Evaluation of biofilm production by Pseudomonas Aeruginosa isolates recovered from cystic fibrosis and non-cystic fibrosis patients

Costa, Maurício Dutra; Freitas,; Barth,

doi:10.1590/s1517-83822011000200011

Cited by 39 publications

(46 citation statements)

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“…Takođe, u radu je prikazan relativan odnos izmerenih vrednosti apsorbanci u odnosu na standardni biofilm-neprodukujući soj P. aeruginosa ATCC 27853 i izvršena klasifikacija izolata Pseudomonas aeruginosa na biofilm-neprodukujuće, slaboprodukujuće, srednjeprodukujuće i visokoprodukujuće sojeve [10] na sledeći način:…”

Section: Eksperimentalni Deounclassified

“…Rezultati ovih istraživanja ukazuju da izolati P. aeruginosa, posebno oni koji potiču iz različitih životnih sredina, pokazuju i razlike u sposobnosti formiranja biofilmova. Međutim, opisano je i oko 2% sojeva koji nemaju ovu sposobnost [10].…”

Section: Eksperimentalni Deounclassified

“…Rezultati ispitivanja drugih autora [10] ukazuju na sličan odnos među kliničkim izolatima P. Aeruginosa. Od 74 ispitana izolata, 68 % je okarakterisano kao slabo-produkujući, a samo su dva izolata bila okarakterisna kao srednje-produkujući.…”

Section: Grafikon 1 -Mogućnost Formiranja Biofilmova Izolata P Aerugunclassified

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Potential of pseudomonas aeruginosa environmental isolates for biofilm formation

Vujović¹,

Rudić²,

Igor³

et al. 2016

Zas Mat

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Section: Eksperimentalni Deounclassified

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Potential of pseudomonas aeruginosa environmental isolates for biofilm formation

Vujović¹,

Rudić²,

Igor³

et al. 2016

Zas Mat

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“…11 Three to five colonies were suspended in 5 ml of Trypticase soy broth (TSB) and incubated for 24 hrs. Then, diluted and adjusted to 0.5 McFarland turbidity standards to reach 10 5 CFU/ml.…”

Section: Quantitative Assaymentioning

confidence: 99%

“…The absorbance value (OD) of the test organism is interpreted with the criteria given below 11,12 depending on standard calculations laid down by by which the study isolates were classified. 7 An optical density of 0.0541 was chosen as guideline to distinguish biofilm producers from the non-biofilm producers.…”

Section: Quantitative Assaymentioning

confidence: 99%

The effectiveness of antibiotics against a major uropathogen- Escherichia coli and its biofilm assay by phenotypic methods

2016

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INTRODUCTIONUrinary tract infection (UTI), an inflammatory disease occurs due to high multiplication of pathogenic microbes in the urinary system, altering the perfect function of urinary tract and kidney. The bacterial UTI is the second most serious health problem and causing morbidity. 1About 50% -80% of population endures UTI in their lifetime; 20% -50% will have recrudescent infections. 2Globally 150 million people are UTI diagnosed each year and $6 billion high expenditure in all age groups. This infection focuses towards young, sexually active women than men, though the prevalence in elderly men and women are similar. A study conducted in US has estimated that around 40% -50% of healthy adult women will experience this infection. 3Escherichia coli commensal of the colon via symbiotic relationship causes intestinal infection opportunisticaly and a common cause of extra intestinal infection as UTI accounting for up to 80% of community acquired UTI's making it to consider as a major uropathogen. E.coli has ABSTRACT Background: Urinary tract infection (UTI), an inflammatory disease occurs to a high multiplication of pathogenic microbes in the urinary system. Escherichia coli, a major uropathogen accounting for up to 80% UTI. Activity of antibiotics against E.coli is decreasing due to enzymatic, genetic and various other factors resulting in multidrug resistance. Biofilm is a great threat as it interferes antibiotic therapy, undergoes gene transformation, making the bacteria more virulent. Objectives of the study were to isolate the E. coli from the urine sample of symptomatic UTI patients, to study the effectiveness of antibiotics against E.coli by antibiotic susceptibility pattern, ESBL detection and to biofilm phenotype assay by four different methods. Methods: A total of 400 urine samples from symptomatic UTI patients, October 2015 to March 2016 and processed. E.coli was isolated and antibiotic susceptibility was done as per CLSI guidelines. Biofilm detection by a) Congo red agar (CRA), b) Modified Congo red agar (MCRA), c) Tube adherence assay, d) Microtitre plate method. Results: A total of 100 E. coli (69%) was obtained from 400 samples, 49% were ESBL producers and 84% shows multi drug resistant. Biofilm positive isolates shows 70% positivity by CRA, 91% by MCRA, 57% in tube adherence assay and 99% via microtitre plate method. Conclusions: Resistance to antibiotics ladder is increasing and it's necessary to take actions to reduce its hindrance in the future. Advanced studies in biofilm will help to prevent the more virulence without any critical complications in therapy.

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Bacteria and Biofilms in Chronic Infections and Nosocomial Diseases

Pattnaik

2021

Biofilm-Mediated Diseases: Causes and Controls

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Evaluation of biofilm production by Pseudomonas Aeruginosa isolates recovered from cystic fibrosis and non-cystic fibrosis patients

Cited by 39 publications

References 10 publications

Potential of pseudomonas aeruginosa environmental isolates for biofilm formation

Potential of pseudomonas aeruginosa environmental isolates for biofilm formation

The effectiveness of antibiotics against a major uropathogen- Escherichia coli and its biofilm assay by phenotypic methods

Bacteria and Biofilms in Chronic Infections and Nosocomial Diseases

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