Evaluation of commercial herpes simplex virus IgG and IgM enzyme immunoassays

Liermann, Kristin; Schäfler, Anna; Henke, Andreas; Sauerbrei, Andreas

doi:10.1016/j.jviromet.2014.01.001

Cited by 29 publications

(23 citation statements)

References 21 publications

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“…Up to now, it remains very popular in routine analyses and in the develop ment and optimization of new methods as a reference method. It is used for the characterization of new immunoreagents, for the determination of stan dard/deviation concentration levels of particular com ponents in the detection of the absence/presence of pathology [13], the testing of the applicability of cur rently available immunoreagents and tests to the detection of new/specific forms of viruses [14], etc. In the classical version, ELISA is performed in micro plates with 96 or 384 wells.…”

Section: Immunochemical Methods Usedmentioning

confidence: 99%

Contemporary trends in the development of immunochemical methods for medical analysis

Goryacheva

2015

J Anal Chem

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The review is dedicated to contemporary trends in the development of immunochemical meth ods, the position of these methods in clinical analysis, and the applications of these methods. The tendencies toward miniaturization and the simultaneous determination of several analytes are considered. The prospects of applying nanosized markers, the requirements imposed on them, and limitations are discussed in detail. Predictions of the subsequent development of immunochemical methods in the area of clinical analysis are made.

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Section: Immunochemical Methods Usedmentioning

confidence: 99%

Contemporary trends in the development of immunochemical methods for medical analysis

Goryacheva

2015

J Anal Chem

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“…Alternatively to performing neutralization assays, which may require high amounts of purified antibodies, B-cells or cell-culture supernatants can be screened for antigen binding. Conventional ELISA assays are well established for routine diagnostics and usually based on purified viral antigens (Liermann et al, 2014). High throughput screening techniques based on antigen microarrays were developed to facilitate the screening process (De Masi et al, 2005;Tickle et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Detection of antibody-secreting cells specific for the cytomegalovirus and herpes simplex virus surface antigens

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Falk

Eis‐Hübinger

et al. 2018

Journal of Immunological Methods

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Infections with the herpes simplex virus (HSV) and the human cytomegalovirus (HCMV) can lead to life-threatening diseases, particularly in immunosuppressed patients. Furthermore, HSV infections at birth (herpes neonatorum) can result in a disseminated disease associated with a fatal multiorgan failure. Congenital HCMV infections can result in miscarriage, serious birth defects or developmental disabilities. Antibody-based interventions with hyperimmunoglobulins showed encouraging results in clinical studies, but clearly need to be improved. The isolation of highly neutralizing monoclonal antibodies is a promising strategy to establish potent therapy options against HSV and HCMV infections. Monoclonal antibodies are commonly isolated from hybridomas or EBV-immortalized B-cell clones. The screening procedure to identify virus-specific cells from a cell mixture is a challenging step, since most of the highly neutralizing antibodies target complex conformational epitopes on the virus surface. Conventional assays such as ELISA are based on purified viral proteins and inappropriate to display complex epitopes. To overcome this obstacle, we have established two full-virus based methods that allow screening for cells and antibodies targeting complex conformational epitopes on viral surface antigens. The methods are suitable to detect surface antigen-specific cells from a cell mixture and may facilitate the isolation of highly neutralizing antibodies against HSV and HCMV.

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“…However, the use of multiple assays to establish HSV type may increase the potential for aliquoting errors, in addition to the associated technologist and instrument time required for testing . HSV type‐common IgG tests provide reliable information on experienced HSV infection when there was no differentiation of HSV type‐specific immunity was required, such as HIV infection, organ transplantation, immunodeficiency and nologic inadequacy, the consequences of which were fatal . In addition, HSV type‐common IgG tests have been used widely when screening for HSV antibody in diagnostic laboratories .…”

Section: Discussionmentioning

confidence: 99%

Development of a time‐resolved fluorescence immunoassay for herpes simplex virus type 1 and type 2 IgG antibodies

et al. 2014

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Enzyme-linked immunosorbent assays (ELISA) specific for anti-HSV glycoprotein G (gG) are most commonly used in the clinical diagnosis of HSV infection. But most of them are qualitative and with narrow detection ranges. A novel time-resolved fluoroimmunoassay (TRFIA) methodology was developed for the quantitative determination of HSV IgG in human serum. The assay was based on an indirect immunoassay format, and performed in 96-well microtiter plates. HSV-1 and HSV-2 were used as the coating antigens. Eu(3+)-labeled goat anti-(human IgG) polyclonal antibodies were used as tracers. The fluorescence intensity of each well was measured and serum HSV IgG levels quantified against a calibration curve. The detection range of the novel TRFIA was between 5 and 500 AU/mL. Assay sensitivity was 0.568 AU/mL. The intra- and inter-assay coefficients of variation were 0.59-3.63% and 3.65-6.81%, respectively. Analytical recovery, dilution tests and serum panel tests were performed using TRFIA and the results proved satisfactory. There were no statistically significant differences in sensitivity and specificity between the TRFIA and commercial ELISAs. An effective, sensitive and accurate quantitative HSV type 1 and type 2 IgG TRFIA was successfully developed and provided diagnostic value in clinical use.

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Evaluation of commercial herpes simplex virus IgG and IgM enzyme immunoassays

Cited by 29 publications

References 21 publications

Contemporary trends in the development of immunochemical methods for medical analysis

Contemporary trends in the development of immunochemical methods for medical analysis

Detection of antibody-secreting cells specific for the cytomegalovirus and herpes simplex virus surface antigens

Development of a time‐resolved fluorescence immunoassay for herpes simplex virus type 1 and type 2 IgG antibodies

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