Evaluation of cytokines as adjuvants of infectious bovine keratoconjunctivitis vaccines

Girolamo, Fabio A. di; Sabatini, Diego Jorge; Fasan, Raúl A.; Echegoyen, Maia; Vela, Mauro; Pereira, Claudio A.; Maure, Pablo

doi:10.1016/j.vetimm.2011.12.022

Cited by 10 publications

(9 citation statements)

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“…For example, oral administration of chicken IFN-α inhibits avian influenza virus replication (Meng et al, 2011). In other economically relevant animals, such as cattle, the same cytokines were used in combination with inactivated bacteria improving the bovine conjuctival immune response to the pathogen Moraxella bovis, the causative agent of infectious bovine keratoconjunctivitis (di Girolamo et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

A Preventive Cytokine Treatment of the Viral Infectious Bursal Disease (IBD) of Chickens

et al. 2015

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Infectious bursal disease (IBD) is a viral disease of young chickens that produce severe lesions in the bursa of Fabricius and other organs inducing immunosuppression and mortality in birds. This study indicates that oral administration of IFN-α and IL-2 during 16 days produced a significant reduction in animals' morbidity and mortality to IBD virus (IBDV) infection accompanied with a decrease in symptoms and bursal tissue damage. The treatment also increased body weight, not only in birds challenged with IBDV, but also in uninfected controls. Infected birds treated with cytokines presented the same bursal index and organs' weight that controls; since untreated animals showed a significant decrease in these parameters. Finally, cytokine administration represents a new alternative to IBDV vaccination.

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Section: Resultsmentioning

confidence: 99%

A Preventive Cytokine Treatment of the Viral Infectious Bursal Disease (IBD) of Chickens

et al. 2015

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“…The TLR4 gene showed relatively high average expression in our samples, as did the IL6, and IL10 genes in most samples. IL6 was tested as a vaccine adjuvant for bovine infectious keratoconjunctivitis and showed high efficiency in the ocular treatment of infected cattle (Di Girolamo et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Innate immunity gene expression profiles in conjunctival membrane biopsies from Amazonian buffalo

et al. 2022

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Domestic buffalo production plays an economically important role in the Brazilian Amazon, but they are susceptible to many diseases favored by the tropical climate and annually flooded habitats, including ocular diseases. In this context, it is important to select genotypes that maximize innate ocular immunity in Amazonian herds. We aimed to characterise, for the first time, gene expression profiles of the innate immune system in the conjunctival membrane of buffalo. Ocular conjunctival tissue samples were collected from 60 clinically healthy slaughtered animals in the northern Brazilian state of Amapá. The samples were histologically processed for classification into three groups according to the quantitative degree of lymphoid tissue associated with the conjunctiva (discrete, G1; slight, G2; and moderate, G3 presence of lymphoid tissue). RT-PCR was used to quantify gene expression of inflammatory cytokine (IL6, IL10, TNFA, IFNG), Toll-like receptor 4 (TLR4), and Defensin beta 110 (DEFB110), relative to the endogenous GAPDH gene. G1 animals presented low expression for IL6, IL10, TNFA, and DEFB110, while G2 exhibited high expression for IL6, IL10, IFNG, and TLR4. All G3 animals showed high expression for all tested genes. These results suggest a greater resistance to pathogenic microorganisms of buffalos in the G3 group, and the proportion of lymphoid tissue associated with the conjunctiva may be related to the immune resistance of individuals.

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“…Other studies previously demonstrated the success of subcutaneously administered pilus-based vaccines at protecting calves against homologous but not heterologous challenges with M. bovis of different pilus serogroups (14), and it is possible that in the study reported by Zbrun et al (28), heterologous pilus strains were circulating in the study herd, which may have contributed to the development of IBK among vaccinates despite high tear antipilus IgA concentrations. In another recent study, calves were challenged 45 days following topical ocular vaccination with a killed M. bovis bacterin alone or with recombinant human interleukin 2 and alpha interferon (29). In that study, ocular antibody responses were not reported; however, calves that received the M. bovis bacterin plus cytokines had the lowest proportion of IBK and the lowest clinical IBK scores following challenge (29).…”

