Evaluation of chemopreventive effects of Thymoquinone on cell surface glycoconjugates and cytokeratin expression during DMBA induced hamster buccal pouch carcinogenesis

Rajkamal, G.; Suresh, K.; Sugunadevi, G.; Vijayaanand, M.A.; Rajalingam, K.

doi:10.3858/bmbrep.2010.43.10.664

Cited by 5 publications

(6 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chemoprevention is a novel and promising approach to control, inhibit or suppress the tumor promotion by using natural entities. A large number of phytochemicals ingested in human diet represent a chemopreventive agent which possess antimutagenic, anticarcinogenic, antioxidant and anti-cell proliferating properties (10).…”

Section: Introductionmentioning

confidence: 99%

Effect of Genistein and Oxaliplatin on Cancer Stem Cells in Oral Squamous Cell Carcinoma: An Experimental Study

Hussein¹,

El‐Sheikh

Darwish

et al. 2018

Alexandria Dental Journal

View full text Add to dashboard Cite

INTRODUCTION:Squamous cell carcinoma is one of the malignant diseases that affect the oral cavity worldwide. One of the theories regarding oral carcinogenesis is that tumor growth is dependent on cancer stem cells. Markers specific for these cells as CD44 have been investigated in hope of developing a deeper understanding for their role in carcinogenesis. Genistein, as chemopreventive agent, has been shown to suppress the growth of several tumors. Oxaliplatin is a chemotherapeutic compound that did show a range of antitumor activity. OBJECTIVES: This research was carried out to study the effect of genistein, oxaliplatin either alone or in combination during experimentally DMBA induced hamster buccal pouch carcinogenesis using CD44 antibody as a marker. MATERIALS AND METHODS: A total of 100 young Syrian hamsters distributed into groups as follows: 4 normal animals examined for the histology of the normal pouch mucosa and 96 animals divided into; group I, as a control group, in which pouches were painted with a heavy mineral oil only; group II were painted with DMBA mixed in a heavy mineral oil. These animals were randomly divided into 4 subgroups as following: group IIA only painted with DMBA; group IIB where genistein was orally administrated; group IIC were injected with oxaliplatin; and group IID in which both genistein and oxaliplatin were given. RESULTS: Both genistein and oxaliplatin provided a significant reduction in carcinogenesis process of DMBA induced oral squamous cell carcinoma. Moreover, they provided a significant decrease in the proliferation and activity of cancer stem cells as measured by the CD44 antibody. CONCLUSIONS: Genistein provides a chemoprevention role and the oxaliplatin produces a chemotherapeutic effect during the process of carcinogenesis. The combined action of both agents was better than the effect of each agent alone.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of Genistein and Oxaliplatin on Cancer Stem Cells in Oral Squamous Cell Carcinoma: An Experimental Study

Hussein¹,

El‐Sheikh

Darwish

et al. 2018

Alexandria Dental Journal

View full text Add to dashboard Cite

show abstract

“…These findings were in agreement with other studies (30,33) . Rajkamal et al (2010) (29) study indicates that TQ has potent chemopreventive efficacy in experimental oral carcinogenesis. Oral administration of TQ to DMBA painted hamsters significantly normalized and inhibited the abnormalities seen in cell surface glycoconjugates in the tumor tissues and circulation during carcinogenesis, and restored the expression of cytokeratin, which indicates their membrane stabilizing effect of TQ during neoplastic transformation.…”

Section: Discussionmentioning

confidence: 99%

“…Natural compounds are ubiquitously distributed throughout plants and marine organisms (23) . Chemoprevention is a promising approach to control, inhibit or suppress the tumor formation by using natural or synthetic entities, natural that possess antimutagenic, anticarcinogenic, antioxidant, anti-lipid peroxidative and anti-cell proliferating properties are considered as good chemopreventive agents in carcinogenesis including those of oral and experimental ones (6,(24)(25)(26)(27)(28)(29)(30) . A large number of phytochemicals ingested in human diet exhibit anticarcinogenic and antimutagenic effects (30).…”

Section: Introductionmentioning

confidence: 99%

Chemopreventive and therapeutic efficacy of thymoquinone on experimentally induced hamster buccal pouch carcinogenesis

