Evaluation of biochemical indicators in blood plasma of rats with tetracycline-induced hepatosis and their correction by milk phospholipids

Abstract

tetracycline is a drug with direct cytotoxic action on the liver, and therefore it is widely used in pharmaceutical studies of therapeutic effectiveness of hepatoprotective preparations. The aim of the present work was to determine the biochemical indicators in blood plasma of rats with tetracycline-induced hepatosis and correction properties of milk phospholipids under tetracycline-induced hepatosis in rats. to achieve this, Wistar rats were administered 250 mg/kg of 4% tetracycline hydrochloride suspension once a day intragastrically. As the corrective therapy, 1% solution of BAS "FlP-MD" was administered in liposomal form based on milk phospholipids. Under modeled steatohepatitis, significant destructive changes were observed in the cell membranes of hepatocytes in experimental rats. It was confirmed by higher activity of transaminase (in particular, activity of АSТ increased 4 times, that of ALT 1.7 times and the AST/ALT ratio was increased 2.4 times in blood plasma). the synthesis of clotting factors in livers of animals with hepatosis was inhibited. The content of fibrinogen in blood plasma decreased by 21%, factor II (prothrombin) by 27.8%, Xa-factor by 27.9%, and protein C by 40.6%. The animals also had hypochromic anemia, azotemia and bilirubinemia. the calcium-phosphor metabolism and hyperkalemia were observed. the liposomal BAS "FlP-MD" based on milk phospholipids diminished harmful effects of tetracycline, in particular supporting blood coagulation factors' level restoration, and also by the activity of transaminases. According to the results, it may be used in prophylactics and pharmaceutical correction of steatohepatitis.

Influence of Different Stocking Densities on Some Blood Parameters in Laying Hens

“…The increase in the content of leukocytes is a characteristic response of immunocompetent tissues to the action of glucocorticoids and catecholamines, the concentration of which in the blood of hens increases under the influence of various stressors (Jiang et al, 2017;Gryshchenko et al, 2019). Thus, the basis for the development of leukocytosis is an increase in the heterophil content in the blood, as noted in these studies (Table 5).…”

Blood coagulation factors and platelet response to drug‐induced hepatitis and hepatosis in rats

“…These findings confirmed the destructive changes in hepatocytes, decrease in protein synthesis, disruption of pigment metabolism, and signs of cholestasis during the development of experimental drug‐induced hepatosis modeling. 7 , 17 …”

“…These findings confirmed the destructive changes in hepatocytes, decrease in protein synthesis, disruption of pigment metabolism, and signs of cholestasis during the development of experimental drug-induced hepatosis modeling. 7,17 The development of the experimental drug-induced hepatitis 18,25 model provoked changes in hematological and protein profiles. Pronounced clinical symptoms of the disease began to appear on the sixth day of daily administration of diclofenac.…”

“…Liver injury leads to the disruption of protein synthesis 7,8 . Hepatocytes are the main source of clotting factors.…”

“…[4][5][6] Liver injury leads to the disruption of protein synthesis. 7,8 Hepatocytes are the main source of clotting factors. The disruption of liver functions leads to changes in the γ-carboxylation of vitamin K-dependent factors (mainly factors VII, IX, and X; prothrombin; and proteins С, S, and Z).…”