Evaluation of Combined Ciprofloxacin and azithromycin free and nano formulations to control biofilm producing Pseudomonas aeruginosa isolated from burn wounds

Raouf, May; Essa, Sara; Achy, Samar El; Essawy, Marwa M.; Rafik, Salma T.; Baddour, Manal

doi:10.1016/j.ijmmb.2021.01.004

Cited by 10 publications

(8 citation statements)

References 24 publications

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“…The experimental result showed that the ciprofloxacin–azithromycin sinus stent (CASS) maintained a uniform coating and sustained delivery of ciprofloxacin and azithromycin, providing anti-biofilm activities against P aeruginosa ( Lim et al, 2020 ). Raouf et al (2021) indicated that combined free and ciprofloxacin–azithromycin nanoparticles on chitosan nanocarrier (Cipro-AZM-CS) showed promising results in vitro and in vivo overcoming high resistance of biofilm producing P. aeruginosa . The present study was conducted to evaluate the effect of azithromycin on P. aeruginosa biofilm.…”

Section: Discussionmentioning

confidence: 99%

Clinical Efficacy and In Vitro Drug Sensitivity Test Results of Azithromycin Combined With Other Antimicrobial Therapies in the Treatment of MDR P. aeruginosa Ventilator-Associated Pneumonia

et al. 2022

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Objective: The aim of the research was to study the effect of azithromycin (AZM) in the treatment of MDR P. aeruginosa VAP combined with other antimicrobial therapies.Methods: The clinical outcomes were retrospectively collected and analyzed to elucidate the efficacy of different combinations involving azithromycin in the treatment of MDR-PA VAP. The minimal inhibitory concentration (MIC) of five drugs was measured by the agar dilution method against 27 isolates of MDR-PA, alone or in combination.Results: The incidence of VAP has increased approximately to 10.4% (961/9245) in 5 years and 18.4% (177/961) caused by P. aeruginosa ranking fourth. A total of 151 cases of MDR P. aeruginosa were included in the clinical retrospective study. Clinical efficacy results are as follows: meropenem + azithromycin (MEM + AZM) was 69.2% (9/13), cefoperazone/sulbactam + azithromycin (SCF + AZM) was 60% (6/10), and the combination of three drugs containing AZM was 69.2% (9/13). The curative effect of meropenem + amikacin (MEM + AMK) was better than that of the meropenem + levofloxacin (MEM + LEV) group, p = 0.029 (p < 0.05). The curative effect of cefoperazone/sulbactam + amikacin (SCF + AMK) was better than that of the cefoperazone/sulbactam + levofloxacin (SCF + LEV) group, p = 0.025 (p < 0.05). There was no significant difference between combinations of two or three drugs containing AZM, p > 0.05 (p = 0.806). From the MIC results, the AMK single drug was already very sensitive to the selected strains. When MEM or SCF was combined with AZM, the sensitivity of them to strains can be significantly increased. When combined with MEM and AZM, the MIC50 and MIC90 of MEM decreased to 1 and 2 ug/mL from 8 to 32 ug/mL. When combined with SCF + AZM, the MIC50 of SCF decreased to 16 ug/mL, and the curve shifted obviously. However, for the combination of SCF + LEV + AZM, MIC50 and MIC90 could not achieve substantive changes. From the FIC index results, the main actions of MEM + AZM were additive effects, accounting for 72%; for the combination of SCF + AZM, the additive effect was 40%. The combination of AMK or LEV with AZM mainly showed unrelated effects, and the combination of three drugs could not improve the positive correlation between LEV and AZM.Conclusion: AZM may increase the effect of MEM or SCF against MDR P. aeruginosa VAP. Based on MEM or SCF combined with AMK or AZM, we can achieve a good effect in the treatment of MDR P. aeruginosa VAP.

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Section: Discussionmentioning

confidence: 99%

Clinical Efficacy and In Vitro Drug Sensitivity Test Results of Azithromycin Combined With Other Antimicrobial Therapies in the Treatment of MDR P. aeruginosa Ventilator-Associated Pneumonia

et al. 2022

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“…The mean particles' size as well as surface charge was measured by dynamic light scattering analysis and Zeta potential, respectively. Transmission electron microscope examination of the nanoparticles was conducted to assess the shape and distribution of the particles (22,23).…”

Section: Methods Synthesis and Characterization Of Curcumin Nanoparti...mentioning

confidence: 99%

Evaluation of Topical Application of Nanocurcumin Suspension in Treatment of Oral Candidal Infection (Experimental Study)

