Evaluation of candidate genotype of leptin gene associated with fertility and production traits in Hardhenu (Bos taurus × Bos indicus) cattle

Abstract

The present study was conducted on Hardhenu cattle to screen genomic region of leptin gene with an objective to find the association of genotypes with fertility and production traits. The association analysis with traits under study was analysed by least squares analysis of variance by taking SNPs genotype as fixed effects in the statistical model. The genotypic frequencies with respect to targeted loci g.92450765 G > A indicated that AG (0.54) genotype was highest in Hardhenu cattle. Chi-squared tests showed that g.92450765G > A SNP meet with the Hardy-Weinberg equilibrium (p > .05).The association analysis revealed significant association of genotypes with total milk yield (TMY) and 305 days milk yield (MY) (p < .05). Service period (SP) and calving interval (CI) were also found significantly associated with genotypes (p < .05). Whereas, lactation length (LL), dry days (DD) and age at first calving (AFC) did not divulge any significant association with genotype. The AG and GG genotypes were associated with higher milk yields as compared to AA genotype, indicating that allele G was associated with superior milk performance. However, AA genotyped cattle found to be favourable with SP, CI and artificial insemination (AI) per conception compared to AG and GG genotyped cows. Chi-square analysis revealed that genetic variants of g.92450765 G > A SNP of leptin gene differ significantly with regard to reproductive disorders incidence (p < .05). The frequency of GG genotype (88.89%) in the affected animal group was very high followed by AG. The animals with GG genotype were found to be more susceptible to reproductive disorders as suggested by the higher odd ratio value (16.00) in logistic model. These observations and their differential association with the fertility and production traits can be utilized as an aid to selection for genetic improvement of antagonistic traits in dairy cows.

Diversity of partial sequence leptin gene (Exon 3) in crossbred cattle compared to GenBank database

“…Matumoto et al ( 2022) reported that SNP c.239C>T (p.A80V) in leptin gene had significant effects on the traits related with quality grade; beef marbling standard (p: 0.0132), meat brightness (p: 0.0383), and meat firmness (p: 0.0115). Yadav et al (2020) reported that SNP has contribution to estrus and physical condition scores. The leptin gene has been reported to affect parenting each year (D'occhio et al 2019).…”

Identification of Polymorphism in LEP Gene by Single Strand Conformation Polymorphism (SSCP) in Rathi Cattle

“…They found two alleles and three genotypes in all four cattle breeds. Yadav et al, (2020) analyzed the genomic region of the leptin gene and found an association of production traits with all three genotypic patterns-AA, AG and GG in Hardhenu cattle. 3).…”

Association of CXCR1 gene polymorphism with clinical mastitis, reproductive disorders and performance traits in Hardhenu (Bos taurus × Bos indicus) cattle

“…The low productivity of indigenous animals and production and reproduction‐related diseases, such as mastitis, metritis and retention of placenta and dystocia in high‐yielding cattle are major concerns for the dairy industry. The susceptibility/resistance to mastitis and reproductive disorders is a threshold trait which is having low heritability (Falconer, 1965; Magotra et al, 2020; Yadav et al, 2020). Mastitis and reproductive disorders are among the most prevalent and economically detrimental health issues in dairy (García‐Sánchez et al, 2018; Kamaldeep et al, 2021; Krishnamoorthy et al, 2021; Rupp & Boichard, 1999).…”

“…trait which is having low heritability (Falconer, 1965;Magotra et al, 2020;Yadav et al, 2020). Mastitis and reproductive disorders are among the most prevalent and economically detrimental health issues in dairy (García-Sánchez et al, 2018;Kamaldeep et al, 2021;Krishnamoorthy et al, 2021;Rupp & Boichard, 1999).…”