The aqueous extracts of Euphorbia hirta was used to synthesise silver nanoparticles using bioreduction method. The nanoparticles were characterised by UV Vis spectroscopic analysis, SEM, EDX, AFM, XRD analysis. The silver nanoparticles were also tested for antibacterial activity in Pseudomonas aeruginosa and Bacillus subtilis. The minimum inhibitory concentration for the synthesised nano particles were also tested against the two bacterial species for the least concentration of 0.5µg/mL. The swarming motility assay and protein leakage assay was also tested for the nanoparticle. The silver nanoparticles were found to be much effective.
Silver nano particles synthesised from the leaves of Wrightia tinctoria were characterised by following instrumental analysis -UV Vis spectroscopy, Scanning Electron Microscopy, Energy dispersive X-ray spectroscopy, Atomic force microscopy and X-ray diffraction. The interaction of the silver nanoparticle to the microorganism was studied in Pseudomonas aeruginosa and Bacillus subtilis through agar diffusion method, minimum inhibitory concentration, swarming motility assay and protein leakage assay. The nanoparticles were found to be more effective against the bacteria used in this study.
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