Evaluation of blast resistance genes Pi9 and Pi54 in rice against local isolates of Tamil Nadu

Abstract

Rice production system is greatly affected by blast disease caused by Magnaporthe grisea Barr which causes significant yield reduction throughout Asia and different parts of the globe. Developing resistant varieties has been most efficient and economical method for controlling blast disease in rice. Identification of broad spectrum resistance genes against prevailing isolates of the pathogen is a key determining success of developing resistant varieties. However, the blast resistance characterization and evaluation has not been well studied. In the current study, 3 parental lines namely CO 51 (Pi54), 562-4 (a NIL of CO 43 harboring Pi 9) and a susceptible genotype Improved White Ponni (IWP) were evaluated against local isolates of blast pathogen. Presence of Pi9 and Pi54 blast resistance genes in the parental lines was confirmed by using functional markers such as NBS4 and Pi54MAS respectively. All the three genotypes were evaluated against blast disease in a hot spot environment. Both CO51 and 562-4 carrying the Pi54 and Pi9 genes respectively showed moderate resistance against blast disease whereas IWP showed a susceptibility reaction against blast disease. Results indicated that pyramiding of these genes would improve resistance against blast disease. The present study has favored the selection of blast resistant parental lines which can be utilized as donor in breeding programs.

Conventional Breeding and Molecular Markers for Blast Disease Resistance in Rice (Oryza sativa L.)

“…This means that this gene may have no effect under the Egyptian conditions or there are any other gene(s) is affecting the expiration of this gene. Similar results were obtained with previous studies [44][45][46][47]. Positive amplified fragments for YL153/YL154 marker that linked to Pita-Pita-2 gene were detected in three Egyptian genotypes (Sakha 105, Sakha 101, and Sakha 104) and IRBLTA-CP1 monogenic line.…”

Blast resistance (Pi54) introgression in temperate rice (Oryza sativa L.) K343 using marker assisted backcrossing

“…The amplified product was separated using 3-3.5% MetaphorTM Agarose gel electrophoresis, visualized with an ultraviolet transilluminator, and photographed with a gel documentation unit (MiniLumi by DNR Bio-Imaging System, Israel). Three SSR markers closely linked to Pi54, i.e., TRS26 and TRS33 (flanking markers for Pi54 gene with a distance of 0.7 and 0.5 cM, respectively) and RM206 (0.7 cM), were selected based on earlier studies (Dai et al, 2018;Fjellstrom et al, 2006;Kumari et al, 2013;Rani and Adilakshmi, 2011;Sharma et al, 2005;Thulasinathan et al, 2020;Vasudevan et al, 2015;Vijay Kumar et al, 2018). All markers selected were individually screened for resolution on 3 percent agarose gel.…”

“…The Pi54 gene, among these, offers broadspectrum resistance to blast fungus and is very effective against the local blast pathogen. This gene was cloned and is located on rice's chromosome 11L (Kumari et al, 2013;Sharma et al, 2005;Thulasinathan et al, 2020;Vasudevan et al, 2015).…”

Improving rice blast resistance by stacking of two broad spectrum resistance gene Pi9 and Pi54 in cultivar CO 51 through Marker Assisted Selection

“…Parent materials: In the present study, the CO51 cultivar, which is a short-duration (110-115 days), fine-grain, and high-yielding rice genotype, was notified for cultivation in 14 different states in India. CO51, being the recurrent parent, exhibits moderate resistance to blast disease due to the presence of the Pi54 resistant allele on chromosome 11 at 24.2 Mb (Ashkani et al, 2016;Devanna et al, 2022;Thulasinathan et al, 2020). The aim of this study is to enhance the resistance of CO51 against the blast pathogen by introducing the Pi9 gene along with to the Pi54 allele.…”

“…Gene, Pi54 exhibits resistance against major M. oryzae isolates (Rai et al, 2011;Dixit et al, 2020). Both Pi9 and Pi54 have durable and broad-spectrum resistance against the blast pathogen M. Oryzae (Kumar et al, 2018;Thulasinathan et al, 2020). Therefore, the stacking or pyramiding of Pi9 and Pi54 genes may offer broad-spectrum and durable resistance against a wide range of blast pathogen races.…”