Section: Figmentioning

confidence: 97%

“…In another recent study, calves were challenged 45 days following topical ocular vaccination with a killed M. bovis bacterin alone or with recombinant human interleukin 2 and alpha interferon (29). In that study, ocular antibody responses were not reported; however, calves that received the M. bovis bacterin plus cytokines had the lowest proportion of IBK and the lowest clinical IBK scores following challenge (29).…”

Section: Figmentioning

confidence: 97%

“…Two of these studies evaluated an M. bovis bacterin administered by aerosol (26,27), one evaluated a native M. bovis pilus antigen administered by the intranasal (i.n.) route (28), and one evaluated an M. bovis bacterin administered by the intraocular route (29). To the author's knowledge, no published studies have evaluated ocular and systemic immune responses of cattle following i.n.…”

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Ocular Immune Responses in Steers following Intranasal Vaccination with Recombinant Moraxella bovis Cytotoxin Adjuvanted with Polyacrylic Acid

Angelos

Edman

Chigerwe

2013

Clin. Vaccine Immunol.

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Infectious bovine keratoconjunctivitis (IBK) caused by Moraxella bovis is the most common eye disease of cattle. The pathogenesis of M. bovis requires the expression of pili that enable the organism to attach to the ocular surface and an RTX (repeats in the structural toxin) toxin (cytotoxin or hemolysin), which is cytotoxic to corneal epithelial cells. In this pilot study, ocular mucosal immune responses of steers were measured following intranasal (i.n.) vaccination with a recombinant M. bovis cytotoxin adjuvanted with polyacrylic acid. Beef steers were vaccinated with either 500 g (n ‫؍‬ 3) or 200 g (n ‫؍‬ 3) of recombinant M. bovis cytotoxin plus adjuvant. Control group steers (n ‫؍‬ 2) were vaccinated with adjuvant alone, and all steers were given a booster on day 21. Antigen-specific tear IgA and tear IgG, tear cytotoxin-neutralizing antibody responses, and serum cytotoxin-neutralizing antibody responses were determined in samples collected prevaccination and on days 14, 28, 42, and 55. Changes in tear antigenspecific IgA levels from day 0 to days 28, 42, and 55 were significantly different between groups; however, in post hoc comparisons between individual group pairs at the tested time points, the differences were not significant. Our results suggest that i.n. vaccination of cattle with recombinant M. bovis cytotoxin adjuvanted with polyacrylic acid effects changes in ocular antigenspecific IgA concentrations. The use of intranasally administered recombinant M. bovis cytotoxin adjuvanted with polyacrylic acid could provide an alternative to parenteral vaccination of cattle for immunoprophylaxis against IBK. Infectious bovine keratoconjunctivitis (IBK) ("pinkeye") is the most common eye disease of cattle and causes corneal ulceration, corneal edema, blepharospasm, photophobia, and lacrimation in affected animals; young animals are most often affected. The disease occurs most commonly in cattle populations during summer periods in association with risk factors such as UV radiation, dust, plant awns, and flies. In severe cases, rupture of the cornea results in permanent blindness. Along with economic losses associated with treatment and prevention of IBK, there are individual animal costs associated with reduced animal well-being, comfort, and welfare. The etiologic agent of IBK has long been considered to be Moraxella bovis (1). In 2007, another species, Moraxella bovoculi, was reported that had been isolated from calves with IBK in northern California (2). Challenge inoculation of calves with M. bovoculi has not supported a direct causal role for M. bovoculi in corneal ulceration associated with IBK (3), and at this time, M. bovis remains the only organism for which Koch's postulates have been established with respect to IBK (1).The pathogenesis of M. bovis requires the expression of pilin for attachment to the corneal surface (4-6) and cytotoxin (hemolysin or cytolysin) that mediates damage to corneal epithelium, leading to ulceration (7-9). Pilus-based vaccines reduce the incidence and the severity of I...

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Evaluation of cytokines as adjuvants of infectious bovine keratoconjunctivitis vaccines

Cited by 10 publications

References 9 publications

A Preventive Cytokine Treatment of the Viral Infectious Bursal Disease (IBD) of Chickens

A Preventive Cytokine Treatment of the Viral Infectious Bursal Disease (IBD) of Chickens

Innate immunity gene expression profiles in conjunctival membrane biopsies from Amazonian buffalo

Ocular Immune Responses in Steers following Intranasal Vaccination with Recombinant Moraxella bovis Cytotoxin Adjuvanted with Polyacrylic Acid

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