Abdelmonem

Moussa

Aboulela

et al. 2022

Alexandria Dental Journal

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INTRODUCTION:Oral candidiasis is the most common human fungal infection resulting from infection of the oral cavity by yeastlike fungus, candida. Its treatment by available topical or systemic antifungal drugs has several problems including toxicity and the development of drug resistance. Hence recent studies have given much attention to molecules from natural sources. Therefore, curcumin is considered one of the promising natural antifungal drugs. It has antifungal properties, but its poor solubility in aqueous solvents results in poor oral bioavailability. To improve the properties of curcumin, recent trails used nanotechnology. The size of nanoparticles can help them to gain entry into cells increasing its solubility and improving its bioavailability. OBJECTIVES: To evaluate the use of topical application of nanocurcumin in treatment of oral candidiasis and compare it to curcumin and nystatin in a murine model. MATERIALS AND METHODS: Thirty-nine female mice were randomly divided into three groups of 13 animals each after induction of oral candidiasis. Group 1 received nanocurcumin at 64 µg/ml. Group 2 received curcumin at 128 µg/ml. Group 3 received nystatin 100000 U/ml. All animals were received treatment topically twice daily for 10 days. The clinical evaluation and microbiological analysis were at baseline, day 5 and day 10. RESULTS: There was no statistical differences between the three studied groups in the number of cured animals at the end of the treatment period (p=0.358). CONCLUSION: Nanocurcumin has a good antifungal effect but further research is needed to get the maximum benefits of these nanoparticles.

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“… 121 In another study, Raouf et al noted that CIP/AZM combination, either in free form or as nanoparticles on a chitosan nanocarrier, showed promising results including improved survival, decreased bacteriological count, and better wound healing against CIP‐resistant biofilm‐producing P. aeruginosa strains. 122 …”

Section: Synergismmentioning

confidence: 99%

“…Lim et al also demonstrated that the attainable dose of AZM released from CASS showed significant anti‐inflammatory activity by successfully reducing LPS‐stimulated IL‐8 secreted from P. aeruginosa in human sinonasal epithelial cells without compromising their integrity 121 . In another study, Raouf et al noted that CIP/AZM combination, either in free form or as nanoparticles on a chitosan nanocarrier, showed promising results including improved survival, decreased bacteriological count, and better wound healing against CIP‐resistant biofilm‐producing P. aeruginosa strains 122 …”

Section: Synergismmentioning

confidence: 99%

Mechanism of action, resistance, synergism, and clinical implications of azithromycin

Heidary

Samangani

Kargari

et al. 2022

Clinical Laboratory Analysis

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Background Azithromycin (AZM), sold under the name Zithromax, is classified as a macrolide. It has many benefits due to its immunomodulatory, anti‐inflammatory, and antibacterial effects. This review aims to study different clinical and biochemisterial aspects and properties of this drug which has a priority based on literature published worldwide. Methods Several databases including Web of Science, Google Scholar, PubMed, and Scopus were searched to obtain the relevant studies. Results AZM mechanism of action including the inhibition of bacterial protein synthesis, inhibition of proinflammatory cytokine production, inhibition of neutrophil infestation, and macrophage polarization alteration, gives it the ability to act against a wide range of microorganisms. Resistant organisms are spreading and being developed because of the irrational use of the drug in the case of dose and duration. AZM shows synergistic effects with other drugs against a variety of organisms. This macrolide is considered a valuable antimicrobial agent because of its use as a treatment for a vast range of diseases such as asthma, bronchiolitis, COPD, cystic fibrosis, enteric infections, STIs, and periodontal infections. Conclusions Our study shows an increasing global prevalence of AZM resistance. Thus, synergistic combinations are recommended to treat different pathogens. Moreover, continuous monitoring of AZM resistance by registry centers and the development of more rapid diagnostic assays are urgently needed.

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Evaluation of Combined Ciprofloxacin and azithromycin free and nano formulations to control biofilm producing Pseudomonas aeruginosa isolated from burn wounds

Cited by 10 publications

References 24 publications

Clinical Efficacy and In Vitro Drug Sensitivity Test Results of Azithromycin Combined With Other Antimicrobial Therapies in the Treatment of MDR P. aeruginosa Ventilator-Associated Pneumonia

Clinical Efficacy and In Vitro Drug Sensitivity Test Results of Azithromycin Combined With Other Antimicrobial Therapies in the Treatment of MDR P. aeruginosa Ventilator-Associated Pneumonia

Evaluation of Topical Application of Nanocurcumin Suspension in Treatment of Oral Candidal Infection (Experimental Study)

Mechanism of action, resistance, synergism, and clinical implications of azithromycin

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