Evaluation of chromosome aberrations, sister chromatid exchange and micronuclei in patients with type-1 diabetes mellitus

Çinkılıç, Nilüfer; Kıyıcı, Sinem; Çelikler, Serap; Vatan, Özgür; Gül, Özen Öz; Tuncel, Ercan; Bilaloĝlu, Rahmi

doi:10.1016/j.mrgentox.2009.02.014

Cited by 37 publications

(21 citation statements)

References 22 publications

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“…The results obtained by us indicate a significant increase in the ratios of the SCEs in lymphocytes, which is in accordance with the previous reports (Cinkilic et al, 2009;Sheth et al, 2006). The SCEs are formed by toxic oxygen metabolites in cultured human leukocytes and other mammalian cells (Madrigal-Santillán et al, 2010).…”

Section: Discussionsupporting

confidence: 93%

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The ameliorative effect ofCetraria islandicaagainst diabetes-induced genetic and oxidative damage in human blood

Çolak

Geyikoğlu

Türkez

et al. 2013

Pharmaceutical Biology

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Context: The aqueous extracts of Cetraria islandica (L.) Ach. (Parmeliaceae) is traditionally used in many countries against a number of conditions, including inflammatory conditions. Objective: The present study aimed to assess, for the first time, the effectiveness of C. islandica in cultured primary blood cells of Type 1 diabetes subjects. Materials and methods: Diabetic and control blood samples were treated with or without aqueous lichen extract (5 and 10 mg mL À1) for 48 h. The activity of antioxidant enzymes in erythrocytes and also malondialdehyde levels in plasma were determined to evaluate the oxidative status. DNA damages were analyzed by SCE, MN and comet assays in cultured human lymphocytes. Additionally, proliferation index (PI) was evaluated in peripheral blood lymphocytes. Results: There were significant increases in observed total DNA damage (comet assay) (240.2%) and SCE (168.8%), but not in MN frequencies of cultures with diabetes as compared (p40.05) to controls. Whereas, the significant reductions of total DNA damage (69.2 and 65.3%) and SCE frequencies (17.7 and 12.3%) were determined when the 5 and 10 mg mL À1 lichen extract was added to the cell culture medium, respectively. However, lichen extract did not completely inhibit the induction of SCEs in lymphocytes of patients with diabetes. C. islandica extract was also useful on PI rates. Discussion: In conclusion, the antioxidant role of C. islandica in alleviating diabetes-induced genomic instability and for increasing cell viability was firstly indicated in the present study.

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Section: Discussionsupporting

confidence: 93%

“…In the present study, there were no differences in MN frequencies in the lymphocytes of T1D patients when compared with controls. Our current data confirm and expand previous findings by showing that patients with T1D have unchanged levels of MNs (Cinkilic et al, 2009).…”

Section: Discussionsupporting

confidence: 92%

The ameliorative effect ofCetraria islandicaagainst diabetes-induced genetic and oxidative damage in human blood

Çolak

Geyikoğlu

Türkez

et al. 2013

Pharmaceutical Biology

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“…In recent years, numerous researchers have investigated a number of genotoxicity parameters in relation to health and diseases [13]. In this population-based cross-sectional study, we observed an association between the increased frequency micronuclei in buccal epithelial cells and years exposure in workers.…”

Section: Discussionmentioning

confidence: 52%

Cytogenetic analysis of foundry molders using the micronucleus frequency in exfoliated buccal mucosa

Singaravelu¹

2018

A Rare Benign Lesion in the Lung: Clear Cell “Sugar” Tumor (CCST)

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“…It was reported that the genotoxicity of mercuric compounds including HgCl 2 indicated by the CA and MN tests in peripheral blood lymphocytes could be partly due either to the disturbance of the spindle mechanism, or to the elevated level of 8-OH-dG (a product of oxidatively damaged DNA) brought by the generation of ROS. In addition, some authors have suggested that ROS may be implicated in the production of high basal SCE frequencies in chromosome instability syndromes (Lee et al 1990;Therman and Susman 1993;Cinkilic et al 2009). Similarly, oxidative stress could lead to MN formations (Tsangaris et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Modulatory effects of Thymbra spicata L. different extracts against the mercury induced genotoxicity in human lymphocytes in vitro

2011

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Mercury, a xenobiotic metal, is a highly deleterious environmental pollutant. Moreover, in any form mercury is reported to be toxic. On the other hand, Thymbra spicata L., a member of the Lamiaceae family, has long been investigated popularly of biological roles; mainly antimicrobial and antioxidant activities. However, there are very scarce data on the cytogenetic effects of thyme species. The purpose of this study was to investigate the genetic safety of different extracts from T. spicata (water extract, methanol extract, and ethanol extract) and the effects of T. spicata on mercury (as HgCl 2 ) induced genotoxicity. Sister chromatid exchange (SCE) and micronucleus (MN) assays were performed to assess DNA damages in cultured human lymphocytes (n = 5). Our results clearly revealed that, the SCE and MN rates induced by HgCl 2 were alleviated by the presence of T. spicata. As conclusion, this study demonstrated for the first time that the T. spicata provided increased resistance of DNA against HgCl 2 induced genetic damage in human lymphocytes. Based on the results of this study, it may be concluded that the T. spicata is a nontoxic material that could be used as a suppressor of heavy metal-induced genotoxicity.

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Evaluation of chromosome aberrations, sister chromatid exchange and micronuclei in patients with type-1 diabetes mellitus

Cited by 37 publications

References 22 publications

The ameliorative effect ofCetraria islandicaagainst diabetes-induced genetic and oxidative damage in human blood

The ameliorative effect ofCetraria islandicaagainst diabetes-induced genetic and oxidative damage in human blood

Cytogenetic analysis of foundry molders using the micronucleus frequency in exfoliated buccal mucosa

Modulatory effects of Thymbra spicata L. different extracts against the mercury induced genotoxicity in human lymphocytes in vitro

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