Evaluation of cassava plants generated by somatic embryogenesis in different stages of development using molecular markers

Zambrano, Mayra Andreina Osorio; Gamez, Elizabeth; Molina, Sandy; Infante, Diógenes

doi:10.2225/vol15-issue4-fulltext-3

Cited by 5 publications

(3 citation statements)

References 17 publications

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“…Plant regeneration via somatic embryogenesis has become an integral component of genetic transformation system in cassava [14,15]. Regeneration studies have shown that the frequency and efficiency of somatic embryogenesis are highly genotype-dependent, and not all cassava cultivars are amenable to somatic embryogenesis, regeneration and transformation, therefore, there is a need to optimise the generation of embryogenic structures for each cassava cultivar [16,17,18,19,20,21,22].…”

Section: Introductionmentioning

confidence: 99%

Primary Somatic Embryos from Axillary Meristems and Immature Leaf Lobes of Selected African Cassava Varieties

Opabode

2013

BBJ

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The study evaluated high-value African cassava varieties for primary somatic embryogenesis using axillary meristems (AM) and immature leaf lobes (LL) on piclorambased medium. The study was conducted at the Central Biotech Lab, International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria between 2006 and 2009. Completely randomized design with four replicates was used for the study. Using LL explants, there were significant (P=.05) differences in percent responding leaf lobes, percent explant with pre-embryogenic structure, PSEF and PSEE among cassava varieties. The PSEF of the only three varieties that produced mature somatic embryo were 93.6, 88.5 and 85.7% for TME 12, Kibaha and Albert, respectively. Similarly, significant (P=.05) differences existed among the varieties in percent enlarged axillary meristem, percent explant with pre-embryogenic structure, PSEF and PSEE when AM Research ArticleBritish Biotechnology Journal, 3(3): 263-273, 2013 264 was the explant. The PSEFs of the only three varieties that produced mature somatic embryo were 83.6, 77.5 and 72.7% for TME 12, Kibaha and Albert, respectively. The PSEF and PSEE of LL explant were greater than those of AM by an average of 86.1% and 82.7%, respectively. The study concluded that both AM and LL were good explants for production of primary somatic embryo in cassava. Keywords

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Section: Introductionmentioning

confidence: 99%

Primary Somatic Embryos from Axillary Meristems and Immature Leaf Lobes of Selected African Cassava Varieties

Opabode

2013

BBJ

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“…1), donde se apreciaron cinco grupos, y los valores obtenidos de la distancia genética. Se observaron diferencias entre las muestras por los patrones de bandas generados, es decir, polimorfismos en la secuencia de ADN (Osorio et al, 2012), que sugieren la existencia de variabilidad genética entre las muestras ( Fig. 1 y 2).…”

Section: Resultados Y Discusiónunclassified

“…Permiten la detección de variabilidad gené-tica, pues ofrecen información sobre las relaciones interespecíficas e intraespecíficas (Osorio, 2012). Empleados por su calidad y cantidad de información, fuerza y reproductibilidad.…”

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Diversidad genética de <i>Moniliophthora roreri</i> mediante Polimorfismo de Longitud de Fragmentos Amplificados (AFLPs)

Suárez-Contreras

2017

Rev. Colomb. Cienc. Hortic.

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RESUMENEl cultivo de cacao, está afectado por el fitopatógeno Moniliophthora roreri, que causa gran pérdida en la producción en Norte de Santander. Este estudio muestra los resultados obtenidos utilizando AFLPs de M. roreri. Con el kit para sistema de análisis II, estandarizado y aplicado a 56 muestras de M. roreri, aisladas de nueve municipios. La combinación que presentó mayor número de polimorfismos fue E-ACC/ M-CAA, con 100 bandas y un número menor de alelos (6), con la combinación E-ACG/ M-CAA se obtuvieron 90 bandas y un número de alelos mayor (23), y con la combinación E-AAC/ M-CAA se presentaron 94 bandas y alelos (12). El análisis de datos se realizó según el dendrograma de similitud o distancias genéticas de Nei, obteniéndose cinco grupos con una alta variabilidad entre las muestras, del 6,5 al 58%. Al combinar la información morfológica y molecular se determinó el nivel de diversidad genética de Moniliophthora roreri.

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Post-Harvest Practices, Constraints and Opportunities Along Cassava Value Chain in Kenya

Abong

Shibairo

Wanjekeche

et al. 2016

Curr Res Nutr Food Sci

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Despite its great potential as a food, feed and for industrial application, its processing and marketing remains economically unexploited. A cross-sectional baseline survey was undertaken in Western (Migori and Busia) and Coastal (Kilifi and Kwale) regions of Kenya between March 2013 and February 2014 using structured questionnaires to assess the post-harvest practices, opportunities and constraints in cassava processing. Results indicate that cassava processing is predominantly (58%) a women affair, males accounting for 42% with modal processors age being 32 years. Flour was the most common processed cassava product in the Coast (33%) while dried chips was highly produced in Migori and Busia in equal proportion of 43%. Other important products included cassava crisps and composite flour. Coast region had a greater diversity of products which are none existent in other regions. Most of the processing across the regions are small scale with workers mostly being the owners and activities take place in open yards with majority of these being in Busia (85 %) followed by Migori (67 %) and Coast region (57 %). Constraints during cassava processing were ranked in the following order: irregular and inadequate supply coupled with low seasonal demand for cassava and cassava products; high perishability of cassava roots; lack of value addition and processing tools; poor group dynamics, cohesion and management structure; lack of capacity building in production and processing technologies. There is very limited value addition to cassava in the study regions and hence the need to develop innovative technologies as well as new domestic and industrial products. Consumer sensitization and awareness on utilization of cassava and cassava products may be key to its promotion.

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Evaluation of cassava plants generated by somatic embryogenesis in different stages of development using molecular markers

Cited by 5 publications

References 17 publications

Primary Somatic Embryos from Axillary Meristems and Immature Leaf Lobes of Selected African Cassava Varieties

Primary Somatic Embryos from Axillary Meristems and Immature Leaf Lobes of Selected African Cassava Varieties

Diversidad genética de <i>Moniliophthora roreri</i> mediante Polimorfismo de Longitud de Fragmentos Amplificados (AFLPs)

Post-Harvest Practices, Constraints and Opportunities Along Cassava Value Chain in Kenya

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