolysaccharides Mitigate the Oxidative Response via the NRF2-KEAP1 Pathway in PC12 Cells

Şirin

2023

CIMB

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Parabiotics, including L-EPSs, have been administered to patients with neurodegenerative disorders. However, the antioxidant properties of L-EPSs against H2O2-induced oxidative stress in PC12 cells have not been studied. Herein, we aimed to investigate the antioxidant properties of the L-EPSs, their plausible targets, and their mechanism of action. We first determined the amount of L-EPSs in Lactobacillus delbrueckii ssp. bulgaricus B3 and Lactiplantibacillus plantarum GD2 using spectrophotometry. Afterwards, we studied their effects on TDH, TOS/TAS, antioxidant enzyme activities, and intracellular ROS level. Finally, we used qRT-PCR and ELISA to determine the effects of L-EPSs on the NRF2-KEAP1 pathway. According to our results, the L-EPS groups exhibited significantly higher total thiol activity, native thiol activity, disulfide activity, TAS levels, antioxidant enzyme levels, and gene expression levels (GCLC, HO-1, NRF2, and NQO1) than did the H2O2 group. Additionally, the L-EPS groups caused significant reductions in TOS levels and KEAP1 gene expression levels compared with those in the H2O2 group. Our results indicate that H2O2-induced oxidative stress was modified by L-EPSs. Thus, we revealed that L-EPSs, which regulate H2O2-induced oxidative stress, could have applications in the field of neurochemistry.

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“…Oils have great application potential for increasing lipid reservoirs. In general, fatty acids in vegetable oils can be used building blocks for cell membranes (Zang et al 2011 ), but they can also provide energy to organisms through β-oxidation (Brzezinska et al 2022 ), and they can influence fungal metabolism (Prajapati et al 2022 ). Key genes related to fatty acid synthesis and triacylglycerol synthesis overexpressed while also increasing intracellular lipid droplet accumulation, which resulted in a lycopene titer of 70.5 mg per gram of biomass, an increase of 25% over the original strain (Ma et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of fatty acids on intracellular pneumocandin B0 storage in the fermentation of Glarea lozoyensis

Zhang,

Yi,

Zhou

et al. 2023

Bioresour. Bioprocess.

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The natural product pneumocandin B0 is the precursor of the antifungal drug caspofungin. To explore the relationship between pneumocandin B0 and oil. We found that the addition of 1 g/L of oil to the fermentation medium is more conducive to the production of pneumocandin B0. The metabolic reaction mechanism was explored using different fatty acids and the results showed that stearic acid and acetic acid increased the total production of pneumocandin B0 by 22.98% and 9.08%, respectively, as well as increasing the content of intracellular lipid droplets. We also analyzed gene expression and pathway differences between the two different fatty acids using transcriptome analyses. The addition of both acetic acid and stearic acid promoted an active pentose phosphate pathway, providing cells with higher intracellular reducing power. We found that the addition of fatty acids can lead to lipid accumulation, and lipid droplets can sequester lipophilic secondary metabolites such as pneumocandin B0 to reduce cell damage. These results provide novel insights into the relationship between pneumocandin B0 biosynthesis and fatty acids in G. lozoyensis. In addition, this study provides important genetic information for improving the yield of pneumocandin B0 through a strategy of metabolic engineering in the future. Graphical Abstract

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Evaluation of Bioactive Attributes and Emulsification Potential of Exopolysaccharide Produced by a Brown-rot Fungus Fomitopsis meliae AGDP-2

Cited by 4 publications

References 57 publications

Optimizations of exopolysaccharide production by Fusarium nygamai strain AJTYC1 and its potential applications as an antioxidant, antimicrobial, anticancer, and emulsifier

Optimizations of exopolysaccharide production by Fusarium nygamai strain AJTYC1 and its potential applications as an antioxidant, antimicrobial, anticancer, and emulsifier

Lactic Acid Bacteria-Derived Exopolysaccharides Mitigate the Oxidative Response via the NRF2-KEAP1 Pathway in PC12 Cells

Effect of fatty acids on intracellular pneumocandin B0 storage in the fermentation of Glarea lozoyensis

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