Evaluation of biological behavior of odontogenic keratocyst and orthokeratinized odontogenic cyst using picrosirius red stain: A clinicopathological retrospective study

Manthapuri, Suvarna; Shylaja, S.; Mantha, Harshini; Oruganti, Ramanand V; Reddy, Sharath Kumar; Vamshi, V Raghu

doi:10.4103/jdrntruhs.jdrntruhs_69_19

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…In this research, collagen III revealed expression in all OKCs cystic wall, which supports the finding of other studies 30,31 and might indicate the tumor behavior of this cyst.…”

Section: Discussionsupporting

confidence: 91%

Impact of Immunohistochemical Expression of Collagen III and MMP-14 in Odontogenic Keratocyst

Hassan,

Garib,

Mohammad

2023

SDJ

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Objective: Immunohistochemical expression of collagen III and MMP14 was evaluated in odontogenic keratocyst, and correlated tothe clinicopathological parameters to remark on the role of these markers in the biological behavior of the cyst.Methods: Twenty-three odontogenic keratocysts samples were collected from three pathological laboratories in Sulaimani.Demographic information and the available radiographic investigation were recorded. The immune expression of collagen III andMMP14 was evaluated and related to the clinicopathological variables. Chi-square test was used for analysis and a p-value of 0.05 wascut off point for significance.Results: Males were predominantly affected by odontogenic keratocysts lesions (69.6%), which were mainly detected in the >35 yearsage groups. Keratocyst lesions were seen more frequently in the mandible (82.6%), with well-defined borders, and unilocularappearance. Collagen III was oriented more commonly in a parallel direction (70.8%), with moderate intensity (45.8%). MMP14revealed cytoplasmic expression in the epithelial lining of the keratocyst (100%), score 3 was the most prevalent expression (54.2%)in the cystic wall, and prominent inflammation and epithelial separation were detected with high scoring of MMP14, which were(57.1%) and (56.3%) respectively.Conclusions: Both markers had independent combined roles in the cyst’s biological behavior, including the prevalent parallel direction of the collagen III, while overexpression of the MMP14 might have an impact role in the aggressive behavior of the lesion.

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“…In this research, collagen III revealed expression in all OKCs cystic wall, which supports the finding of other studies 30,31 and might indicate the tumor behavior of this cyst.…”

Section: Discussionsupporting

confidence: 91%

Impact of Immunohistochemical Expression of Collagen III and MMP-14 in Odontogenic Keratocyst

Hassan,

Garib,

Mohammad

2023

SDJ

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“…6 In the present study, it was observed that twelve cases (60%) of OKCs exhibited a predominance of green-yellow birefringence, this was in accordance with other several studies. 5,[10][11][12][13] Green-yellow polarization is related to loosely packed collagen fibers, this is in agreement with Sharf et al 14 who advocated that, physical aggregation of collagen molecules determine the polarization color; therefore, it was found that loosely packed collagens represented as greenyellow, this is also in conjunction with Junqueira et al, and Montes et al 12 who documented that, loose thin fibrils of type III collagen are found to physically form a loose meshwork in the ground substance which in turn exhibit a weak birefringence of greenyellow. 15 It was also stated that, green-yellow color implies that stromal fibers consist of pro-collagen, intermediate, or pathologic collagens with disorganized pattern, such fibers are not structurally stable; hence, denature easily by the action of proteolytic enzymes of the stromal tissue.…”

Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of Polarization Colors of Collagen Fibers in Odontogenic Keratocyst and Ameloblastoma Using Picrosirius Red Stain (Histochemical Study)

M.O Bin Hussain,

A. Sultan

2021

Libyan J Dent

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Aims: studying the quality and quantity of collagen fibers may help to delineate their influence on the aggressiveness and growth potential of the OKC and ameloblastoma. The aims of this study were to evaluate and compare the polarization colors, orientation, and organization of collagen fibers in the connective tissues of the odontogenic keratocyst (OKC), and ameloblastoma (AB) using picrosirius red (PSR) stain under a polarizing microscope with correlation to their biologic behavior. Materials and method: polarization colors, orientation, and organization of collagen fibers in pre-diagnosed cases of OKC and AB, twenty each, and two control cases of dentigerous cyst (DC) were histochemically analyzed using PSR stain and a polarized light microscope. Results: comparing the collagen fibers in the two lesions, there was no significant difference with respect to the polarization colors, orientation, and organization. Conclusion: PSR staining and polarizing microscopy is a powerful tool to appraise the nature and arrangement of collagen fibers in odontogenic lesions. The connective tissue of OKC showed a predominance of green-yellow birefringence, and loosely packed fibers, this can be correlated to the aggressive nature of this lesion.

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Incidental finding of orthokeratinized odontogenic cyst with unusual features

Nandini

Devi

Deepak

et al. 2022

Journal of Oral and Maxillofacial Pathology

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Orthokeratinized odontogenic cyst (OOC) is a rare developmental odontogenic cyst occurring in the jaw with debated etiology. It was originally believed to be a variant of odontogenic keratocyst (OKC) but is now considered to be a distinct entity. The majority of the cases occur in the third and fourth decades of life. The common site is the mandibular posterior region with a male predilection. Swelling is the most common symptom which may be accompanied by pain, although in most cases, the lesion is asymptomatic. These lesions mostly present as unilocular radiolucency often associated with an impacted tooth. They may mimic dentigerous cyst and OKC in radiologic and histopathologic presentation, however, differ in biological behavior, pathogenesis and prognosis in comparison. Hence, making an accurate diagnosis is essential. This article describes an incidental finding of OOC in a 28-year-old female during radiographic investigation for orthodontic treatment. This case showed some rare features such as multilocular radiolucency, nonkeratinized epithelium in areas of inflammation, few cholesterol clefts with giant cells, presence of dentinoid-like material and dystrophic calcification in the capsule.

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Evaluation of biological behavior of odontogenic keratocyst and orthokeratinized odontogenic cyst using picrosirius red stain: A clinicopathological retrospective study

Cited by 3 publications

References 17 publications

Impact of Immunohistochemical Expression of Collagen III and MMP-14 in Odontogenic Keratocyst

Impact of Immunohistochemical Expression of Collagen III and MMP-14 in Odontogenic Keratocyst

Comparative Evaluation of Polarization Colors of Collagen Fibers in Odontogenic Keratocyst and Ameloblastoma Using Picrosirius Red Stain (Histochemical Study)

Incidental finding of orthokeratinized odontogenic cyst with unusual features

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