Evaluation of Cytotoxicity and Genotoxicity ofAcacia aromaLeaf Extracts

Abstract

Acacia aroma, native plant from San Luis, Argentina, is commonly used as antiseptic and for healing of wounds. The present study was conducted to investigate the in vitro cytotoxicity and genotoxicity of hot aqueous extract (HAE) and ethanolic extract (EE) of A. aroma. The cytotoxic activity was assayed by neutral red uptake assay on Vero cell. Cell treatment with a range from 100 to 5000 μg/mL of HAE and EE showed that 500 μg/mL and 100 μg/mL were the maximum noncytotoxic concentrations, respectively. The CC50 was 658 μg/mL for EE and 1020 μg/mL for HAE. The genotoxicity was tested by the single-cell gel electrophoresis comet assay. The results obtained in the evaluation of DNA cellular damage exposed to varied concentrations of the HAE showed no significant genotoxic effect at range of 1–20 mg/mL. The EE at 20 mg/mL showed moderate genotoxic effect related to the increase of the DNA percentage contained in tail of the comet; DNA was classified in category 2. At concentrations below 5 mg/mL, the results of cytotoxicity and genotoxicity of aqueous and ethanolic extracts of Acacia aroma guarantee the safety at cell and genomic level. However further studies are needed for longer periods including animal models to confirm the findings.

Evaluation of Antimicrobial activity and Genotoxic Potential of Capparis spinosa (L.) Plant Extracts

“…Reviewing the scientific literature showed that this study is the first of its kind that studied the genotoxicity of C. spinosa extract on prokaryotes using molecular fingerprinting based on ERIC-PCR and RAPD-PCR techniques. Besides, many plants were previously examined to investigate their genotoxic potential using different techniques [21,22,28,[34][35][36][37][38][39]. In this study, RAPD-PCR and ERIC-PCR profiles showed many significant differences between the treated and untreated E. coli strain.…”

Evaluation of Genotoxic Effects of Alcoholic Extract of Kalanchoe Pinnata Using Single Cell Gel Electrophoresis

“…Using computerized image analysis system, the data were analyzed. By the ratio of tail to head length the extent of DNA damages was scored as follows: <5% -no damage, 5-20% low damage, 21-40% medium damage, 41-95% high damage, and >95% complete damage [15][16][17][18].…”

Heavy metal contamination and genotoxicity assessment of anthelmintic medicinal plants in Assam, India

“…A study by Nath et al (2017) revealed that Acorus calamus, used in folk medicine in India, caused genotoxic and hepatotoxic effects in its users. Several medicinal plants from Ethiopia, Argentina, Serbia, and parts of Africa have also been shown to produce genotoxic effects (Mattana et al 2014;Seukep et al 2014; Kahaliw et al 2018;Madić et al 2019). Inspite of their e cacy, medicinal plants can also be a source of toxic elements (Yuan et al 2011).…”