Evaluation of circulatory and salivary levels of heat shock protein 60 in periodontal health and disease

Nethravathy, R Ramya; Alamelu, Swarna; Arun, KV; Kumar, Tamil Selvan

doi:10.4103/0970-9290.138317

Cited by 10 publications

(8 citation statements)

References 2 publications

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“…Surprisingly, there was no consistent association between salivary CRP and oral health parameters. In this regard, previous studies produced unequivocal results with both the absence (Redman et al 2016 ) (Torumtay et al 2016 ) and the presence (Nethravathy et al 2014 ; Shojaee et al 2013 ) of associations of between salivary CRP and the periodontal status. It is possible that an intense systemic inflammatory response in rheumatic disease overshadows that resulting from local lesions in the oral cavity.…”

Section: Discussionmentioning

confidence: 90%

Diagnostic value of salivary CRP and IL-6 in patients undergoing anti-TNF-alpha therapy for rheumatic disease

et al. 2018

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IntroductionSaliva has been increasingly used as a diagnostic medium for disease detection and monitoring. The aim of this observational, prospective, pilot study was to investigate whether salivary concentrations of CRP and IL-6 correlate with those in serum and with the clinical course of a rheumatic disease.Materials and methodsNineteen patients with rheumatic disease newly scheduled for anti-TNFα therapy were included. Patients received anti-TNFα treatment (adalimumab, certolizumab, golimumab or infliximab) as per standard protocols. CRP and IL-6 were measured with high-sensitivity immunoassays before and after 12 weeks of therapy, according to standard regimens. The data were analyzed with nonparametric statistics.ResultsConcentrations of CRP in saliva correlated significantly with those in serum (R = 0.62; p < 0.0001) and decreased markedly after successful response to treatment. In patients with a limited response to treatment salivary CRP levels increased. In contrast to CRP, the salivary concentrations of IL-6 did not change significantly over the course of therapy and they did not correlate with serum IL-6 concentrations. Salivary levels of neither CRP nor IL-6 corresponded to parameters of oral health and hygiene.ConclusionsSalivary CRP but not IL-6 could be of potential use for monitoring the rheumatic disease activity.

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Section: Discussionmentioning

confidence: 90%

Diagnostic value of salivary CRP and IL-6 in patients undergoing anti-TNF-alpha therapy for rheumatic disease

et al. 2018

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“…These endogenous components, including high‐mobility group box 1, ATP, S100 protein, heat shock protein and IL‐1 family proteins, served as alarmins and damage‐associated molecular patterns, are highly pro‐inflammatory in the extracellular milieu and are involved in innate immunity and inflammatory responses through the activation of pattern recognition receptors on host cells (Brydges et al, ; Chen & Nunez, ). According to previous studies, elevated level of extracellular high‐mobility group box 1, ATP, S100 protein and heat shock protein becomes the focus of attention in periodontitis (Binderman, Gadban, & Yaffe, ; Nethravathy, Alamelu, Arun, & Kumar, ; Sun et al, ; Tsybikov, Baranov, Kuznik, Malezhik, & Isakova, ; Xie, Deng, Gong, Ding, & Tang, ). Caspase‐3 rather than caspase‐1 is activated in the pyroptotic cell death aroused by butyrate, so IL‐1β and IL‐18 are not upregulated.…”

Section: Discussionmentioning

confidence: 99%

Butyrate rather than LPS subverts gingival epithelial homeostasis by downregulation of intercellular junctions and triggering pyroptosis

Liu

Wang

Meng

et al. 2019

J Clinic Periodontology

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Aim To investigate the effects of sodium butyrate (NaB) and lipopolysaccharide (LPS) on gingival epithelial barrier. Material and methods We cultured human primary gingival epithelial cells and investigated the effects of NaB and LPS on gingival epithelial barrier and involved mechanisms at in vitro and in vivo levels by immunostaining, confocal microscopy, field emission scanning electron microscopy (FE‐SEM), transmission electronic microscopy (TEM), transepithelial electrical resistance (TEER), FTIC‐dextran flux, flow cytometry, real‐time PCR and Western blot assays. Results Our results showed that NaB, rather than LPS, destroyed the epithelial barrier by breaking down cell–cell junctions and triggering gingival epithelial cell pyroptosis with characteristic morphological changes, including swollen cells, large bubbles, pore formation in the plasma membrane and subcellular organelles changes. The upregulated expression of pyroptosis‐related markers, caspase‐3 and gasdermin‐E (GSDME) contributed to this effect. Pyroptosis aroused by NaB is a pro‐inflammatory cell death. Pyroptotic cell death provoked inflammatory responses by upregulation of IL‐8 and MCP‐1, and releasing intracellular contents into the extracellular microenvironment after pyroptotic rupture of the plasma membrane. Conclusions Our new findings indicate that butyrate is a potent destructive factor of gingival epithelial barrier and pro‐inflammatory mediator, which shed a new light on our understanding of periodontitis initiation.

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“…GroEL proteins of the HSP60 family have been identified as major antigens in several periodontitis‐associated bacterial species , and a natural monoclonal IgM to oxidized LDL has been shown to recognize the HSP60 chaperon of A. actinomycetemcomitans . One study found salivary HSP60‐IgA to be higher in gingivitis than in periodontal health, but no difference was found in another study . Serum antibody titers to HSP90, HSP70, and P. gingivalis LPS were suggested to be useful in predicting the response to periodontal therapy .…”

Section: Molecular Mimicrymentioning

confidence: 99%

Mediators between oral dysbiosis and cardiovascular diseases

Pietiäinen

Liljestrand

Kopra

et al. 2018

European J Oral Sciences

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Clinical periodontitis is associated with an increased risk for cardiovascular diseases (CVDs) through systemic inflammation as the etiopathogenic link. Whether the oral microbiota, especially its quality, quantity, serology, and virulence factors, plays a role in atherogenesis is not clarified. Patients with periodontitis are exposed to bacteria and their products, which have access to the circulation directly through inflamed oral tissues and indirectly (via saliva) through the gastrointestinal tract, resulting in systemic inflammatory and immunologic responses. Periodontitis is associated with persistent endotoxemia, which has been identified as a notable cardiometabolic risk factor. The serology of bacterial biomarkers for oral dysbiosis is associated with an increased risk for subclinical atherosclerosis, prevalent and future coronary artery disease, and incident and recurrent stroke. In addition to species-specific antibodies, the immunologic response includes persistent, cross-reactive, proatherogenic antibodies against host-derived antigens. Periodontitis may affect lipoprotein metabolism at all levels, and all lipoprotein classes are affected. Periodontitis or its bacterial signatures may be involved not only in increased storage of proatherogenic lipids but also in attenuation of the anti-atherogenic processes, thereby putatively increasing the net risk of atherosclerosis. In this review we summarize possible molecular mediators between the dysbiotic oral microbiota and atherosclerotic processes.

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Evaluation of circulatory and salivary levels of heat shock protein 60 in periodontal health and disease

Abstract: Circulating HSP 60 levels may play a role in the systemic inflammatory state produced by periodontal disease. Salivary HSP 60 may not be used as a surrogate to determine systemic inflammation.

Cited by 10 publications

References 2 publications

Diagnostic value of salivary CRP and IL-6 in patients undergoing anti-TNF-alpha therapy for rheumatic disease

Diagnostic value of salivary CRP and IL-6 in patients undergoing anti-TNF-alpha therapy for rheumatic disease

Butyrate rather than LPS subverts gingival epithelial homeostasis by downregulation of intercellular junctions and triggering pyroptosis

Mediators between oral dysbiosis and cardiovascular diseases

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