Evaluation of chromogenic media for the isolation of vancomycin-resistant enterococci from stool samples

Asir, Kerry; Wilkinson, Katy; Perry, John D.; Reed, Robert H.; Gould, F.K.

doi:10.1111/j.1472-765x.2008.02517.x

Cited by 12 publications

(11 citation statements)

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“…All studies reported similar rates of recovery of both VRENFM and VRENFS from chromogenic agars to those from BEAV (1,2,(4)(5)(6)(7)10). CVRE appeared to perform better than the other chromogenic agars compared to BEAV, but a direct comparison is needed to provide the most accurate comparison.…”

mentioning

confidence: 88%

“…Confirmation of VRE using this medium requires 48 to 72 h. Chromogenic agars to detect VRE demonstrate promise (1)(2)(3)(4)(5)(6)(7)10). BBL CHROMagar VanRE (CVRE; BD Diagnostics, Sparks, MD) is a selective and differential chromogenic agar under development for the detection of vancomycin-resistant E. faecium (VRENFM) and vancomycin-resistant Enterococcus faecalis (VRENFS).…”

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confidence: 99%

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Evaluation of BBL CHROMagar VanRE for Detection of Vancomycin-Resistant Enterococci in Rectal Swab Specimens

et al. 2010

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A study was performed on 517 surveillance rectal swabs to evaluate a selective and differential chromogenic medium, the BBL CHROMagar VanRE (CVRE), which enables recovery and identification of VanA-and VanB-containing Enterococcus faecium (ENFM) and Enterococcus faecalis (ENFS) isolates. Compared to BBL Enterococcosel agar, a bile-esculin-azide-vancomycin (BEAV) agar, the initial overall sensitivity, specificity, and positive and negative predictive values of CVRE for the detection of vancomycin-resistant ENFM and ENFS were 99.1% and 94.8% and 84.2% and 99.7%, respectively. Among our patient population, more vancomycin-resistant enterococci (VRE) were recovered with CVRE than BEAV.Vancomycin-resistant enterococci (VRE) are major causes of nosocomial infections in health care facilities, and those patients infected with VRE have worse outcomes while hospitalized (8). Rapid, reliable identification of these antibioticresistant organisms is crucial for patient management and infection control measures (9, 12).Culture from rectal swabs or stool specimens onto bileesculin-azide agar with vancomycin (BEAV) is the VRE screening method used in many clinical laboratories. Confirmation of VRE using this medium requires 48 to 72 h. Chromogenic agars to detect VRE demonstrate promise (1-7, 10). BBL CHROMagar VanRE (CVRE; BD Diagnostics, Sparks, MD) is a selective and differential chromogenic agar under development for the detection of vancomycin-resistant E. faecium (VRENFM) and vancomycin-resistant Enterococcus faecalis (VRENFS). CVRE contains 8 g/ml of vancomycin and uses chromogenic substrates to phenotypically differentiate VRENFM as mauve colonies and VRENFS as green colonies. Other bacteria are inhibited or typically grow as a color other than mauve or green. Our study compared the clinical performance of CVRE with that of BEAV for primary isolation and detection of VRE from surveillance rectal swabs.Patient samples. This industry-sponsored clinical trial was approved by the Johns Hopkins University School of Medicine Institutional Review Board. At the Johns Hopkins Hospital, VRE surveillance cultures are obtained weekly from patients in all intensive care units (ICUs) and from other high-risk groups, such as oncology, transplant, and HIV patients. Multiple specimens per patient were permitted in the study if previous cultures were negative. Two positive specimens were admissible, provided the specimens were collected Ͼ5 days apart.Surveillance rectal swabs were first inoculated onto BEAV followed by inoculation onto CVRE. Both plates were aseptically streaked for isolation and incubated at 37°C for 24 to 48 h. BEAV. BEAV plates with 6 g/ml of vancomycin were incubated aerobically. No-growth cultures or those not consistent with VRE by 48 h had no further workup. Presumptive colonies for VRE (black colonies with a Gram stain of positive cocci) were isolated to 5% sheep blood agar (SBA) and incubated for an additional 18 to 24 h. L-Pyrrolidonyl-␤-naphthylamide enzyme (PYR)-positive colonies were identified with the BD ...

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confidence: 88%

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confidence: 99%

Evaluation of BBL CHROMagar VanRE for Detection of Vancomycin-Resistant Enterococci in Rectal Swab Specimens

et al. 2010

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“…Various chromogenic VRE agars appear promising for use in VRE stool screening (1,3,(4)(5)(6)11). Chromogenic VRE media can reduce turnaround time to results through early visual colony identification.…”

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confidence: 99%

Comparison of Performance of the Novel Chromogenic Spectra VRE Agar to That of Bile Esculin Azide and Campylobacter Agars for Detection of Vancomycin-Resistant Enterococci in Fecal Samples

2011

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A total of 142 stool specimens were evaluated for vancomycin-resistant enterococcus (VRE). Twenty-fourhour sensitivities and specificities, respectively, were 98% and 95% for Spectra VRE chromogenic agar (Remel, Lenexa, KS), 86% and 92% for bile esculin azide with vancomycin (BEAV; Remel), and 96.5% and 92% for Campylobacter agar (CAMPY; Remel). Spectra VRE and CAMPY are significantly more sensitive at 24 h than BEAV.

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“…Several formulations of chromogenic media have been developed to shorten this turnaround time and they are supposed to be used with or without enrichment step. Although many investigators reported that various chromogenic VRE agars appeared promising for use in VRE stool screening [3][4][5][6][7][8], only a few studies compared them with the method including the enrichment step [6].…”

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confidence: 99%

Comparison of Three Chromogenic Media for Recovery of Vancomycin-Resistant Enterococci from Rectal Swab Samples

Song

Park

et al. 2015

Ann Clin Microbiol

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Background: Three chromogenic media using direct inoculation were compared with enriched enterococcosel broth for vancomycin-resistant Enterococcus faecium and/or Enterococcus faecalis (VRE) surveillance. Methods: A total of 174 rectal swabs were included for VRE surveillance. The specimens were transferred in enterococcosel broth (EB). An aliquot of the broth was inoculated onto Brilliance VRE, chromID VRE, and VRESelect media and incubated for up to 48 h. We examined each media and EB after 24 h and 48 h of incubation. When appropriately colored colonies were observed, identification was confirmed using the VITEK-2 system and/or VITEK MS. Vancomycin susceptibility was confirmed by disk diffusion test. The presence of resistance genes was confirmed using Anyplex VanR Real-time Detection (Seegene, Korea

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Evaluation of chromogenic media for the isolation of vancomycin-resistant enterococci from stool samples

Cited by 12 publications

References 10 publications

Evaluation of BBL CHROMagar VanRE for Detection of Vancomycin-Resistant Enterococci in Rectal Swab Specimens

Evaluation of BBL CHROMagar VanRE for Detection of Vancomycin-Resistant Enterococci in Rectal Swab Specimens

Comparison of Performance of the Novel Chromogenic Spectra VRE Agar to That of Bile Esculin Azide and Campylobacter Agars for Detection of Vancomycin-Resistant Enterococci in Fecal Samples

Comparison of Three Chromogenic Media for Recovery of Vancomycin-Resistant Enterococci from Rectal Swab Samples

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