EXTRACTION, CHARACTERIZATION AND INVITRO BIOACTIVITY EVALUATION OF ALKALOIDS, FLAVONOIDS, SAPONINS AND TANNINS OF Cassia alata, Thespesia populnea, Euphorbia hirta AND Wrightia tinctoria

Abstract

The use of medicinal plants to treat infections as a practice can be witnessed since the prehistoric era. Even as on date three-quarter of the world's population totally depends on plants for their primary health care benefits. In this study, secondary metabolites like alkaloids, flavonoids, tannins and saponins were extracted from four medicinal plants viz. Cassia alata, Thespesia populnea, Euphorbia hirta and Wrightia tinctoria and characterized by UV, TLC, FTIR and GCMS analysis. The metabolites were checked for the antibacterial activity using agar well diffusion method and swarming motility assay against Pseudomonas aeruginosa. The alkaloids and saponins of Cassia alata, Euphorbia hirta, Thespesia populnea and Wrightia tinctorial showed dose-response antibacterial activity. The highest antibacterial activity of 1.9 cm was seen for the tannin of Wrightia tinctoria and saponin of Euphorbia hirtaat 16µg concentration. Flavonoid of Euphorbia hirta, Cassia alata and Wrightia tinctorial showed good percentage inhibition of free radicals in DPPH scavenging assay supportive of the antioxidant property. The highest percentage inhibition of 88.75% was noted for tannin of Euphorbia hirta at 50 µg concentration. Antioxidant activity was also checked qualitatively by TLC bioautography and quantitatively for its reducing power on the free radicals by FRAP and phosphomolybdenum assay. The extracted metabolites especially alkaloids were able to restrict the movement of micro organisms to a confined space which was evident from the swarming test.

Green Synthesis and Antibacterial Activity Studies of Silver Nanoparticles from the Aqueous Extracts of Euphorbia hirta

“…The microorganisms Pseudomonas aeruginosa and Bacillus subtilis were co-cultured in a medium supplemented with silver nanoparticles (0.5µg/ mL) to assess the effect of motility in microbes 17 .…”

ISOLATION AND DETERMINATION OF CHEMICAL COMPOUNDS FROM N-HEXANE FRACTION OF THE SINTOK STEM BARK (Cinnamomum sintoc Bl.)

“…Therefore, to ensure the identity of the isolates it was necessary to compare the isolates of FH3-3 with juniper camphor based on the literature. Araliaceae [34][35][36][37][38] . The alleged identity of isolates was reinforced by the similarity of the fragmentation pattern of FH3-3 isolate mass with juniper camphor.…”

BIOSYNTHESIS OF FUNCTIONALIZED GOLD NANOPARTICLES BY USING METHYL COMMATE C IN Scoparia dulcis LEAF EXTRACT AS REDUCING AGENT

“…8,10 It is well established that plants and its phytochemicals are used in various biomedical applications. 11,12 Plants materials having property to cure various disorders in human beings or in animals with lower or no adverse effects can be termed as medicinal plants. S. dulcis plants have been traditionally used as remedies for diabetes mellitus in India both as fresh and dried form.…”