Serotyping and analysis of produced pigments kinds by Pseudomonas aeruginosa clinical isolates

Stanković-Nedeljković, Nataša; Kocić, Branislava; Tiodorović, Branislav; Branković, Srbobran; Mladenović-Antić, Snežana

doi:10.2298/vsp1111923s

Cited by 4 publications

(5 citation statements)

References 28 publications

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“…These findings are consistent with earlier reports showing dominance of the P3, P6, and P11 serotypes ( 28 , 29 , 30 ) and pigmentation where the synthesis of pyoverdine (79.75 %) was more pronounced than the synthesis of pyocyanin (44.14 %) ( 27 ). The pigments of the clinical Pseudomonas spp.…”

Section: Resultssupporting

confidence: 93%

“…Isolates from sputum samples (9.6 %), ear (4.3 %), tongue (2.1 %), and vaginal swabs (2.1 %) were rare. We already reported about their high prevalence in urine (36.79 %) and wounds (29 %) nearly ten years ago ( 27 ).…”

Section: Resultsmentioning

confidence: 99%

“…isolates participate in the development and maintenance of tissue lesions under inflammation in different ways. Therefore, the production of pigment is not only a phenotype characteristic but also an indicator of the level of pathogenicity of individual strains ( 27 ). However, a similar study in Serbia reported that only 2.45 % of 163 clinical P. aeruginosa isolates did not form a biofilm.…”

Section: Resultsmentioning

confidence: 99%

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Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia

Milojković

Nenadović

Stanković

et al. 2020

Archives of Industrial Hygiene and Toxicology

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Drug resistance of Pseudomonas aeruginosa is a leading problem in hospital infections. The aim of this study was to determine the best molecular genetic discrimination method for Pseudomonas spp. isolates among 94 outpatients and inpatients and see their grouping by phenotype characteristics (biofilm formation, frequency of serotypes, pigmentation, production of different class of beta-lactamases, and susceptibility to different antibiotic classes) and genotype. The most common serotypes were P1, P6, and P11, while co-productions of pyoverdine and pyocyanin were observed in 70 % of isolates. A total of 77.66 % isolates were mostly weak and moderate biofilm producers. Isolates were susceptible to colistin (100 %), aztreonam (97.87 %), imipenem (91.49 %), doripenem (90.43 %), and meropenem (84.04 %). MICs values confirmed susceptibility to ceftazidime and cefepime and singled out meripenem as the most effective inhibitor. Most isolates were resistant to aminoglycosides and fluoroquinolones. Only two isolates produced ESBL, eight were carbapenemase producers, and five isolates produced MBLs. Twenty-nine isolates were multidrug-resistant; 82.8 % of which produced both pigments, 58.3 % were non-typeable, while the P6 and P11 serotypes were equally distributed (16.7 %). Thirteen MDR isolates were strong enzyme producers. RAPD PCR analysis using primer 272 proved the best at discriminatory fingerprinting for Pseudomonas isolates, as it allocated 12 clusters. A correlation between DNA patterns and antibiotic resistance, production of pigments, serotypes distribution, and biofilm formation was not observed, and only confirmed higher genetic heterogeneity among P. aeruginosa isolates, which suggests that other molecular methods are needed to reveal potential relations between genotypic patterns and phenotypic characteristics.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia

Milojković

Nenadović

Stanković

et al. 2020

Archives of Industrial Hygiene and Toxicology

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“…In approximately 8% of cases, the standard method failed to detect serotype because of self-agglutinating or non-agglutinating strains and the samples were classified as “not typeable”. This proportion is less than those previously reported [11, 18, 27]. …”

