Evaluation of Current Molecular Approaches for Genotyping of<i>Campylobacter jejuni</i>Strains

Ahmed, Monir Uddin; Dunn, Louise A.; Ivanova, Elena P.

doi:10.1089/fpd.2011.0988

Cited by 31 publications

(25 citation statements)

References 137 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, the results showed a distinct association between fla-SVR alleles and RFLP-PCR profiles for both C. jejuni and C. coli isolates. It has been reported that flaA gene based typing methods are not suitable for long-term epidemiological studies due to possible intra-and inter-genomic recombination between flagellin genes, but is a useful tool for Campylobacter genotyping, particularly initial screening of Campylobacter isolates [15,17,18,43] . Similarly, the results indicated that fla-SVR typing alone or in combination with other PCRbased methods can be used preliminary screening of campylobacter isolates in epidemiological studies.…”

Section: Aslantaşmentioning

confidence: 99%

Broilerlerden İzole Edilen Termofilik Campylabacter’lerin Genotipik, Antimikrobiyal Direnç ve Virulens Profilleri

Aslantaş¹

2017

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

The objective of this study was to investigate the prevalence, antimicrobial resistance and genetic determinants of resistance of Campylobacter jejuni and Campylobacter coli isolated from commercial broiler farms in Adana and Hatay provinces, Turkey. The assessment of the genetic diversity among the isolates was determined by flaA based RFLP-Polymerase Chain Reaction (PCR) with restriction enzyme DdeI and sequence analysis of short variable regions (SVRs) of flaA and flaB-SVR genes. Antimicrobial susceptibility of the isolates was performed by disk diffusion method and tetracycline (tetO), ampicillin (blaOXA-61), aminoglycoside (aph-3-1) and multidrug efflux pump (cmeB) resistance genes were investigated by PCR as well. The genes conferring resistance to ciprofloxacin was screened by PCR and following DNA sequencing. The presence of ten virulence (flaA, virB11, racR, cadF, ciaB, dnaJ and pldA) and toxin genes (cdtA, dtB, cdtC) among the isolates was also investigated using PCR. Out of 220 cloacal swabs, 218 (99.1%) Campylobacter spp. including C. jejuni (n=194; 89%) and C. coli (n=24; 11%) were isolated. While all the isolates were susceptible to chloramphenicol, gentamicin and erythromycin, resistance rates for C. jejuni and C. coli isolates to nalidixic acid, ciprofloxacin, ampicillin, tetracycline and amoxicillin-clavulanic acid were determined as 86.6-100%, 86.6-100%, 45.9-45.8%, 43.3-50% and 2.6-0.0%, respectively. The tetO, blaOXA-61, cmeB and aph-3-1 genes detected in C. jejuni and C. coli isolates were 45.3-54.2%, 36.1-75%, 1.5-83.3% and 0.5-0%, respectively.

show abstract

Section: Aslantaşmentioning

confidence: 99%

Broilerlerden İzole Edilen Termofilik Campylabacter’lerin Genotipik, Antimikrobiyal Direnç ve Virulens Profilleri

Aslantaş¹

2017

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

show abstract

“…A variety of subtyping methods are available for Campylobacter, with the most widely used being multilocus sequence typing (MLST) (4) and pulsed-field gel electrophoresis (PFGE) (5). Neither of these techniques is particularly rapid, with analysis times of several days (6,7). Data processing can also be complex (7,8).…”

mentioning

confidence: 99%

Same-Day Subtyping of Campylobacter jejuni and C. coli Isolates by Use of Multiplex Ligation-Dependent Probe Amplification–Binary Typing

et al. 2014

View full text Add to dashboard Cite

e Campylobacteriosis is the most commonly reported form of human bacterial gastroenteritis in the world. Sound identification of infectious sources requires subtyping, but the most widely used methods have turnaround times measured in days and require specialist equipment and skills. A multiplex ligation-dependent probe amplification-binary typing (MBiT) assay was developed for subtyping Campylobacter jejuni and Campylobacter coli. It was tested on 245 isolates, including recent isolates from Belgium and New Zealand, and compared to multilocus sequence typing (MLST). When used in an outbreak setting, MBiT identified the predominant genotype and possible additional cases days before pulsed-field gel electrophoresis (PFGE) results were available. MBiT was more discriminatory than MLST and, being a single assay with results produced within 6 h, was more rapid and cost-effective than both MLST and PFGE. In addition, MBiT requires only basic molecular biology equipment and skills.

show abstract

“…Tradicionalmente, el diagnóstico y confirmación de las especies de Campylobacter se ha realizado mediante cultivo bacteriológico y pruebas bioquímicas (Ewnetu y Mihret, 2010;Tadesse et al, 2011;Zaidi et al, 2012); sin embargo, en los últimos años, se han implementado técnicas moleculares como la reacción en cadena de la polimerasa (PCR) (Osail et al, 2012) y técnicas de genotipificación (Frederick y Huda, 2011, Ahmed et al, 2012Abley et al, 2012;Eberle y Kiess, 2012).…”

Section: Introductionunclassified

Diagnóstico molecular de Campylobacter en la cadena avícola destinada para consumo humano en Costa Rica.

et al. 2014

View full text Add to dashboard Cite

El objetivo de este estudio fue determinar la presencia y la frecuencia de Campylobacter jejuni y Campylobacter coli, en la cadena de producción avícola en Costa Rica. Se evaluaron granjas, planta de proceso y puntos de venta del Gran Área Metropolitana. Para la detección y aislamiento de Campylobacter sp. se analizaron 84 muestras provenientes de la cadena avícola (24 muestras de carne de pollo tomadas en punto de venta, 20 enjuagues de carcasa y 40 de ciegos tomados en planta de beneficio), obtenidas en noviembre de 2012, siguiendo el protocolo ISO 10272-1:2006 modificado por el Departamento de Agricultura de Estados Unidos (USDA, por sus siglas en inglés). De las 84 muestras analizadas, 36 (42,8%) resultaron positivas para C. jejuni, y una (1,2%) para C. coli. La cantidad de muestras positivas según la Reacción en Cadena de Polimerasa (PCR), por tipo de muestra fue: para contenido cecal, 16 (n=40), para carcasas, 8 (n=20) y para punto de venta 12 (n=24). Dieciséis de las veinte granjas muestreadas fueron positivas. Hubo una alta frecuencia de Campylobacter en todos los puntos de la cadena muestreados, lo que podría estar asociado a modificaciones de parámetros relacionados con inocuidad en cada eslabón. El enfriamiento rápido y el uso de cloro en el agua en la planta de proceso, disminuyó la frecuencia de muestras positivas de Campylobacter spp.

show abstract

Evaluation of Current Molecular Approaches for Genotyping ofCampylobacter jejuniStrains

Cited by 31 publications

References 137 publications

Broilerlerden İzole Edilen Termofilik Campylabacter’lerin Genotipik, Antimikrobiyal Direnç ve Virulens Profilleri

Broilerlerden İzole Edilen Termofilik Campylabacter’lerin Genotipik, Antimikrobiyal Direnç ve Virulens Profilleri

Same-Day Subtyping of Campylobacter jejuni and C. coli Isolates by Use of Multiplex Ligation-Dependent Probe Amplification–Binary Typing

Diagnóstico molecular de Campylobacter en la cadena avícola destinada para consumo humano en Costa Rica.

Contact Info

Product

Resources

About