Genotyping of Echinococcus granulose in Samples of Iraqi Patients

Khalf, Maysara S.; –Faham, Mohammad A. Al; Al-Taie, Lazim H.; Alhussian, Hydar Aed

doi:10.9790/3008-09320610

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…Highest prevalence of CE was in the liver and the lungs, this is in agreement with the results of Ibrahim (2010), Hama (2013), Sargali and Mero (2013), AL-Bosely (2014), Mero et al (2014). This is because the liver act as the first filter for larval infection and the lungs as the second filter, and the oncosphere adopt the portal vein route and primarily negotiate hepatic and pulmonary filtering system sequentially before any other peripheral organ is involved (Kebede et al, 2009;Khalf et al, 2014;Mero et al, 2014;and Temam et al, 2016).…”

Section: Discussionsupporting

confidence: 89%

Prevalence of Echinococcus Granulosus in Different Intermediate Hosts in Duhok Province, Kurdistan Region, Iraq

Meerkhan

Mero

2018

SJUOZ

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This study was carried out in Duhok provinces, Kurdistan Region/ Iraq from August; 2014 to December 2015.The study focused on the prevalence of CE in slaughtered animals. The total prevalence was 5.25% (11753/223436), with a high rate in sheep (4.25%), and low rates in goats and cattle (0.64% and 0.37%, respectively). With respect to abattoirs involved in the study, the highest prevalence was detected in Akre abattoir (8.21%) and the lowest in Amedy abattoir (4.09%). Among the infected animals, sheep in Akre abattoir recorded the highest prevalence, while cattle in Duhok abattoir recorded the lowest prevalence (7.57 and 0.1%, respectively). Males of sheep, goats and cattle showed higher prevalence (70.51, 63.62, and 92.06%, respectively). Regarding organ involvements, malesand females of cattle showed high liver involvement (63.13 and 84.62%, respectively), while males and females of sheep (62.21 and 60.31%, respectively) and goats (60.59 and 54.51%, respectively) showed high lungs involvements.

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Section: Discussionsupporting

confidence: 89%

Prevalence of Echinococcus Granulosus in Different Intermediate Hosts in Duhok Province, Kurdistan Region, Iraq

Meerkhan

Mero

2018

SJUOZ

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“…The higher rate of liver and both (liver and lungs) involvement because liver act as the first filter for larval infection and the lungs act as the second filter, furthermore the liver possess the first great capillaries sites encountered by migrating the Echinococcus oncosphere (hexacanth embryo) which adopt the portal vein route and primarily negotiate hepatic and pulmonary filtering system sequentially before any other peripheral organ is involved (Kebede et al, 2009;Khalf et al, 2014;Mero et al, 2014 andQingling et al, 2014). The liver of sheep and goats were found to be more commonly infected with hydatid cysts than the lungs is in agreement with the previous findings of Saeed et al( 2000); Yildiz and Gurcan (2003); Haridy et al(2006); Kamhawi et al(2009);Saida and Nouraddin (2011);Jarjees and Al-Bakri(2012), and Khalf et al(2014). In contrast Sargali and Mero (2013) found 56.1% of HC in lungs and 36.25% and 7.66% in liver alone and both liver and lungs, and in goats, 67.31, 25.5 and 7.2% of HC were ISSN: 2410-7549 found in lungs, liver and both liver and lungs, respectively.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological Study of Cystic Echinococcosis in Sheep, Cattle and Goats in Erbil Province

