Evaluation of Cameroonian plants towards experimental bone regeneration

Ngueguim, Florence Tsofack; Khan, Mohd Parvez; Donfack, Jean Hubert; Siddiqui, Jawed A.; Tewari, Deepshikha; Nagar, Geet Kumar; Tiwari, Satish C.; Dimo, Théophile; Maurya, Rakesh; Chattopadhyay, Naibedya

doi:10.1016/j.jep.2012.02.041

Cited by 27 publications

(21 citation statements)

References 19 publications

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“…A drill-hole injury was created in animals as previously described [4, 9, 10]. Briefly, the front skin of the mid femur in rats was incised straight and longitudinally at 1 cm in length under anesthesia.…”

Section: Drill Hole Injury In the Femurmentioning

confidence: 99%

“…Generally in modern medicine, treatment of bone fractures involve the use of a cast or plaster to immobilise the fractured limb, followed by the administration of analgesics (pain killers) and anti-inflammatory drugs to patients. The broken bone is then allowed to regenerate naturally on its own [3], since there is no orally active pharmacological agent available for rapid fracture repair [4]. Even though fractures heal naturally, it would be of great interest to discover alternatives to accelerate a bone healing process, thereby reducing the healing period, reducing the cost of treatment and helping patients to quickly regain their good health.…”

Section: Introductionmentioning

confidence: 99%

“…Literature data confirms the hypoglycemic, anti-inflammatory and analgesic effects of P. pellucida [7] and equally its antidiabetic [8] properties. Several animal models are used to show bone regeneration among which is drill-hole injury model [4, 9, 10]. We recently demonstrated that, the ethanolic extract of P. pellucida promotes fracture healing in rat by anabolic effects on osteoblasts using drill-hole injury model [10].…”

Section: Introductionmentioning

confidence: 99%

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Aqueous extract of Peperomia pellucida (L.) HBK accelerates fracture healing in Wistar rats

Florence

Huguette

Hubert

et al. 2017

BMC Complement Altern Med

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Background Peperomia pellucida (L.) HBK is consumed as vegetable and used in Cameroonian traditional medicine for the management of diseases and for fracture healing. Therefore the aim of this study was to evaluate the effects of the aqueous whole plant extract of Peperomia pellucida on fracture healing in female Wistar rats.MethodsA drill hole injury was created by inserting a drill bit inthe diaphysis of the femur. The aqueous extract of the whole plant of Peperomia pellucida was administered orally at the doses of 100, 200 and 400 mg/kg to adult female Wistar rats. The vehicle (distilled water) was given to the control. Besides these rats, one group of rats without fracture received the extract (400 mg/kg). After 14 days of treatment, the rats were sacrificed under anesthesia and the effects of the extract were evaluated on body weight, the relative weights of organs (femurs, uteri and ovaries) and on hematology. Bone (calcium, phosphorus, alkaline phosphatase) and serum biochemical parameters (calcium, phosphorus, alkaline phosphatase) were also evaluated. Radiological and histological tests were carried out on the femurs. The mineral content of the plant extract was also investigated.ResultsThe extract induced an increase in body weight at high dose and in WBCs count at low doses. Aqueous extract from Peperomia pellucida increased bone calcium at lowest dose but maintained this parameter at normal range at high dose in fractured rat. Alkaline phophatase and phosphorus concentrations reduced significantly (p < 0.01) at the dose of 400 mg/kg as compared to fractured rats. Moreover, radiological tests revealed a dose dependent formation of callus at the level of the fracture gap, confirmed by the formation of a highly dense and compact fibrocartilagenous callus. The mineral content of the plant extract revealed the presence of calcium, phosphorus, magnesium, sodium and potassium.ConclusionThe aqueous extract of P. pellucida accelerates bone healing due partly to the mineral content of the extract. These results confirm its traditional use in the treatment of bone fractures.

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Section: Drill Hole Injury In the Femurmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aqueous extract of Peperomia pellucida (L.) HBK accelerates fracture healing in Wistar rats

Florence

Huguette

Hubert

et al. 2017

BMC Complement Altern Med

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“…A drill-hole injury was created by inserting a drill bit with a diameter of 0.8 mm in the femur metaphysis region following our previously described protocol with minor modification with respect to drill-hole spot [30]. On the fourteenth day, rats were killed and bones appropriately preserved for histology, histomorphometry and micro-computed tomography (μCT) following our previously described methods [31]. Twenty-four hours before killing, all animals received calcein (20 mg/kg) by intraperitonial route.…”

