Evaluation of Cytokine Production and Phagocytic Activity in Mice Infected with Campylobacter jejuni

Pancorbo, Pedro L.; Pablo, Manuel A. de; Ortega, Elena; Gallego, Aurelia M.; Álvarez, C.; Cienfuegos, Gerardo Álvarez de

doi:10.1007/s002849900433

Cited by 9 publications

(7 citation statements)

References 18 publications

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“…We demonstrated the usefulness of the gfp reporter system in an in vivo system by intraperitoneally injecting mice with C. jejuni that had been transformed with a gfp-containing vector. While mice do not represent a suitable model for Campylobacter-mediated enteritis, investigators have used intraperitoneal injection of mice to examine the interaction of C. jejuni with various components of the immune system (17,18,29). In conjunction with flow cytometry analysis, we demonstrate the application of the new vector containing gfp to show the cellular subset associated with C. jejuni in BALB/c mice.…”

mentioning

confidence: 99%

In Vivo Tracking ofCampylobacter jejuniby Using a Novel Recombinant Expressing Green Fluorescent Protein

Mixter

Klena

Flom

et al. 2003

Appl Environ Microbiol

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Campylobacter jejuni is a leading cause of food-borne disease in developed countries. The goal of this study was to develop a plasmid-based reporter system with green fluorescent protein (GFP) to facilitate the study of C. jejuni in a variety of niches. C. jejuni transformants harboring the pMEK91 GFP gene (gfp)-containing vector were readily detectable by both fluorescence microscopy and flow cytometry. Given the ease of detecting these organisms, additional experiments were performed in which BALB/c mice were injected intraperitoneally with C. jejuni harboring the gfp-containing vector. Four hours after injection of the mice, flow cytometry analyses determined that C. jejuni synthesizing GFP were predominantly associated with granulocytes. More specifically, the proportion of CD11b ؉ Gr-1 ؉ lavage neutrophils with green fluorescence ranged from 99.7 to 100%, while the proportion of CD11b ؉ Gr-1 ؊ lavage macrophages ranged from 77.0 to 80.0%. In contrast, few CD11b ؊ CD45R ؉ B lymphocytes from the lavage of the C. jejuni-injected mice were associated with greenfluorescent C. jejuni (proportions ranged from 0.75 to 0.77%). Cell-free C. jejuni was recovered from tissue homogenates after intraperitoneal injection. Macrorestriction profiling with pulsed-field gel electrophoresis identified a genotypic variant of the C. jejuni F38011 wild-type isolate. In vivo this variant displayed a phenotype identical to that of the wild-type isolate. In summary, we demonstrate that C. jejuni associates with marker-defined cellular subsets in vivo with a novel gfp reporter system and that C. jejuni genotypic variants can be isolated from both in vitro and in vivo systems.

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mentioning

confidence: 99%

In Vivo Tracking ofCampylobacter jejuniby Using a Novel Recombinant Expressing Green Fluorescent Protein

Mixter

Klena

Flom

et al. 2003

Appl Environ Microbiol

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“…C. jejuni is known to elicit proinflammatory cytokines, as determined in a number of in vitro studies (4,5,23,25,26,45), as well as in intraperitoneal models (2,38). The induction of proinflammatory cytokines was associated with the active invasion of bacteria in tissue culture models (4,27) and was related to the acute phase of Campylobacter gastroenteritis and the associated inflammatory pathology in the intestine in monkeys (44).…”

Section: Mouse Lung Model Of Infectionmentioning

confidence: 98%

“…Previous studies have indicated that an enterotoxigenic strain of C. jejuni could stimulate the production of IL-2, while it lacked the ability to induce the production of macrophage-derived cytokines (38,39). C. jejuni 81-176 could induce significantly higher levels of IL-2 in the lungs of infected mice (on day 1) (Fig.…”

Section: Mouse Lung Model Of Infectionmentioning

confidence: 99%

“…The roles of lipopolysaccharides and enterotoxins in the induction of proinflammatory cytokines have been demonstrated previously (4,9,38). These factors might also lead to neutrophil infiltration in the lungs (49) through the stimulation of IL-1␤ and TNF-␣ (21).…”

