Pakistan is deficient in edible oil production. The sunflower being an important oilseed crop due to better oil composition, short duration as a cheapest source of energy and major component of our daily diet can be used for overcoming the gap. In this experiment combining ability was computed for achene yield and its related trait. 15 Genotypes of sunflower were crossed by using line × tester pattern. Out of 15 genotypes, A6, A7, A10, A11, A12, A17, A19, A20, A21 and A26 were taken as females (lines) and A8, A9, A16, A18 and A22 were taken as male (testers) in this experiment. 50 Crosses were made. The parental and F1 cross seeds was sown in randomized complete block design in 3 replications in next growing season. The data was recorded for number of leaves/plant, leaf area, plant height, head angle, fresh head diameter, dry head diameter, No of achenes/head, average yield/plant, 100 achene weight and harvest index. The recorded data was used to estimate the genetic variation among the genetic material. Line × tester analysis was used for estimating combining ability effects of sunflower genotypes. Analysis of variance, Line × Tester and Crosses vs parents showed highly significant differences among the sunflower accessions. Significant and positive GCA was found among lines A10, A21 for average yield and A17, A26 for 100 achene weight. Among testers significant and positive GCA values were found in A9 for average yield, A18 for 100 achene weight. Dominance variance represented that the ratio of GCA: SCA variance for all of the investigated traits is more than unity. All the lines had highest contribution to the total variance which indicated the presence of maternal effects. The findings suggest that crosses can produce heterotic effects which can be utilized for developing sunflower hybrids with high yield potential.