Evaluation of biofilm formation, cell surface hydrophobicity and gelatinase activity in Acinetobacter baumannii strains isolated from patients of diabetic and non-diabetic foot ulcer infections

Khan, Diwan Mahmood; Manzoor, Muhammed A.P.; Rao, I. Venkatakrishna; Moosabba, M.S.

doi:10.1016/j.bcab.2019.01.045

Cited by 9 publications

(2 citation statements)

References 35 publications

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“…Urease is an enzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide, thereby playing a key role in urea-related diseases such as urolithiasis [26]. Gelatinase is a protease that is capable of hydrolyzing gelatin, which may lead to tissue necrosis and damage to the body [27].…”

Section: Discussionmentioning

confidence: 99%

Selection and Characterization of Probiotic Bacteria Exhibiting Antiadipogenic Potential in 3T3-L1 Preadipocytes

Lee

Park²,

Kim

2021

Probiotics & Antimicro. Prot.

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Abnormal adipocyte growth, distinguished by an increase in cell numbers and cellular differentiation, is regarded as a major pathological characteristic of obesity. Thus, inhibition of adipogenic differentiation in adipocytes could prevent obesity. Recently, certain probiotic stains have been reported to regulate lipid metabolism in vitro and/or in vivo. In this backdrop, this study aimed to investigate basic probiotic properties and potential antiobesity characteristics of mouse 3T3-L1 preadipocytes. Six lactic acid bacteria (LAB) strains were prescreened for their cholesterol-lowering activity, antioxidant activity, and survival at low pH and in a solution containing bile salts. These six strains were investigated for antiadipogenic activity by employing 3T3-L1 mouse preadipocytes. 3T3-L1 cells were treated with selected strains during the differentiation process. Lactobacillus johnsonii 3121 and Lactobacillus rhamnosus 86 were found to be more capable of reducing triglyceride and lipid accumulation, as compared to control group, which are fully differentiated 3T3-L1 adipocytes. These strains also inhibited adipocyte differentiation by downregulating the adipogenic transcription factor in 3T3-L1 adipocytes. Taken together, these results indicate that L. johnsonni 3121 and L. rhamnosus 86 could potentially act as probiotic bacteria and prevent fat accumulation by regulating adipogenesis-related markers.

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Section: Discussionmentioning

confidence: 99%

Selection and Characterization of Probiotic Bacteria Exhibiting Antiadipogenic Potential in 3T3-L1 Preadipocytes

Lee

Park²,

Kim

2021

Probiotics & Antimicro. Prot.

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“…In some reports, tuberculosis was also been found to be associated with diabetes mellitus patients and in case of DFU 58 that provides a suitable environment for various opportunistic pathogen. So far, all the reports of DFU wound formation 66,67 and majority of the species isolated from wound region have shown multiple drug-resistance property. 68 Moderate to severe grade biofilm-forming bacteria are found to have a direct role in wound healing 69 and interaction of host system by bacteria, which play a major role in understanding how these opportunistic pathogen creates environment to overcome host immune response.…”

Section: Drug Resistance and Biofilm Formation Of Pseudomonas Speciesmentioning

confidence: 99%

Combinatorial Drug Therapy for Controlling Pseudomonas aeruginosa and Its Association With Chronic Condition of Diabetic Foot Ulcer

Srivastava

Karthikeyan

2019

The International Journal of Lower Extremity Wounds

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Diabetic foot ulcer (DFU) is a major complication of diabetes mellitus, major observations of DFU cases have reported on amputation of foot region, and microbial bioburden during DFU is a major cause that affects healing of the wound regions. Pathogenic microbes are routinely isolated from these wound regions, especially Staphylococcus, Pseudomonas, Klebsiella, and Escherichia coli have been reported, whereas higher prevalence of Pseudomonas species during chronic condition in the deeper part of the wound, when left untreated, leads to gangrene. Multiple drug-resistant Pseudomonas strains are a new threat because of their biofilm-forming ability, making it more potent and incurable. Acyl homoserine lactones (AHL) are a group of signaling molecules that can regulate biofilm growth, and Las and Rhl operon generally work in tandem to initiate biofilm formation by Pseudomonas species. These signaling molecules also initiate virulence factors that correlates upregulation of inflammatory responses, and AHL can be a therapeutic target in order to prevent the efficacy of multiple drug-resistant strains that form biofilm and also can be an alternative solution against control of multiple drug-resistant strains.

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Screening for Lactic Acid Bacterial Strains as Probiotics Exhibiting Anti-inflammatory and Antioxidative Characteristic Via Immune Modulation in HaCaT Cell

Shin

Lee

Kim

2023

Probiotics & Antimicro. Prot.

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In this study, the basic probiotic characteristics and functional properties of lactic acid bacteria (LAB) were investigated using two in vitro models of in ammation induced by lipopolysaccharide (LPS) and H 2 O 2 . Fifteen strains were prescreened out of 60 LAB candidates based on their radical scavenging activity to determine the antioxidant capacity of the strains. The top 15 candidates were further investigated to evaluate their survival rate under low pH and bile salt conditions that mimic the intestinal environment. Three strains, Lactobacillus brevis D70, Lactobacillus pentosus S16, and Lactobacillus fermentum MF10, were capable of scavenging free radicals and survived under arti cial intestinal conditions. Therefore, Lact. brevis D70, Lact. pentosus S16, and Lact. fermentum MF10 were selected for further antioxidant, anti-in ammation, and mitochondrial activity examinations via cell models of in ammation and oxidative stress) Among the three strains, Lact. fermentum MF10 showed the highest anti-in ammatory and antioxidative activities by downregulating the relative mRNA expression levels of in ammatory biomarkers induced by LPS and upregulating the gene expression levels of antioxidative mediators induced by reactive oxygen species (ROS) in both human HT-29 epithelial cells and human HaCaT keratinocytes. Lactobacillus fermentum MF10 was also capable of regulating mitochondrial membrane potential (MMP), which plays a key role in the mitochondrial activity of HaCaT cells. As a result, Lact. fermentum MF10 showed the highest potential for probiotic properties and impacts the immune-related gut-skin axis by altering proin ammatory cytokines, antioxidative biomarkers, and MMP.

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Evaluation of biofilm formation, cell surface hydrophobicity and gelatinase activity in Acinetobacter baumannii strains isolated from patients of diabetic and non-diabetic foot ulcer infections

Cited by 9 publications

References 35 publications

Selection and Characterization of Probiotic Bacteria Exhibiting Antiadipogenic Potential in 3T3-L1 Preadipocytes

Selection and Characterization of Probiotic Bacteria Exhibiting Antiadipogenic Potential in 3T3-L1 Preadipocytes

Combinatorial Drug Therapy for Controlling Pseudomonas aeruginosa and Its Association With Chronic Condition of Diabetic Foot Ulcer

Screening for Lactic Acid Bacterial Strains as Probiotics Exhibiting Anti-inflammatory and Antioxidative Characteristic Via Immune Modulation in HaCaT Cell

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