Evaluation of bioactivity of Annona muricata, Piper betle and Mentha spicata

Samrot, Antony V.; Sherly, H; Sahiti, K.; Rohan, D . Bennet; Raji, P.

doi:10.24896/eijppr.2016611

Cited by 3 publications

(3 citation statements)

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“…There are several studies to support TLC bioautography analysis for identifying antioxidant molecules. 21,33 Antioxidant activity for the metabolites was checked through TLC bioautography (Fig. -18).…”

Section: Invitro Antioxidant Activity In-vitro Antioxidant Activity By Tlc Bioautographymentioning

confidence: 99%

EXTRACTION, CHARACTERIZATION AND INVITRO BIOACTIVITY EVALUATION OF ALKALOIDS, FLAVONOIDS, SAPONINS AND TANNINS OF Cassia alata, Thespesia populnea, Euphorbia hirta AND Wrightia tinctoria

Raji¹,

Samrot²,

Rohan³

et al. 2019

RJC

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The use of medicinal plants to treat infections as a practice can be witnessed since the prehistoric era. Even as on date three-quarter of the world's population totally depends on plants for their primary health care benefits. In this study, secondary metabolites like alkaloids, flavonoids, tannins and saponins were extracted from four medicinal plants viz. Cassia alata, Thespesia populnea, Euphorbia hirta and Wrightia tinctoria and characterized by UV, TLC, FTIR and GCMS analysis. The metabolites were checked for the antibacterial activity using agar well diffusion method and swarming motility assay against Pseudomonas aeruginosa. The alkaloids and saponins of Cassia alata, Euphorbia hirta, Thespesia populnea and Wrightia tinctorial showed dose-response antibacterial activity. The highest antibacterial activity of 1.9 cm was seen for the tannin of Wrightia tinctoria and saponin of Euphorbia hirtaat 16µg concentration. Flavonoid of Euphorbia hirta, Cassia alata and Wrightia tinctorial showed good percentage inhibition of free radicals in DPPH scavenging assay supportive of the antioxidant property. The highest percentage inhibition of 88.75% was noted for tannin of Euphorbia hirta at 50 µg concentration. Antioxidant activity was also checked qualitatively by TLC bioautography and quantitatively for its reducing power on the free radicals by FRAP and phosphomolybdenum assay. The extracted metabolites especially alkaloids were able to restrict the movement of micro organisms to a confined space which was evident from the swarming test.

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Section: Invitro Antioxidant Activity In-vitro Antioxidant Activity By Tlc Bioautographymentioning

confidence: 99%

EXTRACTION, CHARACTERIZATION AND INVITRO BIOACTIVITY EVALUATION OF ALKALOIDS, FLAVONOIDS, SAPONINS AND TANNINS OF Cassia alata, Thespesia populnea, Euphorbia hirta AND Wrightia tinctoria

Raji¹,

Samrot²,

Rohan³

et al. 2019

RJC

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“…Similarly, the low correlation value observed between the total phenolics and metal chelating ability might be explained by the action of the non-phenolic chelators in the tested extracts. Despite there being several studies about the antioxidant properties of M. spicata in the literature, little is known about the influence of the extraction method and the solvent (especially acetone/water) on the extract composition [58][59][60].…”

Section: Antioxidant Propertiesmentioning

confidence: 99%

Novel Perceptions on Chemical Profile and Biopharmaceutical Properties of Mentha spicata Extracts: Adding Missing Pieces to the Scientific Puzzle

Zengin

Ceylan

et al. 2022

Plants

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Mentha spicata is one of the most popular species in the genus, and it is of great interest as a gastrointestinal and sedative agent in the folk medicine system. In this study, different M. spicata extracts, obtained by the use of four solvents (hexane, chloroform, acetone and acetone/water) were chemically characterized using HPLC-ESI-MS n, which allowed for identification of 27 phenolic compounds. The extracts’ antioxidant and enzyme inhibitory properties were investigated. In addition, neuroprotective effects were evaluated in hypothalamic HypoE22 cells, and the ability of the extracts to prevent the hydrogen peroxide-induced degradation of dopamine and serotonin was observed. The best antioxidant effect was achieved for all the extraction methods using acetone/water as a solvent. These extracts were the richest in acacetin, eriodictyol, hesperidin, sagerinic acid, naringenin, luteolin, chlorogenic acid, chrysoeriol and apigenin. The intrinsic antioxidant and enzyme inhibition properties of the acetone/water extract could also explain, albeit partially, its efficacy in preventing prostaglandin E2 overproduction and dopamine depletion (82.9% turnover reduction) in HypoE22 cells exposed to hydrogen peroxide. Thus, our observations can provide a scientific confirmation of the neuromodulatory and neuroprotective effects of M. spicata.

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“… 19 Among many enzymes, α-amylase is a crucial carbohydrate digesting enzyme that is also involved in the hydrolysis of α-(1,4)-glycosidic bonds in starch. 20 As a result, α-amylase is regarded as one of the most promising biological targets for the development of type II diabetes treatment drugs. 21 Urease (urea amidohydrolase, EC 3.5.1.5) is found across numerous plants, animals, fungi and bacteria.…”

Section: Introductionmentioning

confidence: 99%

Novel pyrazoline linked acyl thiourea pharmacophores as antimicrobial, urease, amylase and α-glucosidase inhibitors: design, synthesis, SAR and molecular docking studies

Saeed,

Ahmed,

Haider

et al. 2024

RSC Adv.

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Evaluation of bioactivity of Annona muricata, Piper betle and Mentha spicata

Cited by 3 publications

References 0 publications

EXTRACTION, CHARACTERIZATION AND INVITRO BIOACTIVITY EVALUATION OF ALKALOIDS, FLAVONOIDS, SAPONINS AND TANNINS OF Cassia alata, Thespesia populnea, Euphorbia hirta AND Wrightia tinctoria

EXTRACTION, CHARACTERIZATION AND INVITRO BIOACTIVITY EVALUATION OF ALKALOIDS, FLAVONOIDS, SAPONINS AND TANNINS OF Cassia alata, Thespesia populnea, Euphorbia hirta AND Wrightia tinctoria

Novel Perceptions on Chemical Profile and Biopharmaceutical Properties of Mentha spicata Extracts: Adding Missing Pieces to the Scientific Puzzle

Novel pyrazoline linked acyl thiourea pharmacophores as antimicrobial, urease, amylase and α-glucosidase inhibitors: design, synthesis, SAR and molecular docking studies

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