Hassan

Mero

Casulli³

et al. 2016

SJUOZ

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Abstract:Cystic echinococcosis (CE) or hydatid cyst (HC) caused by the larval stage of the tapeworm Echinococcus granulosus continues to be a substantial cause of morbidity and mortality in many parts of the world. Since Kurdistan region is considered as endemic area, an epidemiological study was conducted to investigate the occurrence of CE isolated from sheep, cattle and goats from July 2013 to June 2014. The total rate of infection was 11.17(457/4092), with the highest rate (9.07%) in sheep and the lowest (0.54%) in goats. Regarding the sex of the infected animals, female showed slightly a higher rate than males (5.99% versus 5.18%) which statistically non-significant (P>0.05). Among the three intermediate host sheep showed the highest rate of infection (14.51%) regarding the sex females of sheep and cattle showed higher rate of infection versus males (27.36 and 20.35 versus 9.37 and 3.86). Furthermore, the highest incidence of CE in sheep, cattle and goats were found among the age group over three years (18.75, 5.41 and 1.69%, respectively), while the lowest rate was noted in the age groups less than one year (3.96 and 0.25%, o.33, respectively). On the other hand, sheep showed high rate of infection throughout the year with peaks during May to July which were 19.44%, 17.59 and 18.14%,respectively. This was followed by cattle and goats (11.67% and 5.26%) in April and December, respectively. Regarding the size, the higher numbers of small sized cysts were found in the liver (98/175), while the highest numbers of large sized cysts were found in both liver and lung which were 69/165. The liver and lungs appeared to be the site of predilection, since highest rate of infection was reported in these organs. Regarding the fertility of CE, the highest was in sheep (87.6%), followed by goats (77.27%) and then the cattle (40.63%).

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“…Results of the present works represented that, most of the isolates analyzed belonged to the G1 genotyping (sheep strain), and shown that this strain is actively transmitted throughout the Kurdistan region and surrounding countries [21][22][23], and the sheep play a significant role in human transmission cycle and the and the absence of G4, G6, and G7 genotype in this investigation is probably explained by the fact that in Kurdistan region, these genotype are specific to the species that were not slaughtered in slaughter house, like baffle, horse and pigs. The result disagreement with [24], who observed that in Iraq, the majority of CE patients (60) were infected with hydatid cysts of either G4 (43⁒), G5 (20⁒), and G6/G7 (7⁒) and [25] reported that, 38 isolates were sequenced by using polymerase chain reaction to amplify mitochondrial COX1 gene in the south-east of Iran and The genotype G1 was most prevalent in sheep (86.7%), cattle (80%), camels (44.4%), and goats (100%). Furthermore, [26] revealed that, of the 20 goat samples tested, 17 isolates (85%) were of the sheep strain's G1 genotype and the other three isolates (15%) were of the G6 genotype.…”

Section: -Discussionmentioning

confidence: 84%

Multiplex PCR Technique for Simultaneous Detection and genotyping of E. granulosus, E. multilocularis, and other Taeniidae in some intermediate hosts in Erbil Province

Hashm Hamad Abdullah,

Muhammad Jamal Muhammad,

Zuber Ismael Hassan

2024

Tikrit J. Pure Sci.

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One of the most significant helminthozoonosis in Iraq and around the world is cystic echinococcosis, which occur by the etiologic agent of Echinococcus granulosus and it’s distinguished by considerable intra-specific variability (genotypes G1–G10). DNA was extracted from 48 isolated cysts (25 sheep, 12 goats and 11 human) and used as templates to amplify using Trachsel method to differentiate Taeniide (Echinococcus spp. and/ or Taenia spp.) and mitochondrial cytochrome c oxidase subunit one (COX1) gene for genotyping. The PCR products were sequenced, and sequence analysis was used to further evaluate the data. Out of 48 separated of the host cysts, 36 isolates displayed the G1 genotype (100%), and in 12 samples, a nucleotide substitution at position 58 (T→C) results in polymorphism (99.9%). The G1 is considered that the most contagious and prevalent genotype of E. granulosus in the world. Our investigation revealed that, a single genotype that may be in charge of the disease's infectivity in sheep, goats, and human as well as its persistence in endemic areas. these epidemiological findings could be guided the successful hydatidosis control measures in Erbil Provence

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Genotyping of Echinococcus granulose in Samples of Iraqi Patients

Cited by 6 publications

References 25 publications

Prevalence of Echinococcus Granulosus in Different Intermediate Hosts in Duhok Province, Kurdistan Region, Iraq

Prevalence of Echinococcus Granulosus in Different Intermediate Hosts in Duhok Province, Kurdistan Region, Iraq

Epidemiological Study of Cystic Echinococcosis in Sheep, Cattle and Goats in Erbil Province

Multiplex PCR Technique for Simultaneous Detection and genotyping of E. granulosus, E. multilocularis, and other Taeniidae in some intermediate hosts in Erbil Province

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