Evaluation of chitosan-hydroxyapatite-collagen composite strength as scaffold material by immersion in simulated body fluid

Sari, Nurmala; Indrani, Decky Joesiana; Johan, Christian; Corputty, J.E.M.

doi:10.1088/1742-6596/884/1/012116

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…In general, it is seen that the samples produced in our study exhibit low mechanical properties. These values can be attributed to the probability of scaffold degradation, as reported by Sari et al 48 Chitosan and gelatin are soluble polymers in the SBF solution. After 7 days of immersion, the mechanical properties were affected more by polymer degradation than the influence of surface coating minerals from SBF.…”

Section: Resultsmentioning

confidence: 55%

Biomimetic mineralization of chitosan/gelatin cryogels and in vivo biocompatibility assessments for bone tissue engineering

Öfkeli

Demir

Bölgen

2020

J of Applied Polymer Sci

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The objective of this study is to develop biomimetic chitosan: gelatin (CH:Gel) cryogels for bone tissue engineering, combining the biological recognition of natural polymers with the distinguished interconnected porosity of cryogels, and biomimicking properties of bone like hydroxyapatite. The control of the biomineralization process onto biomaterials should be evaluated before clinical application. Therefore, the effect of chitosan and gelatin ratios on the final properties of the cryogels were investigated. FTIR, XRD, and SEM analysis indicated that the SBF coating exhibited similar characteristics to hydroxyapatite. The cryogels showed good biocompatibility with L929 mouse fibroblasts. Clinical outcomes and gross pathological examination showed that neither necrosis nor foreign body reaction was noted at the end of implantation. The biomimetically mineralized scaffold was found to be non‐irritant and non‐toxic for bone tissue. The biological performance and favorable properties demonstrated that the SBF coated CH:Gel cryogel can be a promising biomimetic scaffold for bone tissue engineering applications.

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Section: Resultsmentioning

confidence: 55%

Biomimetic mineralization of chitosan/gelatin cryogels and in vivo biocompatibility assessments for bone tissue engineering

Öfkeli

Demir

Bölgen

2020

J of Applied Polymer Sci

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Enhanced osteogenic proliferation and differentiation of human adipose-derived stem cells on a porous n-HA/PGS-M composite scaffold

Wang

Sun

Zhang

et al. 2019

Sci Rep

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This study explored the applicability, cellular efficacy, and osteogenic activities of porous nano-hydroxyapatite/Poly (glycerol sebacate)-grafted maleic anhydride (n-HA/PGS-g-M) composite scaffolds. Nuclear magnetic resonance (NMR) analyses indicated that approximately 43% of the hydroxide radicals in PGS were displaced by maleic anhydride. Resonance bands at 1036 cm −1 occurred in scaffolds containing nHA powders, and peak areas increased when n-HA weight increased in PGS-M-n-HA-0.4, PGS-M-n-HA-0.5, and PGS-M-n-HA-0.6 scaffolds. The n-HA/PGS-g-M composite scaffolds exhibited porous microstructure with average pore size of 150–300 µm in scanning electron microscopy (SEM) analysis. Differential scanning calorimetry (DSC) identified the glass transition temperature (Tg) as −25–30 °C, indicative of quality resilience. The modulus of compressibility increased when n-HA content increased. Interestingly, viability of human adipose-derived stem cells (hADSCs) in vitro and expression of the osteogenic related genes RUNX2 , OCN , and COL1A1 was enhanced in the n-HA/PGS-g-M composite scaffolds compared to those factors observed in PGS-g-M scaffolds. Finally, simulated body fluid (SBF) tests indicated more apatite deposits on the surface of n-HA/PGS-g-M scaffolds compared to PGS-g-M scaffolds. Overall, porous n-HA/PGS-g-M composite scaffolds possessed acceptable biocompatibility and mechanical properties, and they stimulated hADSC cell proliferation and differentiation. Given these qualities, the composite scaffolds have potential applications in bone tissue engineering.

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Bone remodeling using a three-dimensional chitosan - hydroxyapatite scaffold seeded with hypoxic conditioned human amnion mesenchymal stem cells

Kamadjaja

2021

Dent. J.

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Background: Bone regeneration studies involving the use of chitosan–hydroxyapatite (Ch-HA) scaffold seeded with human amnion mesenchymal stem cells (hAMSCs) have largely incorporated tissue engineering experiments. However, at the time of writing, the results of such investigations remain unclear. Purpose: The aim of this study was to determine the osteogenic differentiation of the scaffold Ch-HA that is seeded with hAMSCs in the regeneration of calvaria bone defect. Methods: Ch-HA scaffold of 5 mm diameter and 2 mm height was created by lyophilisation and desalination method. hAMSCs were cultured in hypoxia environment (5% oxygen, 10% carbon dioxide, 15% nitrogen) and seeded on the scaffold. Twenty male Wistar rat subjects (8 – 10 weeks, 200 - 250 grams) were randomly divided into two groups: control and hydroxyapatite scaffold (HAS). Defects (similar size to scaffold size) were created in the calvaria bone of the all-group subjects, but a scaffold was subsequently implanted only in the treatment group members. Control group left without treatment. After observation lasting 1 and 8 weeks, the subjects were examined histologically and immunohistochemically. Statistical analysis was done using ANOVA test. Results: Angiogenesis; expression of vascular endothelial growth factor; bone morphogenetic protein; RunX-2; alkaline phosphatase; type-1 collagen; osteocalcin and the area of new trabecular bone were all significantly greater in the HAS group compared to the control group. Conclusion: The three-dimensional Ch-HA scaffold seeded with hypoxic hAMSCs induced bone remodeling in calvaria defect according to the expression of the osteogenic and angiogenic marker.

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Evaluation of chitosan-hydroxyapatite-collagen composite strength as scaffold material by immersion in simulated body fluid

Cited by 3 publications

References 11 publications

Biomimetic mineralization of chitosan/gelatin cryogels and in vivo biocompatibility assessments for bone tissue engineering

Biomimetic mineralization of chitosan/gelatin cryogels and in vivo biocompatibility assessments for bone tissue engineering

Enhanced osteogenic proliferation and differentiation of human adipose-derived stem cells on a porous n-HA/PGS-M composite scaffold

Bone remodeling using a three-dimensional chitosan - hydroxyapatite scaffold seeded with hypoxic conditioned human amnion mesenchymal stem cells

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