Section: Mouse Lung Model Of Infectionmentioning

confidence: 99%

“…Similarly, we observed a correlation between the increased levels of TNF-␣ and IFN-␥ and neutrophil accumulation in the lungs of the test mice during the early phase of infection. Phagocytic activity might have been occurring on day 1 without the significant production of IL-1␤, as demonstrated by the intraperitoneal inoculation of mice with C. jejuni (38).…”

Section: Mouse Lung Model Of Infectionmentioning

confidence: 99%

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Correlation of Proinflammatory and Anti-Inflammatory Cytokine Levels with Histopathological Changes in an Adult Mouse Lung Model ofCampylobacter jejuniInfection

Al-Banna

Albert

2008

Clin Vaccine Immunol

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Campylobacter jejuni is a major cause of diarrhea in humans. A mouse lung model of infection was previously established for C. jejuni. We used this model to study cytokine production in the lungs and correlated it with pathological changes. C. jejuni strain 81-176 or sterile phosphate-buffered saline was intranasally inoculated into adult BALB/c mice. The levels of proinflammatory cytokines (gamma interferon, tumor necrosis factor alpha, interleukin-1␤ [IL-1␤], IL-2) and anti-inflammatory cytokines (IL-4, IL-10), in addition to those of IL-6, were assessed on days 1, 3, and 5 postinfection by enzyme-linked immunosorbent assay, and the ratios of proinflammatory cytokines to anti-inflammatory cytokines were calculated. Since IL-6 is unique in that it is both a proinflammatory cytokine and a TH2 cytokine, it was considered to be both in the determination of these ratios. The significance of the cytokine levels and ratios were determined by the Mann-Whitney U test (P < 0.05). The induction of proinflammatory cytokines in the lungs of infected mice, as indicated by the cytokine levels and ratios, coincided with the accumulation of neutrophils and activated macrophages, in addition to the clearance of the bacterial load and bacteriumlike structures that we have previously shown in the same groups of mice. This was followed by increased levels of anti-inflammatory cytokines and the resolution of inflammation and pathology in the lungs. This study demonstrates the dynamics of cytokine production and their correlation with tissue inflammation and the resolution of infection. This model is useful for further studies of the pathogenesis of C. jejuni infection and vaccine evaluation.

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Potential Intervention of Campylobacter jejuni in the Modulation of Murine Immune Response

Pancorbo

Pablo

Ortega

et al. 2001

Current Microbiology

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Campylobacter jejuni has been reported to produce different toxins that may modulate the immune response in both animals and humans. The effect of C. jejuni enterotoxin on the immune response was investigated in two groups of Balb/c mice. One of them was inoculated intraperitoneally with 1010 colony forming units (CFU) of an enterotoxigenic strain (CCUG 7580), and the second one with a non-enterotoxigenic strain (CCUG 7440). The number of polymorphonuclear (PMN) cells from spleen increased in both enterotoxigenic and non-enterotoxigenic strains as a consequence of C. jejuni infection. Notwithstanding, lymphocyte proliferation stimulated by lipopolysaccharide (LPS) was increased by both enterotoxigenic and non-enterotoxigenic strains. Interleukin-2 (IL-2) production from splenic cells was increased significantly by infection with the enterotoxigenic strain. Both enterotoxigenic and non-enterotoxigenic strains reduced the splenic response to sheep erythrocytes; the response was significantly suppressed for immunoglobulin M (Ig M) and for immunoglobulin G (Ig G) synthesis. These results suggest that C. jejuni is able to modify some components of the immune response in mice, and also that the enterotoxigenic strain has more immunomodulating activity than the non-enterotoxigenic strain.

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Evaluation of Cytokine Production and Phagocytic Activity in Mice Infected with Campylobacter jejuni

Cited by 9 publications

References 18 publications

In Vivo Tracking ofCampylobacter jejuniby Using a Novel Recombinant Expressing Green Fluorescent Protein

In Vivo Tracking ofCampylobacter jejuniby Using a Novel Recombinant Expressing Green Fluorescent Protein

Correlation of Proinflammatory and Anti-Inflammatory Cytokine Levels with Histopathological Changes in an Adult Mouse Lung Model ofCampylobacter jejuniInfection

Potential Intervention of Campylobacter jejuni in the Modulation of Murine Immune Response

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