Shamia

Abd-Alhafez

Soliman³

et al. 2022

ijhs

View full text Add to dashboard Cite

Aim: The present study aimed to investigate the chemopreventive and therapeutic effect of thymoquinone (TQ) on experimentally induced hamster buccal pouch carcinogenesis. Methodology : 40 Syrian male hamsters were classified into 4 equal groups (G(s)) of 10 each. GI: The animals remained untreated. The pouches of animals in GII, GIII, and GIV were painted 3 times a week for 14 weeks (ws). with 7,12-dimethylbenz (a) anthracene (DMBA). GII: No additional treatment was administered. Those in GIII: oral administration of TQ at a dose of 30 mg kg-1, one week before, as well as, during the application of DMBA on alternate days for 14 ws, whereas in GIV: The animals were injected intraperitoneally (IP) with TQ 50 mg/kg body weight thrice a week for 3ws. After the end of the experiment, the gross observations were made. Then, the fresh pouch specimens were surgically bisected into two pieces, one for Hematoxylin and Eosin (H&E) stain and immunohistochemistry (IHC) stain using Bcl-2. The other piece was used for apoptosis detection through a flow cytometry (FCM) test. Results: Gross observation showed variation in reduction of the tumor size in chemoprevention and treated group compared to the DMBA treated group.

show abstract

“…The development of tumors in Hamster buccal pouch exhibits various similarities such as shape, size, pathological features, pre-tumor lesions, property to spread and destruct,, biochemical and molecular markers expression and alterations in genomes as expressed in human OSCC [31]. In Hamster buccal pouch carcinogenesis, a sequential progression is observed through various degrees of dysplasia that is from hyperplasia to invasive carcinoma [32]. In DMBA induced hamsters 100% formation of tumor occurrence, tumor mass, tumor size and tumor progress were noted and subsequently counterchecked by performing histopathological analysis in which conditions like hyperplasia, dysplasia and squamous cell carcinoma are revealed [33,34].…”

Section: Discussionmentioning

confidence: 99%

Oral cancer preventive potential of Polydatin: A nanoencapsulation approach

Vijayalakshmi

Murali

Ghidan

et al. 2020

View full text Add to dashboard Cite

Over the period, several medicinal plants are recognized as attractive sources of anti-cancer molecules. About 35,000 plant organisms have been tested for possible anticancer activities by the National Cancer Center (NCI). Among them, reproducible anticancer behavior has been shown by around 3,000 plant organisms. Based on this information, we aimed to investigate the cancer preventive efficiency of Polydatin encapsulated PLGA nanoparticles (POL-PLGA-NPs) against 7,12 dimethylbenz (a) anthracene (DMBA) induced buccal pouch carcinogenesis by modulating the antioxidants process in male Syrian hamsters. Oral cancer was initiated on left side buccal pouches by applying DMBA (0.5%), (a known potent chemical carcinogen) on alternative days in a week (thrice a week) for a period of 14 weeks to provoke oral cancer. Sequences of oral squamous cell carcinoma are noted to be manifested exhibiting the formation of incidence, size, mass and development of tumor presented as preneoplastic and neoplastic lesions in hamsters oral buccal pouch tissues. At the same time, oral administration of POL-PLGA-NPs (30mg/kg.b.wt.) considerably increased glutathione peroxidase (GPx) and glutathione (GSH) levels in the buccal tissue thereby decreasing lipid peroxidation activity which resulted in neoplasms incidence in buccal tissue of DMBA painted animals. Thus, POL-PLGA-NPs activity seemed to be a potent cancer preventive agent.

show abstract

Evaluation of chemopreventive effects of Thymoquinone on cell surface glycoconjugates and cytokeratin expression during DMBA induced hamster buccal pouch carcinogenesis

Cited by 5 publications

References 39 publications

Effect of Genistein and Oxaliplatin on Cancer Stem Cells in Oral Squamous Cell Carcinoma: An Experimental Study

Effect of Genistein and Oxaliplatin on Cancer Stem Cells in Oral Squamous Cell Carcinoma: An Experimental Study

Chemopreventive and therapeutic efficacy of thymoquinone on experimentally induced hamster buccal pouch carcinogenesis

Oral cancer preventive potential of Polydatin: A nanoencapsulation approach

Contact Info

Product

Resources

About