Section: Discussioncontrasting

confidence: 69%

Pseudomonas aeruginosa serotypes in nosocomial pneumonia: prevalence and clinical outcomes

et al. 2014

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IntroductionPseudomonas aeruginosa frequently causes nosocomial pneumonia and is associated with poor outcome. The purpose of this study was to assess the prevalence and clinical outcome of nosocomial pneumonia caused by serotype-specific P. aeruginosa in critically ill patients under appropriate antimicrobial therapy management.MethodsA retrospective, non-interventional epidemiological multicenter cohort study involving 143 patients with confirmed nosocomial pneumonia caused by P. aeruginosa. Patients were analyzed for a period of 30 days from time of nosocomial pneumonia onset. Fourteen patients fulfilling the same criteria from a phase IIa studyconducted at the same time/centers were included in the prevalence calculations but not in the clinical outcome analysis.ResultsThe prevalence of serotypes was: O6 (29%), O11 (23%), O10 (10%), O2 (9%), and O1 (8%). Serotypes with a prevalence of less than 5% were found in 13% of patients, 8% were classified as not typeable. Across all serotypes, 19% mortality, 70% clinical resolution, 11% clinical continuation, and 5% clinical recurrence were recorded. Age and higher APACHE II (Acute Physiology and Chronic Health Evaluation II) were predictive risk factors associated with probability of death and lower clinical resolution for P. aeruginosa nosocomial pneumonia. Mortality tends to be higher with O1 (40%) and lower with O2 (0%); clinical resolution tends to be better with O2 (82%) compared to other serotypes. Persisting pneumonia with O6 and O11 was, respectively, 8% and 21%; clinical resolution with O6 and O11 was, respectively, 75% and 57%.ConclusionsIn P. aeruginosa nosocomial pneumonia, the most prevalent serotypes were O6 and O11. Further studies including larger group sizes are needed to correlate clinical outcome with virulence factors of P. aeruginosa in patients with nosocomial pneumonia caused by various serotypes; and to compare O6 and O11, the two serotypes most frequently encountered in critically ill patients.

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“…When evaluating the activity of hydrogen peroxide, no significant differences were observed between the different serotypes. The literature shows that the prevalence of P. aeruginosa serotypes varies from one hospital to another and from one country to another, with O6 and O11 being the most prevalent serotypes overall [31,32,33,34].…”

Section: Discussionmentioning

confidence: 99%

Study on the In Vitro Activity of Five Disinfectants against Nosocomial Bacteria

Montagna

Triggiano

Barbuti

et al. 2019

IJERPH

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Nosocomial infections cause significant morbidity and mortality worldwide, and the pathogenic organisms responsible for such infections can develop resistance to antimicrobial agents. Understanding the activity of disinfectants against clinical and environmental bacterial isolates is therefore crucial. We analysed the in vitro activity of five antimicrobial products (phenolic compounds, didecyldimethylammonium chloride (DDAC), sodium hypochlorite, isopropanol + ammonium compounds (IACs), hydrogen peroxide) against 187 bacterial strains comprising clinical isolates, as well as 30 environmental isolates of Pseudomonas aeruginosa from hospital water samples. Disk diffusion assays were employed to assess antimicrobial activity. Hydrogen peroxide was significantly more active (p < 0.0001) than the other disinfectants against all P. aeruginosa, Klebsiella pneumoniae, Enterococcus faecalis and Staphylococcus aureus strains. It was also the only disinfectant with activity against both clinical and environmental strains of P. aeruginosa. DDAC and IAC-based disinfectants were ineffective against Gram-negative strains, but showed significant activity (particularly IACs, p < 0.0001) against the Gram-positive strains. Compared with IACs, DDAC was significantly more active on E. faecalis and less active on S. aureus (p < 0.0001). Sodium hypochlorite and phenol compounds, by contrast, were inactive against all bacterial strains. The development of disinfection procedures that are effective against all microorganisms is essential for limiting the spread of nosocomial infections.

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Serotyping and analysis of produced pigments kinds by Pseudomonas aeruginosa clinical isolates

Abstract: P. aeruginosa was isolated most of the from urine, sputum and other materials. The majority often serovars were O1, O6 and O11. The most of isolates produced fluorescin (58.54%), while 22.94% producted pyocianin and 21.21% both pigments.

Cited by 4 publications

References 28 publications

Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia

Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia

Pseudomonas aeruginosa serotypes in nosocomial pneumonia: prevalence and clinical outcomes

Study on the In Vitro Activity of Five Disinfectants against Nosocomial Bacteria

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