Evaluation of canine red blood cell quality after processing with an automated cell salvage device

Hofbauer, Nina; Windberger, Ursula; Schwendenwein, Ilse; Tichy, Alexander; Eberspächer, Eva

doi:10.1111/vec.12472

Cited by 10 publications

(10 citation statements)

References 41 publications

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“…A similar finding was demonstrated with a cell salvage device that also used 0.9% NaCl to wash canine erythrocytes. 46 Careful handling of these units, including using the least number of washes, would reduce risk of in vitro hemolysis. Our study also showed an increase in total Hgb between the final wash and the post‐transfusion temperature samples.…”

Section: Discussionmentioning

confidence: 99%

“…The loss of central pallor, increased RDW and MCV, and decreased MCHC with washing supports previous reports of human and canine RBC swelling after manual or automated RBC washing. 46 , 51 The cellular swelling during washing is widely considered to be secondary to build up of osmotically active substances (lactate, chloride, and 2,3‐DPG) intracellularly. 49 , 52 These osmotically active substances develop in RBCs during storage and, after washing, the extracellular osmolality decreases significantly depending on the washing media used, 53 , 54 resulting in an intracellular shift of fluid.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, a storage‐related dysfunction of Na‐K‐ATPase could increase intracellular sodium concentration lead to an osmotic pull of water into the RBC. 34 , 38 , 46 In humans, washing with solutions containing dextrose, mannitol, or albumin that exhibit osmotic or colloidal oncotic effects improves red cell morphology as evidenced by normalized MCV and increased MCHC, as well as decreased erythrocyte water content and improved cell health. 23 , 24 , 51 , 54 …”

Section: Discussionmentioning

confidence: 99%

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Effect of washing units of canine red blood cells on storage lesions

Coll

Ross

Williams

et al. 2021

Veterinary Internal Medicne

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Background In humans, washing stored blood products before transfusion reduces storage lesions and incidence of transfusion reactions, but the effectiveness of washing canine blood is unknown. Objectives The objective was to determine if manually washing units of stored blood would reduce storage lesions without adversely affecting erythrocytes. We hypothesized that washing stored units would reduce concentrations of storage lesions and cause minimal erythrocyte damage. Animals Eight healthy research dogs. Methods Repeated measure cohort study. Units of whole blood were stored for 28 days and washed 3 times with 0.9% NaCl. Blood samples were collected before and after storage, after each wash, and after being held at a simulated transfusion temperature. Variables measured included CBC variables, blood gas analysis, erythrocyte morphology, mean corpuscular fragility (MCF), and eicosanoid concentrations. A Friedman's test was used to evaluate changes in variables ( P < .05 was considered significant). Results After the first wash, compared to values after storage, there was a significant decrease in potassium (4.3 mmol/L [4.0‐4.7] to 1.2 mmol/L [1‐1.6]; P < .0001, median [range]), lactate (1.45 mmol/L [1.07‐1.79] to 0.69 mmol/L [0.39‐0.93]; P = .002), and partial pressure carbon dioxide (102 mm Hg [80.2‐119.2] to 33.7 mm Hg [24.5‐44.5]; P < .0001), and increase in MCV (69.3 fL [65.7‐72.3] to 74 fL [69.6‐79.5]; P = .0003), and MCF (0.444 fL [0.279‐0.527] to 0.491 fL [0.43‐0.616]; P = .0006). Conclusions and Clinical Importance A single wash of stored whole blood significantly reduces most extracellular storage lesions, and additional washing might cause hemolysis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of washing units of canine red blood cells on storage lesions

Coll

Ross

Williams

et al. 2021

Veterinary Internal Medicne

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“…Other reported complications of autotransfusion include haemolysis secondary to prolonged exposure to serosal membrane and mechanical injury during collection and re-infusion. 10,22,23 Haemolysis results in the release of free haemoglobin, which can lead to acute kidney injury. To minimise the risk of mechanical injury to the red blood cells, aspiration was performed gently using low-suction pressure to minimise cell damage during the retrieval.…”

