Evaluation of commercially available antisera for serotyping of<i>Pseudomonas aeruginosa</i>

Al-Dujaili, Ammar H.; Harris, D. M.

doi:10.1136/jcp.27.7.569

Cited by 9 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tipizacija izolata P. aeruginosa važna je zbog epidemioloških ispitivanja jer omogućava lakše praćenje prisustva pojednih serotipova, pomaže da se lociraju izvori infekcija i epidemija i prati rezistencija bacila 12 . Fenotipske metode tipizacije su: serotipizacija, biotipizacija, mikrobiološka ispitivanja osetljivosti na antibiotike, bakteriofag tipizacija, piocin tipizacija, multilokularna enzimska elektroforeza (MEE), masena spekrtofotometrija (MS), DNAb tipizacija i druge 13,14 . Genotipske metode su: direktna i reverzna hibridizacija, ribotipizacija, analiza restrikcionih endonukleaza (REA), PSR fingerprinting, analiza multilokularnih promenljivih gena, ispitivanje varijabilnih promenljiih parova (MLVA), tipizacija pojedinih sekvenci (SLST), genotipizacija profila plazmida (SNP) i druge 15 .…”

Section: Uvodunclassified

See 1 more Smart Citation

Serotyping and analysis of produced pigments kinds by Pseudomonas aeruginosa clinical isolates

Stanković-Nedeljković

Kocić²,

Tiodorović³

et al. 2011

VOJNOSANIT PREGL

View full text Add to dashboard Cite

show abstract

Section: Uvodunclassified

“…Izolati P. aeruginosa aglutinisani su sa triptikaza soja agara, slajd aglutinacionom tehnikom 14,16 . Za aglutinaciju korišćeni su polivalentni i monovalentni zečji antiserumi (Biorad).…”

Section: Aglutinacijaunclassified

Serotyping and analysis of produced pigments kinds by Pseudomonas aeruginosa clinical isolates

Stanković-Nedeljković

Kocić²,

Tiodorović³

et al. 2011

VOJNOSANIT PREGL

View full text Add to dashboard Cite

show abstract

“…K. pneumoniae (Podschun and Ullmann ), S. enterica (Wattiau et al . ), P. aeruginosa (Al‐Dujaili and Harris )). Antibodies specific for OPS have shown promise as vaccine antigens for several bacterial pathogens, whereby they have protected against challenge with the homologous pathogen in preclinical animal models (Svenson and Lindberg ; Watson et al .…”

Section: Introductionmentioning

confidence: 99%

Overexpression of O‐polysaccharide chain length regulators in Gram‐negative bacteria using the Wzx‐/Wzy‐dependent pathway enhances production of defined modal length O‐polysaccharide polymers for use as haptens in glycoconjugate vaccines

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The serotyping of the Pseudomonas aeruginosa strains isolated from various clinical materials is of great epidemiological importance, and distribution of the serotypes of P. aeruginosa has been reported throughout the world during the past 20 years (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). We have serotyped 668 P. aeruginosa strains isolated in Israel from diverse clinical specimens.…”

mentioning

confidence: 99%

Distribution of Pseudomonas aeruginosa Serotypes in Israel

Wellisch

Cohen

et al. 1980

Microbiology and Immunology

View full text Add to dashboard Cite

The serotyping of the Pseudomonas aeruginosa strains isolated from various clinical materials is of great epidemiological importance, and distribution of the serotypes of P. aeruginosa has been reported throughout the world during the past 20 years (1-15). We have serotyped 668 P. aeruginosa strains isolated in Israel from diverse clinical specimens. This is the first report dealing with the distribution of the serotypes of P. aeruginosa in Israel.The 668 P. aeruginosa strains were isolated from various clinical specimens such as urine, pus from wounds or ears, faeces, sputum or throat swab from respiratory tract or blood, in Rothschild University Hospital, Nahariya Government Hospital and Poriah Government Hospital, Israel, during the years 1972-1976. During this period there was no epidemic incidence of P. aeruginosa in any of these hospitals. The strains with the following characteristics were identified as P. aeruginosa: Gramnegative motile rods which oxidized glucose, but not maltose, reduced nitrate to nitrite, gave positive cytochrome-oxidase reaction ; they did not decarboxylyse lysine in lysine-iron agar, but dehydrolysed arginine in bacto arginine assay medium;4 gelatin was liquefied; indole was not formed; they did not produce H25 in TSI agar; gluconate was oxidized; aesculin was not hydrolysed.The antisera used were prepared as follows: the P. aeruginosa strains sent from Lanyi, representing his serotypes 01, 03a, 3b, 03a, 3d, 04a, 4b, 04a, 4d, 05a, 0b, 5c, 06, and 07a, 7b, were cultured on trypticase soya agar (Difco) containing 5% horse blood and incubated at 32 C for 18 hr. The culture was harvested, washed once with saline and then boiled for 2 hr. The boiled suspension was injected intravenously into rabbits, repeatedly with increasing dosages. Ten days after the last injection the rabbits were bled and the serum was stored at -20 C with 0.5% phenol.The slide agglutination test, using living cultures grown on nutrient agar at 233

show abstract

Evaluation of commercially available antisera for serotyping ofPseudomonas aeruginosa

Cited by 9 publications

References 8 publications

Serotyping and analysis of produced pigments kinds by Pseudomonas aeruginosa clinical isolates

Serotyping and analysis of produced pigments kinds by Pseudomonas aeruginosa clinical isolates

Overexpression of O‐polysaccharide chain length regulators in Gram‐negative bacteria using the Wzx‐/Wzy‐dependent pathway enhances production of defined modal length O‐polysaccharide polymers for use as haptens in glycoconjugate vaccines

Distribution of Pseudomonas aeruginosa Serotypes in Israel

Contact Info

Product

Resources

About