Evaluation of Cellulase Activity in Enzymatic Hydrolysis Residues for Efficient Enzyme Reuse

Abstract

Enzymatic hydrolysis by cellulase has potential as an environment-friendly technology for producing bioethanol from lignocelluloses, but its production cost remains expensive. Therefore, several studies have examined methods in reducing the cost of enzymatic hydrolysis, and one of the most effective ways is to reuse enzymes. In this study, we developed a method for evaluating the cellulase activity in enzymatic hydrolysis residues for efficient enzyme reuse. Approximately 70% the of cellulase activity remained in the solid residue after 70% of the glucan was hydrolyzed, and 22% of cellulase activity still remained in the solid residue after 99% of glucan was hydrolyzed.Other experiments were performed in order to examine the application of our proposed method for practical reuse of enzymes. The results indicate that enzymes were recovered from the residues as we estimated by our method.Thus our method is efficient for evaluating cellulase activity in enzymatic hydrolysis residues.

A study on the enzymatic properties and reuse of cellulase immobilized with carbon nanotubes and sodium alginate

“…This may be because of the relatively weak binding forces via non covalent bonds. Some enzyme molecules cannot be effectively absorbed onto MWCNTs (Hasegawa et al 2016). The decreased activity may also be due to the hydrophilic characteristics of sodium alginate.…”

Production, Extraction, and Characterization of Green Biodegradable Bioplastics Polyhydroxybutyrates (PHB) for Biomedical Applications

“…Cellulase enzyme is by far one of the most effective enzymes to be used alone in the enzymatic hydrolysis (Kim et al, 2001;Hasegawa et al, 2016). A study conducted by Hasegawa et al, (2016) suggested that recycling cellulase enzymes after each enzymatic hydrolysis could solve the problem of their high cost as it was found that approximately 70% the of cellulase activity remained in the solid residue after 70% of the glucan was hydrolyzed, and 22% of cellulase activity remained in the solid residue after 99% of glucan was hydrolyzed. Multiple studies reached the fact that glucose release is controlled by removing of xylose and hemicellulose coating on cellulose.…”