Evaluation of daidzein-loaded chitosan microcapsules for the colon cancer drug delivery: synthesis, characterization and release behaviour

Sanatkar, Robabbeh; Mohammad, Ghasem Rahimi Kalateh Shah; Karimi, Ehsan; Oskoueian, Ehsan; Hendra, Rudi

doi:10.1007/s00289-021-03853-0

Cited by 12 publications

(3 citation statements)

References 43 publications

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“…The CAM assay provides in vivo evidence of angiogenesis. The CAM treated with Chi-Trox NPs (10 μ mol) showed significant decrease in the blood vessel density, total blood vessel network, total blood vessel branch points, and total blood vessel nets ( p < 0.05) compared to the control and Trox (shown in Figure 8 and Table 3 ) The present study results were consistent with the previous reports [ 33 , 34 ].…”

Section: Resultssupporting

confidence: 92%

Antiangiogenic Potential of Troxerutin and Chitosan Loaded Troxerutin on Chorioallantoic Membrane Model

Subbaraj

Elangovan

Chandramouli

et al. 2023

BioMed Research International

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Angiogenesis is crucial to the development of cancer because it allows the transport of oxygen, nutrients, and growth factors as well as the spread of tumors to distant organs. Inhibitors of angiogenesis prevent the formation of blood vessels that allow tumor cells to shrink, rather than promote tumor growth. Chitosan acts as a carrier for many drugs, since the compound has various properties such as biodegradable, less toxicity, more stable, simple, easy to prepare, and biocompatible. The aim of the current study was to evaluate the efficacy of chitosan nanoparticles encapsulated with troxerutin (Chi-Trox NPs) against angiogenesis and cancer in ova chick chorioallantoic membrane (CAM) model. Chi-Trox NPs were synthesized using a nanoprecipitation method and were characterized by various analyses. 24 hours’ fertilized eggs (6 eggs/group) were treated with native Trox and Chi-Trox NPs for 5 days. The antiangiogenic activity was evaluated by morphometric, histopathological, immunohistochemical (CD104 and vimentin), and mRNA expression of MMP and FGF2 using RT-PCR. The anticancer activity was evaluated by histopathological, immunohistochmical (CD44), and mRNA expression of FGF2 and MMP. The synthesized chitosan NPs were successfully encapsulated with troxerutin, and the loading efficiency of chitosan NPs was found to be 86.4 ± 0.12 % and 13.2 ± 0.16 % respectively. Morphometric analysis of Chi-Trox NPs showed a considerable decrease in the number of blood vessels compared with control and native Trox. The histopathological observation of CAM confirmed that Chi-Trox NPs induce a significant reduction in inflammatory cells and the thickness of blood capillaries compared to control and native Trox. The immunohistochemical evaluation of CAM revealed Chi-Trox decreased CD104, vimentin and CD44 protein levels were compared with control and native Trox. Furthermore, the mRNA expression levels of FGF2 and MMP were significantly downregulated compared to their native forms. From the obtained results, Chi-Trox NPs possess significant inhibition of angiogenesis and can be used as therapeutic agents for cancer in the future.

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Section: Resultssupporting

confidence: 92%

Antiangiogenic Potential of Troxerutin and Chitosan Loaded Troxerutin on Chorioallantoic Membrane Model

Subbaraj

Elangovan

Chandramouli

et al. 2023

BioMed Research International

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“…Daidzein, a type of isoflavone found abundantly in legumes, particularly soy, 1,2 has been associated with various potential health benefits including antioxidant, 3,4 anti-inflammatory 5,6 and anti-cancer 7,8 properties. It is a small hydrophobic molecule soluble in polar solvents but with very low aqueous and lipid solubility.…”

Section: Introductionmentioning

confidence: 99%

“…25,26 The main disadvantage of these release triggers is their limited specificity where release of encapsulated compound can be unpredictable. Recently, Sanatkar et al 7 reported an approach to deliver daidzein to the colon using chitosan capsules susceptive to degradation by enzymes of colonic bacteria. They reported chitosan microcapsules with 64.3% entrapment efficiency and cumulative release of 38% in simulated gastric fluid (SGF, pH 4.5), 58% after subsequent treatment in simulated intestinal fluid (SIF, pH 6.8) and 79% in simulated colonic fluid ( pH 6.8-7).…”

Section: Introductionmentioning

confidence: 99%