Section: Discussionmentioning

confidence: 99%

Twelve autologous blood transfusions in eight cats with haemoperitoneum

Cole

Humm

2018

Journal of Feline Medicine and Surgery

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Objectives The objectives of this study were to describe the clinical use and outcome of autologous transfusions in cats with intracavitary haemorrhage. Methods A retrospective descriptive study was performed. Computerised medical records of a single referral centre were searched for cats receiving an autotransfusion. Medical records were evaluated for underlying disease process, autotransfusion technique, autotransfusion volume, time period over which the autotransfusion was given, packed cell volume (PCV) pre- and post-autotransfusion, percentage rise in PCV, use of other blood products and any complications of the procedure. Survival to discharge and survival at 2 months was documented. Results Between July 2012 and March 2018 a total of 12 autotransfusions were performed in eight cats. All patients were diagnosed with haemoperitoneum. Four of the eight cats were diagnosed with abdominal neoplasia, three had postoperative haemorrhage and one had a traumatic haemoperitoneum. Three cats received more than one autotransfusion. Blood was collected using a 23 G butterfly catheter and 20 ml syringe in 7/12 collections, a 23 G needle and 20 ml syringe in 2/12 collections and directly into syringes from the open abdomen at the time of surgery in 3/12 collections. A median volume of 50 ml (range 25-80 ml) was collected and administered, meaning a median volume of 16.5 ml/kg (range 9-26 ml/kg) was administered. The autologous transfusions were given over a median of 3 h (0.25-6 h). Five cats were given another blood product alongside the autotransfusion. Median percentage PCV increase was 5% (range 1-7%). Anticoagulant was used in 5/12 autotransfusions. No clinically relevant adverse effects were reported. Six of the eight cats survived to discharge. Two month survival was 60% (3/5). Conclusions and relevance Autologous transfusion appears to be a safe and effective technique for stabilising cats with haemoperitoneum. This technique allows rapid and cheap provision of blood and avoids the need for an allogenic blood donor.

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“…Twelve one year-old female Yucatan mini-pigs were included in the study with a median (min-max) weight of 46.9 (45.75-48.25) kg in the visceral model (n = 5) and 48 (41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53) kg in the cardiac model (n = 7). Each animal was individually identified by an ear tag (since a few days after birth) to guarantee traceability.…”

Section: Data On Animalsmentioning

confidence: 99%

A novel autotransfusion device saving erythrocytes and platelets used in a 72 h survival swine model of surgically induced controlled blood loss

et al. 2022

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Background The purpose of this study was to develop a swine model of surgically induced blood loss to evaluate the performances of a new autotransfusion system allowing red blood cells and platelets preservation while collecting, washing and concentrating hemorrhagic blood intraoperatively. Methods Two types of surgically induced blood loss were used in 12 minipigs to assess system performance and potential animal complications following autotransfusion: a cardiac model (cardiopulmonary bypass) and a visceral model (induced splenic bleeding). Animal clinical and hematological parameters were evaluated at different time-points from before bleeding to the end of a 72-hour post-transfusion period and followed by a post-mortem examination. System performances were evaluated by qualitative and quantitative parameters. Results All animals that received the autotransfusion survived. Minimal variations were seen on the red blood cell count, hemoglobin, hematocrit at the different sampling times. Coagulation tests failed to show any hypo or hypercoagulable state. Gross and histologic examination didn’t reveal any thrombotic lesions. Performance parameters exceeded set objectives in both models: heparin clearance (≥ 90%), final heparin concentration (≤ 0.5 IU/mL), free hemoglobin washout (≥ 90%) and hematocrit (between 45% and 65%). The device treatment rate of diluted blood was over 80 mL/min. Conclusions In the present study, both animal models succeeded in reproducing clinical conditions of perioperative cardiac and non-cardiac blood loss. Sufficient blood was collected to allow evaluation of autotransfusion effects on animals and to demonstrate the system performance by evaluating its capacity to collect, wash and concentrate red blood cells and platelets. Reinfusion of the treated blood, containing not only concentrated red blood cells but also platelets, did not lead to any postoperative adverse nor thrombogenic events. Clinical and comparative studies need to be conducted to confirm the clinical benefit of platelet reinfusion.

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Evaluation of canine red blood cell quality after processing with an automated cell salvage device

Cited by 10 publications

References 41 publications

Effect of washing units of canine red blood cells on storage lesions

Effect of washing units of canine red blood cells on storage lesions

Twelve autologous blood transfusions in eight cats with haemoperitoneum

A novel autotransfusion device saving erythrocytes and platelets used in a 72 h survival swine model of surgically induced controlled blood loss

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