Section: Msc Differentiation To Osteoblasts and Adipocytesmentioning

confidence: 99%

Ovariectomized Rats with Established Osteopenia have Diminished Mesenchymal Stem Cells in the Bone Marrow and Impaired Homing, Osteoinduction and Bone Regeneration at the Fracture Site

Tewari

Khan

Sagar

et al. 2014

Stem Cell Rev and Rep

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We investigated deleterious changes that take place in mesenchymal stem cells (MSC) and its fracture healing competence in ovariectomy (Ovx)-induced osteopenia. MSC from bone marrow (BM) of ovary intact (control) and Ovx rats was isolated. (99m)Tc-HMPAO (Technitium hexamethylpropylene amine oxime) labeled MSC was systemically transplanted to rats and fracture tropism assessed by SPECT/CT. PKH26 labeled MSC (PKH26-MSC) was bound in scaffold and applied to fracture site (drill-hole in femur metaphysis). Osteoinduction was quantified by calcein binding and microcomputed tomography. Estrogen receptor (ER) antagonist, fulvestrant was used to determine ER dependence of osteo-induction by MSC. BM-MSC number was strikingly reduced and doubling time increased in Ovx rats compared to control. SPECT/CT showed reduced localization of (99m)Tc-HMPAO labeled MSC to the fracture site, 3 h post-transplantation in Ovx rats as compared with controls. Post-transplantation, Ovx MSC labeled with PKH26 (Ovx PKH26-MSC) localized less to fracture site than control PKH26-MSC. Transplantation of either control or Ovx MSC enhanced calcein binding and bone volume at the callus of control rats over placebo group however Ovx MSC had lower efficacy than control MSC. Fulvestrant blocked osteoinduction by control MSC. When scaffold bound MSC was applied to fracture, osteoinduction by Ovx PKH26-MSC was less than control PKH26-MSC. In Ovx rats, control MSC/E2 treatment but not Ovx MSC showed osteoinduction. Regenerated bone was irregularly deposited in Ovx MSC group. In conclusion, Ovx is associated with diminished BM-MSC number and its growth, and Ovx MSC displays impaired engraftment to fracture and osteoinduction besides disordered bone regeneration.

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“…Trabecular bone volume (BV/TV; percentage) and trabecular number (Tb.N) of femur epiphysis and proximal tibial metaphysis were calculated by the mean intercept length method. Trabecular thickness (Tb.Th; mm) was calculated according to the method of Hildebrand and Ruegsegger (Ngueguim et al, 2012). 3D parameters were based on analysis of a Marching cubes-type model with a rendered surface.…”

Section: Microcomputed Tomographymentioning

confidence: 99%

Effect of KH-BaRoKer-SeongJangTang based on traditional medicine theory on longitudinal bone growth

Kim¹,

Jeong²,

Park³

et al. 2014

TANG [HUMANITAS MEDICINE]

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KH-BaRoKer-SeongJangTang (KBS) is a recently developed formulation by using traditional drugs considering traditional medical theory of Oriental books such as ShinNongBonChoGyeong and JuRye, which has been used to improve the growth of child in Korea. Although KBS is usually prescribed to many children who are in retard for their age, its pharmacological effects have not been fully understood in experimental models. The aim of this study was to evaluate the effects of KBS on bone growth. Growth plate thickness and bone parameters such as bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), connection density (Conn.D), and total porosity were analyzed by means of microcomputed tomography. Serum insulin-like growth factor-I (IGF-I) levels were measured by enzyme-linked immunosorbent assay. Hepatic IGF-I mRNA expression was analyzed by real-time polymerase chain reaction. Phosphorylation of signal transducer and activator of transcription5 (STAT5) was investigated using Western blot analysis and immunohistochemistry. The thickness of growth plate was increased by KBS. BV/TV, Tb.Th, TbN, Conn.D, and total porosity were improved by KBS. Hepatic IGF-I mRNA and serum IGF-I levels were elevated by KBS. Phosphorylation of STAT5 was increased with administration of KBS. These results suggest that KBS would be helpful to children who are in retard for their age through the elevation of IGF-I.

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Evaluation of Cameroonian plants towards experimental bone regeneration

Cited by 27 publications

References 19 publications

Aqueous extract of Peperomia pellucida (L.) HBK accelerates fracture healing in Wistar rats

Aqueous extract of Peperomia pellucida (L.) HBK accelerates fracture healing in Wistar rats

Ovariectomized Rats with Established Osteopenia have Diminished Mesenchymal Stem Cells in the Bone Marrow and Impaired Homing, Osteoinduction and Bone Regeneration at the Fracture Site

Effect of KH-BaRoKer-SeongJangTang based on traditional medicine theory on longitudinal bone growth

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