Evaluation of blood, buccal swabs, and hair follicles for DNA profiling technique using STR markers

Chaudhary, Garima; Dogra, Tirath Das; Raina, Anupuma

doi:10.3325/cmj.2015.56.239

Cited by 11 publications

(4 citation statements)

References 25 publications

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“…For hair follicle samples, no donor chimerism has been observed; thus, they can be used as a reliable biological source for personal identification by DNA profiling. Great care must be taken to avoid possible contamination while collecting the samples (17). It has been shown that peripheral blood DNA profiles of recipients show donor chimerism after successful allo-PBSCT.…”

Section: Discussionmentioning

confidence: 99%

DNA profiling in blood, buccal swabs, and hair follicles of transplantation patients

Zeybek

Arisoy

2016

Turk J Med Sci

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Background/aim: Peripheral blood DNA profiles are often used in forensic identification and paternity investigations. Currently, because of the increasing number of patients who undergo transplantation, determining the history of transplantation has gained importance in forensic cases. The aim of this study was to investigate the potential of genetic chimerism analysis of blood, hair follicle, and buccal swab samples for the forensic identification of transplant patients. Materials and methods: Blood, hair follicle, and buccal swab samples from five patients who had undergone allogeneic peripheral blood stem cell transplantation (allo-PBSCT) and 35 patients who had undergone liver transplantation were analyzed. After isolation of DNA from the samples, specific short tandem repeat sequences were amplified using polymerase chain reaction and genotype was determined using capillary electrophoresis. Results: DNA profiles of the patients were compared. The DNA profiles of blood, hair follicle, and buccal swab samples of four patients who had undergone allo-PBSCT were completely different; there was no mixed chimerism. Conclusion: It is known that after successful allo-PBSCT, DNA profiles of peripheral blood samples show donor chimerism, those of buccal swab samples vary with duration after transplantation, and those of hair follicle samples remain unchanged. Our results were in agreement with these findings.

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Section: Discussionmentioning

confidence: 99%

DNA profiling in blood, buccal swabs, and hair follicles of transplantation patients

Zeybek

Arisoy

2016

Turk J Med Sci

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“…E very nucleated cell contains deoxyribonucleic acid (DNA) and analysis of this genome gives insight to the individuals' unique genetic identity and potential risk status for falling ill with genetic diseases (Sugata et al, 2002;Chaudhary et al, 2015;Fiorani and Cinotti, 2020). In the clinical sector, predictive screening, diagnostic testing for certain genotypes, possible somatic or germline mutations, biomarkers, and epigenetic anomalies can provide insight about a patient's predisposition for a certain disease or individual tolerance of drugs and treatments in the field of personalized medicine (Marteau and Croyle, 1998;Rahner and Steinke, 2008;Jostins and Barrett, 2011;Roberts et al 2012;Whirl-Carrillo et al, 2012;Cascella et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Maximum DNA Yield from a New Noninvasive Buccal Collection Device Following Various Extraction Protocols

Pißarreck

Moreira

Foley

2021

Genetic Testing and Molecular Biomarkers

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Background: As the demand in genetic testing increases, various fields look toward collection methods that are noninvasive and efficient in recovering deoxyribonucleic acid (DNA) for testing that will allow for high firstpass success rates. Objective: Two extraction methods (PrepFilerÔ Express Forensic Extraction and the Maxwell Ò RSC Buccal Swab DNA Kits) were optimized to increase DNA yield from a buccal cell collection device (Gentueri's CollectEjectÔ Swab). Materials and Methods: Buccal swabs were processed under varying incubation parameters using a forensic workflow. The PrepFiler method was adjusted to test longer incubation times and more aggressive agitation. The Maxwell method was adjusted to test incubation temperatures and duration. Results: Quantitative results showed that increased agitation can yield more DNA through the PrepFiler extraction, but longer incubation times did not increase DNA recovery. The results from the Maxwell study showed no significant difference between incubation temperatures or times. Conclusions:The results indicate that various applied genetic fields can utilize a noninvasive, simple collection method using the CollectEject device in conjunction with extraction methods already implemented in laboratories to collect 5000 ng of DNA or greater from a buccal cell collection.

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“…Chimerism is defined in transplantation medicine as the coexistence ofcells of, both, donor and recipient origin (Ten Hove et al, 2007). In previous studies, blood, hair samples and buccal swabs are studied as sources of DNA profiling after hematopoietic stem cell transplantation (Hong et al, 2007;Zhou et al, 2011;Li et al, 2014;Chaudhary et al, 2015;Santurtún et al, 2017) The partial chimerism is detectable predominantly in the P hematopoietic system developed by transfusion or transplantation of allogenic hematopoietic stem cells (Milde et al, 1999). This experimental work aims at studying the possibility of postmortem detection in DNA chimerism in liver tissue, after allogeneic hematopoietic stem cell transplantation.…”

Section: Introductionmentioning

confidence: 99%

Postmortem Detection of Liver Chimerism after Allogeneic Hematopoietic Stem Cell Transplantation in Adult Male Mice

Wahab

Allah

Ibrahim

et al. 2018

Zagazig Journal of Forensic Medicine

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Introduction: Postmortem personal identification is based on the extraction of deoxyribonucleic acid (DNA) from human remains or different human materials. There are some reports proving that various tissues may differ genetically and do not always have the DNA profile of the person from whom they originate due to mutation or chimerism. Aim of the work: The work aimed at the postmortem detection of the possible change in DNA fingerprint after allogeneic hematopoietic stem cell transplantation in liver tissue samples. Materials and Methods: One hundred twenty eight adult male mice weighing 25g. DNA fingerprint first was done from blood samples. After 3 months from interference, postmortem DNA fingerprinting was done again from liver tissue. Results: Fluorescent stem cells were detected postmortem in liver tissue of 30 mice by percentage of 93.75%. The change in DNA profile was detected postmortem in 20 mice in liver tissue samples by a percentage of 62.5%. Conclusion: DNA chimerism, after allogeneic hematopoietic stem cell transplantation, can be detected postmortem in the recipient's liver. Recommendations: It's recommended to study the postmortem detection of DNA chimerism in organs other than the liver to compare the percentage of chimerism detection.

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Evaluation of blood, buccal swabs, and hair follicles for DNA profiling technique using STR markers

Cited by 11 publications

References 25 publications

DNA profiling in blood, buccal swabs, and hair follicles of transplantation patients

DNA profiling in blood, buccal swabs, and hair follicles of transplantation patients

Evaluation of Maximum DNA Yield from a New Noninvasive Buccal Collection Device Following Various Extraction Protocols

Postmortem Detection of Liver Chimerism after Allogeneic Hematopoietic Stem Cell Transplantation in Adult Male Mice

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