Evaluation of Common Microscopic Techniques for Detection of Blastocystis Hominis

Aldahhasi, Waad T.; Toulah, Fawzia H; Wakid, Majed H.

doi:10.21608/jesp.2020.88748

Cited by 7 publications

(6 citation statements)

References 25 publications

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“…While the results showed that the control group in which the age group (18-27 years) is the most numerous with a percentage of (27.1%), while the age group (58-67 years) is the least in number at a rate of (4.3%).The results of the presented study almost agreed with a study conducted in the city of Samarra, in which it was mentioned that the most frequent infection with Blastocystis was in the age group (6-10) years(Mahmood and Khudher, 2018). While there is a recent study conducted in Saudi Arabia in which it was mentioned that the highest percentage of infections with the human Blastocyst parasite was in the age group (50-31) years, and this does not correspond to the results of our current study(Aldahhasi et al, 2020). The results of the current study are almost similar to the results of other studies presented by (Abdalla et al, 2014) and (Sayal et al, 2020).…”

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confidence: 69%

Immunological evaluation of IL-10 concentration level in sera of digestive disorders patients infected with Blastocystis hominis infection in Al-Najaf city

Sayl¹,

Yahya²

2022

ijhs

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Objectives: To evaluating the concentration level of IL-10 in serum of patients suffering from digestive disorders Blastocystis parasitic infection and compared with control group by using ELISA. Methadology: Case-control study design was conducted on 70 patients (33 males and 37 females) at Al-Sadr medical city and Al-Hakim General Hospital and Al-Sajad Hospital. A control group of 70 participitant (29 males and 41 females) who were without any history of disease were included. Their ages ranged between (6 - ≥ 60) years, during the period from the beginning of November 2021 to the end of February 2022. Results: The results showed (48.6%) of culture positive for B.hominis. The results of immunoassay showed mean difference ± standard error difference of IL-10 was (5.878 ± 10.462 Pg/ml) with a 95% confidence interval was (14.809-26.565) in intestinal disorder patients with significant difference (P˂0.001). Conclusion: Interleukin-10 concentration levels have a significant effect on the results of Blastocystosis patients suffering from digestive disorders.

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confidence: 69%

Immunological evaluation of IL-10 concentration level in sera of digestive disorders patients infected with Blastocystis hominis infection in Al-Najaf city

Sayl¹,

Yahya²

2022

ijhs

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“…are intestinal protozoan parasites that have several forms in lifecycle including vacuolar, granular, amoeboid, and cyst forms. Human to human transmission is common, through the fecal-oral route, which explains significantly higher rates of infection among food and animal handlers 1 , 2 .…”

Section: Introductionmentioning

confidence: 99%

Identification and Genetic Characterization of Blastocystis Species in Patients from Makkah, Saudi Arabia

Wakid¹,

WT²,

MN³

et al. 2022

IDR

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Background: Blastocystis species (sp.) are gastrointestinal protozoan parasites with high prevalence rates worldwide. Blastocystis sp. show extensive genetic diversity with 17 different subtypes (STs) described to date. A few studies have investigated the prevalence and STs of Blastocystis sp. in Makkah, Saudi Arabia. Therefore, we aimed in this study to identify and characterize subtypes of Blastocystis sp. in the City of Makkah, Saudi Arabia. Methods: Stool samples were collected from 140 patients who presented to King Abdulaziz Hospital, Hera General Hospital and Modern Medical Center in Saudi Arabia. Different microscopic examination methods of patients' stools and molecular analyses (using primers targeting SSU rRNA gene) were performed to identify and characterize STs of Blastocystis sp. Results: Our microscopic examination of stool samples showed that 96/140 patients (68.6%) had Blastocystis sp. infection. Clinical examination of infected patients revealed that 81 patients were symptomatic, whereas 15 were asymptomatic. Next, we isolated DNA from Blastocystis sp.-positive stool samples followed by PCR amplification of small-subunit ribosomal RNA (SSU rRNA) gene and sequence analysis. Our sequence analysis showed that subtype 3 (ST3) was the most prevalent (53.13%) followed by subtype 1 (ST1) (45.83%), whereas subtype 2 (ST2) was the least prevalent (1.04%). Moreover, our results showed that all three STs resulted in more symptomatic than asymptomatic cases. Finally, we identified novel haplotypes which comprised of 8 ST3, 6 ST1, and one ST2 haplotypes. Conclusion: Our identification of several haplotypes in patients' stools confirms the genetic diversity of Blastocystis sp. and may explain the reported low host specificity and differential pathogenicity of Blastocystis sp. We believe that additional molecular epidemiological and genomic studies are needed to understand the prevalence and pathogenicity of different subtypes in humans and animal hosts.

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“…Since many clinical laboratories do not report or identify this parasite, its prevalence could be higher than revealed in several studies. The pathogenicity of this parasite is still highly debated ( Yason et al, 2019 ; Aldahhasi, Toulah & Wakid, 2020 ). In the future, we intend to use molecular techniques to obtain more information about the genetic diversity of the isolated Blastocystis spp.…”

Section: Discussionmentioning

confidence: 99%

“…Blastocystis hominis is one of the most frequent intestinal protozoans in humans with the greatest prevalence in developing countries. However, there is still much debate about its pathogenicity ( Aldahhasi, Toulah & Wakid, 2020 ; Roberts et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Prevalence, type of infections and comparative analysis of detection techniques of intestinal parasites in the province of Belgarn, Saudi Arabia

Alqarni

Wakid

Gattan

2022

PeerJ

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Background The study was conducted to observe the prevalence and type of infection caused by intestinal parasites and to compare the techniques that are available for the detection. Method A total of 112 stool samples were obtained from study participants, and the laboratory examinations were performed at Special Infectious Agents Unit, King Abdulaziz University, Jeddah. One hundred and twelve participants were included in this study. Result The color of positive specimens was mainly brown (86.4%). Stool consistency in infected cases was soft in (64%) samples. There was no statistically significant difference with the physical characteristics of the stool (P > 0.05). In total, 59 of the 112 participants were infected with intestinal parasites, representing 52.7%. Different intestinal protozoa parasites were identified in which Blastocystis hominis (86.4%) was highest. None of the intestinal helminths were detected. Out of the 59 infected cases, single infections were found in (62.7%) samples. The intestinal protozoan parasites in single infections were B. hominis (78.4%), Giardia lamblia (8.1%), and (2.7%) from each Entamoeba histolytica, Cryptosporidium parvum, Entamoeba coli, Endolimax nana, and Chilomastix mesnili. Microscopy, RDTs, and real-time PCR were used for detection and identification of G. lamblia, E. histolytica, and C. parvum. Conclusion The study reported a high rate of intestinal parasitic infections, mainly with B. hominis. There were no statistical differences in parasite detection between the three techniques in detection of the thirteen cases infected with the pathogenic protozoa.

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Evaluation of Common Microscopic Techniques for Detection of Blastocystis Hominis

Cited by 7 publications

References 25 publications

Immunological evaluation of IL-10 concentration level in sera of digestive disorders patients infected with Blastocystis hominis infection in Al-Najaf city

Immunological evaluation of IL-10 concentration level in sera of digestive disorders patients infected with Blastocystis hominis infection in Al-Najaf city

Identification and Genetic Characterization of Blastocystis Species in Patients from Makkah, Saudi Arabia

Prevalence, type of infections and comparative analysis of detection techniques of intestinal parasites in the province of Belgarn, Saudi Arabia

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