Evaluation of crude hydatid cyst fluid antigens for the serological diagnosis of hydatidosis in cattle

Abstract

Echinococcosis is a zoonotic infection caused by adult or larval (metacestode) stages of cestodes belonging to the genus Echinococcus. The purpose of this study was to evaluate the antigenic ability of hydatid cyst fluid antigen for the diagnosis of hydatidosis in cattle using enzyme-linked immunosorbent assay (ELISA) and indirect haemagglutination test (IHA). The source of the antigens for the serological tests was fertile crude cyst fluids collected from naturally infected sheep at the Addis Ababa abattoir. A total of 502 sera were collected from 329 uninfected cattle and 173 hydatid-infected cattle which were confirmed by post-mortem examination. Most cysts were sterile and multiple organ infection predominated. Of 173 infected cattle, 166 (96.0%; confidence interval (CI) 91.8-98.4) were positive using ELISA while 7 (4.0%) were negative. Of 329 sera from uninfected cattle, 274 (83.3%; CI 78.8-87.2) were found to be negative and the remaining 55 (16.7%) were positive by ELISA. Of 173 infected cattle, 151 (87.3%; CI 81.4-91.9) were positive and 22 (12.7%) were negative by IHA. Of 329 negative sera tested using IHA, 266 (80.9%; CI 76.2-85.0) were negative and the remaining 63 (19.1%) were positive. The false positive and negative values of ELISA were 4.0 and 16.7%, respectively, and the corresponding values of IHA were 12.7 and 19.1%. The sensitivity and diagnostic efficiency of IHA were 87.2 and 83.6%, respectively. Crude hydatid cyst fluid antigen seems to have reasonable antigenic properties and hence could be employed for epidemiological surveillance of cattle hydatidosis.

Genotyping of Echinococcus granulose in Samples of Iraqi Patients

“…The success in PCR depends on the quantity and quality of the extracted DNA. There are some comparative studies of different DNA extraction methods forganisms, such as fungi, bacteria, viruses and protozoa (14)(15)(16)(17)(18).…”

Preliminary Evaluation of Recombinant EPC1 and TPx for Serological Diagnosis of Animal Cystic Echinococcosis

“…Cystic echinococcosis is a major zoonosis infecting humans and a wide range of animals, causing global health problems and economic losses worldwide (Sarkar et al, 2017). Diagnosis of CE by means of serology has limited support in clinical practice (Golassa et al, 2011;Jeyathilakan et al, 2014). Due to the poor specificity, complex composition and unstable antigen sources of natural proteins (Tamarozzi et al, 2014), establishing an accurate and sensitive immunologic diagnostic method based on recombinant protein has become crucial for CE detection.…”

Two Cases of Primary Splenic Hydatid Cyst in Greece

“…Hydatid cyst fluid lipoprotein antigen B (AgB) from E. granulosus is considered to be the most specific native or recombinant antigen for immunodiagnosis. The assay for human cysticercosis is based on a family of glycoproteins with molecular weight of 8 kDa with 98% sensitivity and 100% specificity [10]. …”